The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Characterization of a functional NF-kappa B site in the human interleukin 1 beta promoter: evidence for a positive autoregulatory loop.
Open accessAbstract
The -300 region of the interleukin 1 beta (IL-1 beta) promoter contains a functional NF-kappa B binding site composed of the decamer sequence 5'-GGGAAAATCC-3'. Probes representing the -300 region or the NF-kappa B site alone interacted with NF-kappa B proteins present in phorbol myristate acetate-, lipopolysaccharide-, or Sendai virus-induced myeloid cell extracts as well as recombinant NFKB1 (p50) and RelA (p65); furthermore, NF-kappa B protein-DNA complex formation was dissociated in vitro by the addition of recombinant I kappa B alpha. Mutation of the NF-kappa B site in the context of the IL-1 beta promoter reduced the responsiveness of the IL-1 beta promoter to various inducers, including phorbol ester, Sendai virus, poly(rI-rC), and IL-1 beta. A 4.4-kb IL-1 beta promoter fragment linked to a chloramphenicol acetyltransferase reporter gene was also preferentially inducible by coexpression of individual NF-kappa B subunits compared with a mutated IL-1 beta promoter fragment. When multiple copies of the IL-1 beta NF-kappa B site were linked to an enhancerless simian virus 40 promoter, this element was able to mediate phorbol ester- or lipopolysaccharide-inducible gene expression. In cotransfection experiments, RelA (p65) and c-Rel (p85) were identified as the main subunits responsible for the activation of the IL-1 beta NF-kappa B site; also, combinations of NFKB1 (p50) and RelA (p65) or c-Rel and RelA were strong transcriptional activators of reporter gene activity. The presence of a functional NF-kappa B binding site in the IL-1 beta promoter suggests that IL-1 positively autoregulates its own synthesis, since IL-1 is a strong inducer of NF-kappa B binding activity. Thus, the IL-1 beta gene may be considered as an important additional member of the family of cytokine genes regulated in part by the NF-kappa B/rel family of transcription factors.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Citations
Cites
Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
Gorman, C., Moffat, L. F., & Howard, B. H. (1982) · Molecular and Cellular Biology · doi:10.1128/mcb.2.9.1044 · Cited by 7,674
Smith, D. B., & Johnson, K. S. (1988) · Gene · doi:10.1016/0378-1119(88)90005-4 · Cited by 5,844
Interleukin-1 and interleukin-1 antagonism
Dinarello, C. A. (1991) · Blood · doi:10.1182/blood.v77.8.1627.1627 · Cited by 2,292
Osborn, L., Kunkel, S. L., & Nabel, G. J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2336 · Cited by 1,583
Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs
March, C. J., Mosley, B., Larsen, A., Cerretti, D. P., Braedt, G., Price, V. L., Gillis, S. A., Henney, C. S., Kronheim, S. R., Grabstein, K. H., et al. (1985) · Nature · doi:10.1038/315641a0 · Cited by 1,559
CYTOKINES: COORDINATORS OF IMMUNE AND INFLAMMATORY RESPONSES
Arai, K., Lee, F., Miyajima, A., Miyatake, S., Arai, N., & Yokota, T. (1990) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.59.070190.004031 · Cited by 1,318
Activation in vitro of NF-κB" by phosphorylation of its inhibitor IκB"
Ghosh, S., & Baltimore, D. (1990) · Nature · doi:10.1038/344678a0 · Cited by 1,150
NF-κB Controls Expression of Inhibitor IκBα: Evidence for an Inducible Autoregulatory Pathway
Sun, S., Ganchi, P. A., Ballard, D. W., & Greene, W. C. (1993) · Science · doi:10.1126/science.8096091 · Cited by 1,108
Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Auron, P. E., Webb, A. C., Rosenwasser, L. J., Mucci, S. F., Rich, A., Wolff, S. M., & Dinarello, C. A. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7907 · Cited by 1,042
The inducible transcription activator NF-κB: regulation by distinct protein subunits
Baeuerle, P. A. (1991) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/0304-419x(91)90007-8 · Cited by 1,025
Flagged first, then by citations
Cited by
Sorted by citations
Schroder, K., & Tschopp, J. (2010) · Cell · doi:10.1016/j.cell.2010.01.040 · Cited by 5,475
Succinate is an inflammatory signal that induces IL-1β through HIF-1α
Tannahill, G. M., Curtis, A. M., Adamik, J., Palsson-McDermott, E. M., McGettrick, A. F., Goel, G., Frezza, C., Bernard, N. J., Kelly, B., Foley, N. H., et al. (2013) · Nature · doi:10.1038/nature11986 · Cited by 4,176
Activators and target genes of Rel/NF-κB transcription factors
Pahl, H. L. (1999) · Oncogene · doi:10.1038/sj.onc.1203239 · Cited by 3,727
Inflammatory networks during cellular senescence: causes and consequences.
Freund, A., Orjalo, A. V., Desprez, P.-Y., & Campisi, J. (2010) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2010.03.003 · Cited by 1,354
Scheinman, R. I., Gualberto, A., Jewell, C. M., Cidlowski, J. A., & Baldwin, A. S. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.2.943 · Cited by 819
Metadata
- Authors
- Hiscott, J., Marois, J., Garoufalis, J., D'Addario, M., Roulston, A., Kwan, I., Pepin, N., Lacoste, J., Nguyen, H., & Bensi, G.
- Affiliations
- Lady Davis Institute for Medical Research (CA)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1993-10
- Volume
- 13
- Issue
- 10
- Pages
- 6231-6240
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC364682
- OpenAlex
- W1904705252
- Cited by
- 585
- References
- 68
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 6 · 2023: 19 · 2022: 18 · 2021: 26 · 2020: 24 · 2019: 16 · 2018: 26 · 2017: 20 · 2016: 28 · 2015: 32 · 2014: 22 · 2013: 21 · 2012: 17
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- IL-1β, NF-kappaB binding site, positive autoregulation, RelA/p65, c-Rel, IκBα, transcriptional activation, phorbol ester, lipopolysaccharide, Sendai virus, cytokine gene regulation, transcription factor-DNA complex
- MeSH terms
- Base Sequence, Binding Sites, Cell Line, DNA, Gene Expression Regulation, Humans, Interleukin-1, Molecular Sequence Data, Promoter Regions, Genetic, Proto-Oncogene Proteins, Transcription Factors, Tumor Cells, Cultured, Transcriptional Activation, NF-kappa B, Transcription Factor RelB
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
