The DOI matches its Crossref record.
Human growth hormone as a reporter gene in regulation studies employing transient gene expression.
Open accessAbstract
The human growth hormone (hGH) transient assay system described here is based on the expression of hGH directed by cells transfected with hGH fusion genes. Levels of secreted hGH in the medium, measured by a simple radioimmunoassay, are proportional to both levels of cytoplasmic hGH mRNA and the amount of transfected DNA. The system is extremely sensitive, easy to perform, and is qualitatively different from other transient expression systems in that the medium is assayed and the cells themselves are not destroyed. The hGH transient assay system is appropriate for analyses of regulation of gene expression and was utilized here to investigate the effect of the simian virus 40 enhancer on the herpes simplex virus thymidine kinase promoter and the effect of zinc on the mouse metallothionein-I promoter. The expression of hGH can also be used as an internal control to monitor transfection efficiency along with any other transient expression system. All cell types tested thus far (including AtT-20, CV-1, GC, GH4, JEG, L, and primary pituitary cells) were able to secrete hGH into the medium.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Shakhov, A. N., Collart, M. A., Vassalli, P., Nedospasov, S. A., & Jongeneel, C. V. (1990) · The Journal of Experimental Medicine · doi:10.1084/jem.171.1.35 · Cited by 819
Hentze, M. W., Caughman, S. W., Rouault, T. A., Barriocanal, J. G., Dancis, A., Harford, J. B., & Klausner, R. D. (1987) · Science · doi:10.1126/science.3685996 · Cited by 571
Rhéaume, E., Lachance, Y., Zhao, H.-F., Breton, N., Dumont, M., Launoit, Y. D., Trudel, C., Luu-The, V., Simard, J., & Labrie, F. (1991) · Molecular Endocrinology · doi:10.1210/mend-5-8-1147 · Cited by 362
Tsai, S. F., Strauss, E., & Orkin, S. H. (1991) · Genes & Development · doi:10.1101/gad.5.6.919 · Cited by 327
Activation of the erythropoietin receptor promoter by transcription factor GATA-1.
Zon, L. I., Youssoufian, H., Mather, C., Lodish, H. F., & Orkin, S. H. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10638 · Cited by 221
Metadata
- Authors
- Selden, R. F., Howie, K. B., Rowe, M. E., Goodman, H. M., & Moore, D. D.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1986-09
- Volume
- 6
- Issue
- 9
- Pages
- 3173-3179
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1954861259
- Cited by
- 663
- References
- 0
- FWCI
- 12.57
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Human growth hormone as a reporter gene in regulation studies employing transient gene expression. https://app.cochat.ai/papers/10.1128/mcb.6.9.3173
- The paper
- Selden, R. F., Howie, K. B., Rowe, M. E., Goodman, H. M., & Moore, D. D. (1986). Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.6.9.3173
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
