The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Transcription Factor E2F Is Required for Efficient Expression of the Hamster Dihydrofolate Reductase Gene In Vitro and In Vivo
Open accessAbstract
The dihydrofolate reductase (DHFR) gene encodes an enzyme important for metabolism and cell growth. We have found multiple DNA-protein interactions within the hamster DHFR gene promoter in vitro. These interactions occur over the consensus binding sites for two eucaryotic transcription factors. Sp1 and E2F. The DHFR E2F consensus site possesses a dyad symmetry and is unique in its location immediately 3' to the major transcription start site. The interaction of E2F with the DHFR promoter has been detected in HeLa nuclear extracts, confirmed by using partially purified E2F, and characterized by both enzymatic and chemical assays of the DNA-protein interaction. A mutation of the E2F recognition sequence which abolishes E2F binding to the DHFR promoter results in a two- to fivefold decrease of in vitro transcriptional activity and a fivefold reduction of DHFR promoter activity in transient-expression assays. Thus, the interaction of E2F with the DHFR promoter is required for efficient expression of the DHFR gene.
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Citations
Cites
Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control.
Kovesdi, I., Reichel, R., & Nevins, J. R. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.8.2180 · Cited by 278
Cited by
Weinberg, R. A. (1991) · Science · doi:10.1126/science.1659741 · Cited by 1,756
The Rb/E2F pathway: expanding roles and emerging paradigms
Harbour, J. W., & Dean, D. C. (2000) · Genes & Development · doi:10.1101/gad.813200 · Cited by 1,153
Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
Kaelin, W. G., Krek, W., Sellers, W. R., DeCaprio, J. A., Ajchenbaum, F., Fuchs, C. S., Chittenden, T., Li, Y., Farnham, P. J., Blanar, M. A., et al. (1992) · Cell · doi:10.1016/0092-8674(92)90108-o · Cited by 819
Tumor Induction and Tissue Atrophy in Mice Lacking E2F-1
Yamasaki, L., Jacks, T., Bronson, R., Goillot, E., Harlow, E., & Dyson, N. J. (1996) · Cell · doi:10.1016/s0092-8674(00)81254-4 · Cited by 698
Retinoblastoma protein switches the E2F site from positive to negative element
Weintraub, S. J., Prater, C. A., & Dean, D. C. (1992) · Nature · doi:10.1038/358259a0 · Cited by 657
Metadata
- Authors
- Blake, M. C., & Azizkhan, J. C.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1989-11-01
- Volume
- 9
- Issue
- 11
- Pages
- 4994-5002
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1846573294
- Cited by
- 287
- References
- 1
- FWCI
- 6.08
- Type
- journal article
- Language
- en
- Open access
- green
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- Blake, M. C., & Azizkhan, J. C. (1989). Transcription Factor E2F Is Required for Efficient Expression of the Hamster Dihydrofolate Reductase Gene In Vitro and In Vivo. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.9.11.4994-5002.1989
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
