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An ultrastructural study of the effect of neomycin on the colon in the human subject and in the conventional and the germ-free mouse
Open accessAbstract
An electron microscopic study of the colon of normal mice and human subjects and those treated with neomycin is reported; there is a close resemblance between the mouse and human colons. After rapid disinfection of the colon, there is epithelial cell damage due to a toxic effect of the drug, a reduction in epithelial turnover accompanying the change in flora, and an important reduction in the cellularity of the lamina propria mainly due to a reduction in inflammatory cells. The changes in the lamina propria probably represent changes in the antipathogenetic defences of the host.
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“GF animals are smaller in body weight and have impaired intestinal function (Aluwihare, 1971;Jeppsson et al., 1979;Savage et al., 1981), have lower concentrations of most gastrointestinal luminal amino acids than SPF mice (Yamamoto et al., 2018), and actually live longer (Gordon et al., 1966b;Reyniers and Sacksteder, 1958;Tazume et al., 1991;Wostman, 1968).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Aluwihare, 1971
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Research works printed but not linked: 27
- Abrams, G. D., Bauer, H., and Sprinz, H. (1963). Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. Lab. Invest., 12, 355-364.
- Aluwihare, A. P. R. (1969). The effect of neomycin on the colon. M.Chir. Thesis, Cambridge.
- Bauer, H., Horowitz, R. E., Levenson, S. M., and Popper, H. (1963). The response of the lymphatic tissue to the microbial flora. Amer. J. Path., 42, 471-483.
- Brotzu, G. (1962). Cytotoxic and cytostatic action of various antibiotics. Minerva chir., 17, 1254-1257.
- Corrin, B., Clarke, A. E., and Spencer, H. (1969). Ultrastructural localization of acid phosphatase in the rat lung. J. Anat. (Lond.), 104, 65-70.
- Davenport, H. A. (1960). Histological and Histochemical Technics. Saunders, Philadelphia and London.
- Deane, H. W. (1964). Some electron microscopic observations on the lamina propria of the gut, with comments on the close association of macrophages, plasma cells, and eosinophils. Anat. Rec., 149, 453-473. · DOI
- Dineen, P. (1961). The effect of neomycin bowel preparation on the susceptibility to systemic Staphylococcal infection. Surgery, 49, 727-730.
- Dobbins, W. O., Herrero, B. A., and Mansbach, C. M. (1968). Morphologic alterations associated with Neomycin induced malabsorption. Amer. J. med. Sci., 255, 63-77.
- Donellan, W. L. (1965). The structure of the colonic mucosa. Gastroenterology, 49, 496-514. · DOI
- Dubos, R. J., and Schaedler, R. W. (1960). The effect of the intestinal flora on the growth rate of mice and on their susceptibility to experimental infections. J. exp. Med., 111, 407-417. · DOI
- Friedmann, I., Dadswell, J. V., and Bird, E. S. (1966). Electronmicroscope studies of the neuroepithelium of the inner ear in guinea-pigs treated with neomycin. J. Path. Bact., 92, 415-422. · DOI
- Hally, A. D. (1964). A counting method for measuring the volume of tissue components in microscopical sections. Quart. J. micr. Sci., 105, 503-514.
- Heneghan, J. B. (1968). Ehrlich ascites tumour growth in gnotobiotic mice. In Advances in Germ-Free Research and Gnotobiology, edited by M. Miyakawa and T. D. Luckey. Iliffe Books, London.
- Kenworthy, R. (1968). Mild physiologic inflammation of conventional life. In Advances in Germ-Free Research and Gnotobiology, edited by M. Miyakawa and T. D. Luckey. Iliffe Books, London.
- Lancet (1970). Editorial The colon and the surgeon. Lancet, 1, 509-510.
- Le Blond, C. P., and Stevens, C. E. (1948). The constant renewal of the intestinal epithelium in the albino rat. Anat. Rec., 100, 357-377. · DOI
- Leon, S. A., and Brock, T. D. (1967). Effect of streptomycin and neomycin on physical properties of the ribosome. J. molec. Biol., 24, 391-404. · DOI
- Nakao, K., and Levenson, S. M. (1967). Atypical mitochondrial morphology of the intestinal absorptive cells of the germ-free rat. Experientia (Basel), 23, 494-496. · DOI
- Pascal, R. R., Kaye, G. I., and Lane, N. (1968). Colonic pericryptal fibroblast sheath I. Gastroenterology, 54, 835-851. · DOI
- Pittman, F. E., and Pittman, J. C. (1966). An electron microscopic study of the epithelium of normal human sigmoid colonic mucosa. Gut, 7, 644-661. · DOI
- Poth, E. J. (1952). Intestinal antisepsis. West. J. Surg., 60, 205-210.
- Rusnyak, I., Foldi, M., and Szabo, G. (1967). Lymphatics and Lymph
- Savage, D. C., and Dubos, R. (1968). Alterations in the mouse cecum and its flora produced by antibacterial drugs. J. exp. Med., 128, 97-110. · DOI
- Sprinz, H., Kundel, D. W., Dammin, G. J., Horowitz, R. E., Schneider, H., and Formal, S. B. (1961). The response of the germ-free guinea pig to oral bacterial challenge with E. coli and Shigella flexneri. Amer. J. Path., 39, 681-695. van der Waaij, D. (1968). Production of bacteria free mice by antibiotic decontamination. In Advances in Germ-Free Research and Gnotobiology, edited by M. Miyakawa and T. D. Luckey. Iliffe Books, London.
- Woodward, S. C., Herrmann, J. B., Shadomy, S., and Pulaski, E. J. (1964). Oral neomycin and the healing of colonic anastomoses in the rat. Surg. Gynec. Obstet., 119, 799-806.
- Zweifach, B. W., Grant, L., and McCluskey, R. T. (1965). The Inflammatory Process. Academic Press, New York.
Cited by
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Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286
Metadata
- Authors
- Aluwihare, A. P. R.
- Affiliations
- Royal College of Surgeons of England (GB)
- Corresponding author
- Aluwihare, A. P. R.
- Venue
- Gut
- Publisher
- BMJ
- Published
- 1971-05
- Volume
- 12
- Issue
- 5
- Pages
- 341-349
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1411602
- OpenAlex
- W2052363747
- Cited by
- 14
- References
- 0
- Citations by year
- 2023: 1 · 2019: 1 · 2018: 1 · 2016: 1 · 2014: 1 · 2013: 2
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
- Keywords
- neomycin, germ-free mice, human colon, epithelial damage, epithelial cell turnover, inflammatory cells, gut microbiota, drug toxicity, host defense against infection
- MeSH terms
- Animals, Basement Membrane, Cecum, Cell Nucleus, Collagen, Colon, Connective Tissue, Epithelial Cells, Epithelium, Fibroblasts, Germ-Free Life, Humans, Intestinal Mucosa, Microscopy, Electron, Neomycin, Nerve Tissue, Mice
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