The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein.
Open accessAbstract
The IRA1 gene is a negative regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. To identify other genes involved in this pathway, we screened yeast genomic DNA libraries for genes that can suppress the heat shock sensitivity of the ira1 mutation on a multicopy vector. We identified IRA2, encoding a protein of 3,079 amino acids, that is 45% identical to the IRA1 protein. The region homologous between the IRA1 protein and ras GTPase-activating protein is also conserved in IRA2. IRA2 maps 11 centimorgans distal to the arg1 locus on the left arm of chromosome XV and was found to be allelic to glc4. Disruption of the IRA2 gene resulted in (i) increased sensitivity to heat shock and nitrogen starvation, (ii) sporulation defects, and (iii) suppression of the lethality of the cdc25 mutant. Analysis of disruption mutants of IRA1 and IRA2 indicated that IRA1 and IRA2 proteins additively regulate the RAS-cyclic AMP pathway in a negative fashion. Expression of the IRA2 domain homologous with GAP is sufficient for complementation of the heat shock sensitivity of ira2, suggesting that IRA down regulates RAS activity by stimulating the GTPase activity of RAS proteins.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,533
Yanisch-Perron, C., Vieira, J., & Messing, J. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 15,041
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing
Henikoff, S. (1984) · Gene · doi:10.1016/0378-1119(84)90153-7 · Cited by 4,145
[12] One-step gene disruption in yeast
Rothstein, R. J. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)01015-0 · Cited by 3,204
Phosphorylation-Dephosphorylation of Enzymes
Krebs, E. G., & Beavo, J. A. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.004423 · Cited by 2,205
Carlson, M., & Botstein, D. (1982) · Cell · doi:10.1016/0092-8674(82)90384-1 · Cited by 1,495
A Cytoplasmic Protein Stimulates Normal N- ras p21 GTPase, But Does Not Affect Oncogenic Mutants
Trahey, M., & McCormick, F. (1987) · Science · doi:10.1126/science.2821624 · Cited by 1,211
Cited by
Weinberg, R. A. (1991) · Science · doi:10.1126/science.1659741 · Cited by 1,756
LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
Kabeya, Y., Mizushima, N., Yamamoto, A., Oshitani-Okamoto, S., Ohsumi, Y., & Yoshimori, T. (2004) · Journal of Cell Science · doi:10.1242/jcs.01131 · Cited by 1,394
The neurofibromatosis type 1 gene encodes a protein related to GAP
Xu, G., O'Connell, P., Viskochil, D., Cawthon, R., Robertson, M., Culver, M., Dunn, D., Stevens, J., Gesteland, R., White, R., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90024-9 · Cited by 1,095
The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins.
Ballester, R., Marchuk, D., Boguski, M., Saulino, A., Letcher, R., Wigler, M., & Collins, F. (1990) · Cell · doi:10.1016/0092-8674(90)90151-4 · Cited by 847
Stationary phase in the yeast Saccharomyces cerevisiae
Werner‐Washburne, M., Braun, E. L., Johnston, G. C., & Singer, R. A. (1993) · Microbiological Reviews · doi:10.1128/mmbr.57.2.383-401.1993 · Cited by 648
Metadata
- Authors
- Tanaka, K., Nakafuku, M., Tamanoi, F., Kaziro, Y., Matsumoto, K., & Toh-e, A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1990-08
- Volume
- 10
- Issue
- 8
- Pages
- 4303-4313
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1897167776
- Cited by
- 292
- References
- 29
- FWCI
- 9.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein. https://app.cochat.ai/papers/10.1128/mcb.10.8.4303
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- Tanaka, K., Nakafuku, M., Tamanoi, F., Kaziro, Y., Matsumoto, K., & Toh-e, A. (1990). IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.10.8.4303
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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