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Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production.
Open accessAbstract
Retrovirus vectors can be made in the absence of helper virus by using retrovirus packaging cell lines. Helper-free virus is critical for a variety of gene transfer studies. The most useful packaging cell lines contain helper virus DNA from which the signal required for packaging of the viral RNA genome into virions has been deleted. However, we showed that the ability to package virus is conferred at very low frequency to cells infected with virus from these packaging cell lines, presumably by low-frequency transmission of the deleted virus genome. In addition, these packaging cell lines can interact with some retroviral vectors to yield replication-competent virus. We constructed packaging cell lines containing helper virus DNA that had several alterations in addition to deletion of the packaging signal. The new packaging cells retained the useful features of previously available lines but did not yield helper virus after introduction of any of the vectors tested, and transfer of the packaging function was not detected.
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Cited by
Burns, J. C., Friedmann, T., Driever, W., Burrascano, M., & Yee, J. K. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.17.8033 · Cited by 1,399
Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis.
Stenmark, H., Parton, R. G., Steele‐Mortimer, O., Lütcke, A., Gruenberg, J., & Zerial, M. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06381.x · Cited by 968
Miller, D. G., Adam, M. A., & Miller, A. D. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.8.4239 · Cited by 893
Self-citationLineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.
Price, J., Turner, D., & Cepko, C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.1.156 · Cited by 873
Kohn, D. B., Weinberg, K. I., Nolta, J. A., Heiss, L. N., Lenarsky, C., Crooks, G. M., Hanley, M. E., Annett, G., Brooks, J. S., El-Khoureiy, A., et al. (1995) · Nature Medicine · doi:10.1038/nm1095-1017 · Cited by 594
Metadata
- Authors
- Miller, A. D., & Buttimore, C.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1986-08
- Volume
- 6
- Issue
- 8
- Pages
- 2895-2902
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2161545291
- Cited by
- 1,370
- References
- 0
- FWCI
- 24.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Miller, A. D., & Buttimore, C. (1986). Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.6.8.2895
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
