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A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity.
Open accessAbstract
We identified a serine/threonine protein kinase that is associated with and phosphorylates phosphoinositide 3-kinase (PtdIns 3-kinase). The serine kinase phosphorylates both the 85- and 110-kDa subunits of PtdIns 3-kinase and purifies with it from rat liver and immunoprecipitates with antibodies raised to the 85-kDa subunit. Tryptic phosphopeptide maps indicate that p85 from polyomavirus middle T-transformed cells is phosphorylated in vivo at three sites phosphorylated in vitro by the associated serine kinase. The 85-kDa subunit of PtdIns 3-kinase is phosphorylated in vitro on serine at a stoichiometry of approximately 1 mol of phosphate per mol of p85. This phosphorylation results in a three- to sevenfold decrease in PtdIns 3-kinase activity. Dephosphorylation with protein phosphatase 2A reverses the inhibition. This suggests that the association of protein phosphatase 2A with middle T antigen may function to activate PtdIns 3-kinase.
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Cited by
Fruman, D. A., Meyers, R. E., & Cantley, L. C. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.481 · Cited by 1,355
Self-citationThe Role of Phosphoinositide 3-Kinase Lipid Products in Cell Function
Rameh, L. E., & Cantley, L. C. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.13.8347 · Cited by 987
Self-citationPhosphoinositide 3-kinase: the key switch mechanism in insulin signalling
SHEPHERD, P. R., WITHERS, D. J., & SIDDLE, K. (1998) · Biochemical Journal · doi:10.1042/bj3330471 · Cited by 953
Morris, J. Z., Tissenbaum, H. A., & Ruvkun, G. (1996) · Nature · doi:10.1038/382536a0 · Cited by 891
Hartley, K. O., Gell, D., Smith, G. C. M., Zhang, H., Divecha, N., Connelly, M. A., Admon, A., Lees-Miller, S. P., Anderson, C. W., & Jackson, S. P. (1995) · Cell · doi:10.1016/0092-8674(95)90482-4 · Cited by 744
Metadata
- Authors
- Carpenter, C. L., Auger, K. R., Duckworth, B. C., Hou, W. M., Schaffhausen, B., & Cantley, L. C.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1993-03
- Volume
- 13
- Issue
- 3
- Pages
- 1657-1665
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2130716490
- Cited by
- 206
- References
- 0
- FWCI
- 10.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Carpenter, C. L., Auger, K. R., Duckworth, B. C., Hou, W. M., Schaffhausen, B., & Cantley, L. C. (1993). A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.13.3.1657
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