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A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli
Open accessAbstract
We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most positions both Tn10 (Tetr) and TN10kan (Kanr) elements are available, so that the collection contains a linked set of alternating antibiotic resistance markers. The map position of each insertion has been aligned to the E. coli genetic map as well as to the Kohara ordered clone bank. These strains are designed to be used in a rapid two-step mapping system in E. coli. In the first step, the mutation is localized to a 5- to 15-min region of the chromosome by Hfr mapping with a set of Hfr strains containing either Tn10 or Tn10kan elements located 20 min from their respective origins of transfer. In the second step, the mutation is localized to a 1-min region by P1 transduction, with a collection of isogenic insertion strains as donors. We discuss the uses of this collection of strains to map and eventually to clone a variety of mutations in E. coli.
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Citations
Cited by
SOS response promotes horizontal dissemination of antibiotic resistance genes
Beaber, J. W., Hochhut, B., & Waldor, M. K. (2004) · Nature · doi:10.1038/nature02241 · Cited by 1,186
SeqA: A negative modulator of replication initiation in E. coli
LU, M. (1994) · Cell · doi:10.1016/0092-8674(94)90156-2 · Cited by 508
Stewart, E. J. (1998) · The EMBO Journal · doi:10.1093/emboj/17.19.5543 · Cited by 432
Adaptation to the fitness costs of antibiotic resistance in Escherichia coli
Schrag, S. J., Perrot, V., & Levin, B. R. (1997) · Proceedings of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rspb.1997.0178 · Cited by 396
Schwartz, C. J., Djaman, O., Imlay, J. A., & Kiley, P. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.160261497 · Cited by 301
Metadata
- Authors
- Singer, M., Baker, T. A., Schnitzler, G. R., Deischel, S. M., Goel, M. C., Dove, W. F., Siranosian, K. J., Grossman, A. D., Erickson, J. W., & Gross, C. A.
- Venue
- Microbiological Reviews
- Publisher
- American Society for Microbiology
- Published
- 1989-01-01
- Volume
- 53
- Issue
- 1
- Pages
- 1-24
- ISSN
- 0146-0749 (ISSN-L)
- OpenAlex
- W1938326461
- Cited by
- 738
- References
- 0
- FWCI
- 21.15
- Type
- journal article
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. https://app.cochat.ai/papers/10.1128/mmbr.53.1.1-24.1989
- The paper
- Singer, M., Baker, T. A., Schnitzler, G. R., Deischel, S. M., Goel, M. C., Dove, W. F., Siranosian, K. J., Grossman, A. D., Erickson, J. W., & Gross, C. A. (1989). A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiological Reviews. https://doi.org/10.1128/mmbr.53.1.1-24.1989
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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