Study of Long-Distance Functional Interactions between Su(Hw) Insulators That Can Regulate Enhancer-Promoter Communication in Drosophila melanogaster
Open accessAbstract
The Su(Hw) insulator found in the gypsy retrotransposon is the most potent enhancer blocker in Drosophila melanogaster. However, two such insulators in tandem do not prevent enhancer-promoter communication, apparently because of their pairing interaction that results in mutual neutralization. Furthering our studies of the role of insulators in the control of gene expression, here we present a functional analysis of a large set of transgenic constructs with various arrangements of regulatory elements, including two or three insulators. We demonstrate that their interplay can have quite different outcomes depending on the order of and distance between elements. Thus, insulators can interact with each other over considerable distances, across interposed enhancers or promoters and coding sequences, whereby enhancer blocking may be attenuated, cancelled, or restored. Some inferences concerning the possible modes of insulator action are made from collating the new data and the relevant literature, with tentative schemes illustrating the regulatory situations in particular model constructs.
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Contacts between architectural proteins can modulate the spatial organization of the genome, including keeping enhancers close to promoters.
“In model experiments, it was convincingly shown that contacts between these proteins can modulate the spatial organization of the genome, including keeping enhancers close to promoters [84,91,92].”
From Enhancer Function in the 3D Genome (2023) · cites this paper as [92]
The Discussion describes Su(Hw) insulator pairing as changing whether an enhancer can approach or communicate with a promoter, and proposes that one arrangement may bring them closer. But the paper says the actual positioning of that arrangement is unknown; its experiments establish functional effects, not that protein contacts were shown to keep enhancers physically close to promoters. (LLM, conf=0.83)
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Cites
Looping and Interaction between Hypersensitive Sites in the Active β-globin Locus
Tolhuis, B., Palstra, R.-J., Splinter, E., Grosveld, F., & de Laat, W. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00781-5 · Cited by 1,344 · see citing works
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Research works printed but not linked: 6
- [8]8. Burgess-Beusse, B., C. Farrell, M. Gaszner, M. Litt, V. Mutskov, F. RecillasTarga, M. Simpson, A. West, and G. Felsenfeld. 2002. The insulation of genes from external enhancers and silencing chromatin. Proc. Natl. Acad. Sci. USA 99: 16433-16437.
- [42]42. Lindsley, D. L., and G. G. Zimm. 1992. The genome of Drosophila melanogaster. Academic Press, New York, N.Y.
- [43]43. Majumder, P., and H. N. Cai. 2003. The functional analysis of insulator interactions in the Drosophila embryo. Proc. Natl. Acad. Sci. USA 100: 52235228.
- [51]51. Parnell, T. J., M. M. Viering, A. Skjesol, C. Helou, E. J. Kuhn, and P. K. Geyer. 2003. An endogenous Suppressor of Hairy-wing insulator separates regulatory domains in Drosophila . Proc. Natl. Acad. Sci. USA 100: 1343613441.
- [53]53. Recillas-Targa, F., A. C. Bell, and G. Felsenfeld. 1999. Positional enhancerblocking activity of the chicken beta-globin insulator in transiently transfected cells. Proc. Natl. Acad. Sci. USA 96: 14354-14359.
- [66]66. Wei, W., and M. D. Brennan. 2000. Polarity of transcriptional enhancement revealed by an insulator element. Proc. Natl. Acad. Sci. USA 97: 1451814523.
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Chromatin loops as allosteric modulators of enhancer-promoter interactions
Doyle, B., Fudenberg, G., Imakaev, M., & Mirny, L. A. (2014) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1003867 · Cited by 114 · see citing works
“(B) A recent study in Drosophila suggested a 7 kb chromatin loop formed between Su(Hw) insulators (orange) could decrease E-P interactions (red ‘‘X’’) [20].”
Enhancer Function in the 3D Genome
Razin, S. V., Ulianov, S. V., & Iarovaia, O. V. (2023) · Genes · doi:10.3390/genes14061277 · Cited by 15 · see citing works
- Authors
- Savitskaya, E., Melnikova, L., Kostuchenko, M., Kravchenko, E., Pomerantseva, E., Boikova, T., Chetverina, D., Parshikov, A., Zobacheva, P., Gracheva, E., Galkin, A., & Georgiev, P.
- Affiliations
- Russian Academy of Sciences (RU)
- Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences
- Institute of Agricultural Biotechnology
- Corresponding author
- Georgiev, Pavel
- ORCID
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2006-02
- Volume
- 26
- Issue
- 3
- Pages
- 754-761
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC1347022
- OpenAlex
- W2148588894
- Cited by
- 52
- References
- 1
- Citations by year
- 2024: 4 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 5 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 1.77
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- gypsy retrotransposon, enhancer-promoter interactions, enhancer blocking, Drosophila melanogaster, gene expression regulation, chromatin looping
- MeSH terms
- Animals, Drosophila melanogaster, Enhancer Elements, Genetic, Gene Expression Regulation, Promoter Regions, Genetic, Retroelements, Insulator Elements
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