PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity.

Pongubala, J M; Nagulapalli, S; Klemsz, M J; McKercher, S R; Maki, R A; Atchison, M L

doi:10.1128/mcb.12.1.368

PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity.

PU.1 is a B-cell- and macrophage-specific transcription factor. By an electrophoretic mobility shift assay and dimethyl sulfate methylation interference assays, we show that PU.1 binds to DNA sequences within the immunoglobulin kappa 3' enhancer (kappa E3'). Binding of PU.1 to the kappa E3' enhan…