The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
Open accessAbstract
Monoclonal antibodies were prepared against a fraction of nuclear proteins of Drosophila melanogaster identified as tightly binding to DNA. Four of these antibodies were directed against a 19-kilodalton nuclear protein; immunofluorescence staining of the polytene chromosomes localized the antigen to the alpha, beta, and intercalary heterochromatic regions. Screening of a lambda gt11 cDNA expression library with one of the monoclonal antibodies identified a recombinant DNA phage clone that produced a fusion protein immunologically similar to the heterochromatin-associated protein. Polyclonal sera directed against the bacterial lacZ fusion protein recognized the same nuclear protein on Western blots. A full-length cDNA clone was isolated from a lambda gt10 library, and its DNA sequence was obtained. Analysis of the open reading frame revealed an 18,101-dalton protein encoded by this cDNA. Two overlapping genomic DNA clones were isolated from a Charon 4 library of D. melanogaster with the cDNA clone, and a restriction map was obtained. In situ hybridization with these probes indicated that the gene maps to a single chromosome location at 29A on the 2L chromosome. This general strategy should be effective for cloning the genes and identifying the genetic loci of chromosomal proteins which cannot be readily assayed by other means.
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“Histone H3K9me3 serves as a binding substrate for heterochromatin protein 1 (HP1) [9–12]”
From Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling (2014) · cites this paper as [9]
In the citing paper
“Histone H3K9me3 serves as a binding substrate for heterochromatin protein 1 (HP1) [9–12]”
From Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling (2014) · cites this paper as [12]
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Shilatifard, A. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.75.103004.142422 · Cited by 1,148
Structure and Function in the Nucleus
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Functional Coherence of the Human Y Chromosome
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Chromatin structure and the inheritance of epigenetic information
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Metadata
- Authors
- James, T. C., & Elgin, S. C.
- ORCID
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1986-11
- Volume
- 6
- Issue
- 11
- Pages
- 3862-3872
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC367149
- OpenAlex
- W2101092089
- Cited by
- 643
- References
- 58
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 21 · 2023: 7 · 2022: 13 · 2021: 37 · 2020: 16 · 2019: 13 · 2018: 12 · 2017: 10 · 2016: 12 · 2015: 21 · 2014: 26 · 2013: 14 · 2012: 14
- FWCI
- 2.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Keywords
- non-histone proteins, heterochromatin, Drosophila melanogaster, monoclonal antibodies, polytene chromosomes, cDNA cloning, gene mapping, chromosome 2L, chromatin proteins
- MeSH terms
- Amino Acid Sequence, Animals, Antibodies, Base Sequence, Chromosomal Proteins, Non-Histone, Chromosomes, DNA, Recombinant, Drosophila melanogaster, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Genes, Heterochromatin
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