The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Molecular Cloning of Apobec-1 Complementation Factor, a Novel RNA-Binding Protein Involved in the Editing of Apolipoprotein B mRNA
Open accessAbstract
The C-to-U editing of apolipoprotein B (apo-B) mRNA is catalyzed by a multiprotein complex that recognizes an 11-nucleotide mooring sequence downstream of the editing site. The catalytic subunit of the editing enzyme, apobec-1, has cytidine deaminase activity but requires additional unidentified proteins to edit apo-B mRNA. We purified a 65-kDa protein that functionally complements apobec-1 and obtained peptide sequence information which was used in molecular cloning experiments. The apobec-1 complementation factor (ACF) cDNA encodes a novel 64.3-kDa protein that contains three nonidentical RNA recognition motifs. ACF and apobec-1 comprise the minimal protein requirements for apo-B mRNA editing in vitro. By UV cross-linking and immunoprecipitation, we show that ACF binds to apo-B mRNA in vitro and in vivo. Cross-linking of ACF is not competed by RNAs with mutations in the mooring sequence. Coimmunoprecipitation experiments identified an ACF-apobec-1 complex in transfected cells. Immunodepletion of ACF from rat liver extracts abolished editing activity. The immunoprecipitated complexes contained a functional holoenzyme. Our results support a model of the editing enzyme in which ACF binds to the mooring sequence in apo-B mRNA and docks apobec-1 to deaminate its target cytidine. The fact that ACF is widely expressed in human tissues that lack apobec-1 and apo-B mRNA suggests that ACF may be involved in other RNA editing or RNA processing events.
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Cites
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Cited by
Error-Prone Repair DNA Polymerases in Prokaryotes and Eukaryotes
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Exome-wide association study of plasma lipids in >300,000 individuals
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Role of the Gut in Lipid Homeostasis
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Transcriptome-wide sequencing reveals numerous APOBEC1 mRNA-editing targets in transcript 3′ UTRs
Rosenberg, B. R., Hamilton, C. E., Mwangi, M. M., Dewell, S., & Papavasiliou, F. N. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1975 · Cited by 232
C to U RNA editing mediated by APOBEC1 requires RNA‐binding protein RBM47
Fossat, N., Tourle, K., Radziewic, T., Barratt, K., Liebhold, D., Studdert, J. B., Power, M., Jones, V., Loebel, D. A. F., & Tam, P. P. L. (2014) · The EMBO Reports · doi:10.15252/embr.201438450 · Cited by 111
Metadata
- Authors
- Mehta, A., Kinter, M. T., Sherman, N. E., & Driscoll, D. M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2000-03
- Volume
- 20
- Issue
- 5
- Pages
- 1846-1854
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2140097965
- Cited by
- 257
- References
- 43
- FWCI
- 8.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Mehta, A., Kinter, M. T., Sherman, N. E., & Driscoll, D. M. (2000). Molecular Cloning of Apobec-1 Complementation Factor, a Novel RNA-Binding Protein Involved in the Editing of Apolipoprotein B mRNA. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.20.5.1846-1854.2000
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