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Cited by 13T-cell and B-cell ImmunologyPI3K/AKT/mTOR signaling in cancerNF-κB Signaling Pathways

Distinct Roles of Phosphoinositide-3 Kinase and Phospholipase Cγ2 in B-Cell Receptor-Mediated Signal Transduction

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Dai, Xuezhi, Chen, Yuhong, Schuman, James, Hua, Zichun, Adamson, John W., Wen, Renren, Wang, Demin·2006·Molecular and Cellular Biology
DOI 10.1128/mcb.26.1.88-99.2006PMC PMC1317611
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Abstract

During B-cell receptor (BCR) signaling, phosphoinositide-3 kinase (PI3K) is thought to function upstream of phospholipase Cgamma2 (PLCgamma2). PLCgamma2 deficiency specifically impedes transitional type 2 (T2) to follicular (FO) mature B-cell transition. Here, we demonstrate that PI3K deficiency specifically impaired T2-to-FO mature B-cell transition and marginal zone B-cell development. Furthermore, we investigated the functional relationship between PI3K and PLCgamma2 using PI3K-/-, PLCgamma2-/-, and PI3K-/- PLCgamma2-/- B cells. Interestingly, PLCgamma2 deficiency had no effect on BCR-mediated PI3K activation, whereas PI3K deficiency only partially blocked activation of PLCgamma2. Moreover, whereas PI3K-/- PLCgamma2-/- double deficiency did not affect hematopoiesis, it resulted in embryonic lethality. PI3K-/- PLCgamma2-/- fetal liver cells transplanted into B-cell null JAK3-/- mice failed to restore development of peripheral B cells and failed to progress through early B-cell development at the pro-B- to pre-B-cell transition, a more severe phenotype than was observed with either PI3K or PLCgamma2 single-deficiency B cells. Consistent with this finding, BCR signaling was more severely impaired in the absence of both PI3K and PLCgamma2 genes than in the absence of either one alone. Taken together, these results demonstrate that whereas PI3K functions upstream of PLCgamma2, activation of PLCgamma2 can occur independently of PI3K and that PI3K and PLCgamma2 also have distinct functions in BCR signal transduction.

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  • PLCγ2-deficient and inducible PLCγ2−/− mice lack the ability to respond to antigens.

    “Indeed, PLCγ2-deficient and inducible PLCγ2−/− mice have been widely shown to lack the ability to respond to antigens (24, 26, 27, 28).”

    From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [27]

    The Abstract (page not provided) states that PLCγ2-deficient B cells are unable to respond to antigens. The paper does not establish the same claim for inducible PLCγ2−/− mice, so that part of the citing sentence is unsupported by our copy. (LLM, conf=0.85)

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Cited by

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Authors
Dai, X., Chen, Y., Schuman, J., Hua, Z., Adamson, J. W., Wen, R., & Wang, D.
Affiliations
  • Versiti Blood Center of Wisconsin (US)
  • State Key Laboratory of Pharmaceutical Biotechnology (CN)
  • Medical College of Wisconsin (US)
ORCID
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
2006-01-01
Volume
26
Issue
1
Pages
88-99
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.26.1.88-99.2006
PMC
PMC1317611
OpenAlex
W2122959084
Cited by
13
References
4
Citations by year
2024: 1 · 2021: 1 · 2017: 1 · 2016: 1 · 2013: 1 · 2012: 1
FWCI
0.59
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R01HL073284)
  • National Institute of Allergy and Infectious Diseases (R01AI052327, R01 AI52327)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
phosphatidylinositol 3-kinase, PLCγ2, B cell receptor signaling, B cell development, marginal zone B cells, embryonic lethality, signal transduction
MeSH terms
Animals, B-Lymphocytes, Cell Differentiation, Embryo, Mammalian, Enzyme Activation, Lymphocyte Activation, Mice, Mutant Strains, Receptors, Antigen, B-Cell, Signal Transduction, Gene Deletion, Phosphatidylinositol 3-Kinases, Embryonic Development, Mice, Phospholipase C gamma

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