The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
A Probiotic Friend
Open accessAbstract
We read with interest the publication by Nougayrède and coworkers who described that Escherichia coli strain Nissle 1917 (EcN) is mutagenic by means of its expressed colibactin (1). The contribution of this work on the biological activity of colibactin is of high significance and increases our knowledge on this enigmatic toxin. The use of mouse models is an important methodology to study the effect of colibactin in vivo. Nougayrède et al. provided evidence that EcN has genotoxic potential in the models they applied (1).
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“colibactin was then detected in Nissle 1917 supernatants, and cell lines exposed to such supernatants were found to have clear DNA mutations [132].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [132]
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“In germ-free mice administered with Nissle 1917, intestinal epithelial cells showed evidence of DNA damage [132], which was not observed when mice were inoculated with variants of Nissle 1917 carrying mutations in clbA or clbP”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [132]
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Citations
Cites
Mutational signature in colorectal cancer caused by genotoxic pks+ E. coli
Pleguezuelos-Manzano, C., Puschhof, J., Rosendahl Huber, A., van Hoeck, A., Wood, H. M., Nomburg, J., Gurjao, C., Manders, F., Dalmasso, G., Stege, P. B., et al. (2020) · Nature · doi:10.1038/s41586-020-2080-8 · Cited by 1,208
Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
Nougayrède, J.-P., Homburg, S., Taieb, F., Boury, M., Brzuszkiewicz, E., Gottschalk, G., Buchrieser, C., Hacker, J., Dobrindt, U., & Oswald, E. (2006) · Science · doi:10.1126/science.1127059 · Cited by 1,188
Microcins mediate competition among Enterobacteriaceae in the inflamed gut
Sassone-Corsi, M., Nuccio, S.-P., Liu, H., Hernandez, D., Vu, C. T., Takahashi, A. A., Edwards, R. A., & Raffatellu, M. (2016) · Nature · doi:10.1038/nature20557 · Cited by 549
High Prevalence of Mucosa-Associated E. coli Producing Cyclomodulin and Genotoxin in Colon Cancer
Buc, E., Dubois, D., Sauvanet, P., Raisch, J., Delmas, J., Darfeuille-Michaud, A., Pezet, D., & Bonnet, R. (2013) · PLoS ONE · doi:10.1371/journal.pone.0056964 · Cited by 535
Test No. 471: Bacterial Reverse Mutation Test
OECD (2009) · OECD guidelines for the testing of chemicals. Section 4: Health effects · doi:10.1787/9789264071247-en · Cited by 442
Probiotics: Properties, Examples, and Specific ApplicationsPreprint
Behnsen, J., Deriu, E., Sassone‐Corsi, M., & Raffatellu, M. (2013) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a010074 · Cited by 305
Large-Scale Population Structure of Human Commensal Escherichia coli Isolates
Escobar‐Páramo, P., Grenet, K., Menach, A. L., Rode, L., Salgado, E., Amorin, C., Gouriou, S., Picard, B., Rahimy, M. C., Andremont, A., et al. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.9.5698-5700.2004 · Cited by 243
Colon cancer-associated B2Escherichia colicolonize gut mucosa and promote cell proliferation
Raisch, J. (2014) · World Journal of Gastroenterology · doi:10.3748/wjg.v20.i21.6560 · Cited by 189
Test No. 489: In Vivo Mammalian Alkaline Comet Assay
OECD (2016) · OECD guidelines for the testing of chemicals. Section 4: Health effects · doi:10.1787/9789264264885-en · Cited by 158
Escherichia coli Nissle 1917 in Ulcerative Colitis Treatment: Systematic Review and Meta-analysis
Losurdo, G., Iannone, A., Contaldo, A., Ierardi, E., Leo, A. D., & Principi, M. (2015) · Journal of Gastrointestinal and Liver Diseases · doi:10.15403/jgld.2014.1121.244.ecn · Cited by 109
Cited by
Sorted by citations
Hof, M. V. ‘., Mohite, O. S., Monk, J. M., Weber, T., Palsson, B. Ø., & Sommer, M. O. A. (2022) · BMC Bioinformatics · doi:10.1186/s12859-022-05108-9 · Cited by 24
Pierro, F. D., Thacharodi, A., Kumaraswami, M., Suvorov, A. N., Zupet, J., & Zerbinati, N. (2026) · Microbial Cell Factories · doi:10.1186/s12934-026-02997-w · Cited by 8
Di Pierro, F., Sagheddu, V., Galletti, S., Casaroli, A., Labrini, E., Soldi, S., Cazzaniga, M., Bertuccioli, A., Matera, M., Cavecchia, I., et al. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10522-5 · Cited by 5
“In germ-free mice administered with Nissle 1917, intestinal epithelial cells showed evidence of DNA damage [132], which was not observed when mice were inoculated with variants of Nissle 1917 carrying mutations in clbA or clbP , genes encoded within the pks cluster and which are directly involved…”
Regulatory Aspects Relevant to Probiotic Products
Chugh, P., Misra, S., Dhar, M. S., & Raghuwanshi, S. (2023) · Springer eBooks · doi:10.1007/978-981-99-1463-0_25 · Cited by 4
Reply to Dubbert and von Bünau, “A Probiotic Friend”
Nougayrède, J., & Oswald, É. (2021) · mSphere · doi:10.1128/msphere.00906-21 · Cited by 3
Metadata
- Authors
- Dubbert, S., & von Bünau, R.
- Affiliations
- Ardeypharm (Germany) (DE)
- Ardeypharm GmbH, Herdecke, Germany
- Corresponding author
- Dubbert, Silke
- Venue
- mSphere
- Publisher
- American Society for Microbiology
- Published
- 2021-12-22
- Volume
- 6
- Issue
- 6
- Pages
- e0085621-e0085621
- ISSN
- 2379-5042 (ISSN-L)
- PMC
- PMC8694194
- OpenAlex
- W4200252078
- Cited by
- 6
- References
- 17
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mSphere (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- colibactin, Escherichia coli Nissle 1917, probiotics, mutagenicity, genotoxicity, bacterial toxins, mouse model
- MeSH terms
- Humans, Probiotics, Escherichia coli Proteins, Friends
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