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Novel genotype–phenotype associations demonstrated by high-throughput sequencing in patients with hypertrophic cardiomyopathy

Lopes, Luis R, Syrris, Petros, Guttmann, Oliver P, O'Mahony, Constantinos, Tang, Hak Chiaw, Dalageorgou, Chrysoula, Jenkins, Sharon, Hubank, Mike, Monserrat, Lorenzo, McKenna, William J, Plagnol, Vincent, Elliott, Perry M·2015·Heart
DOI 10.1136/heartjnl-2014-306387PMC PMC4345808
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Abstract

Objective: A predictable relation between genotype and disease expression is needed in order to use genetic testing for clinical decision-making in hypertrophic cardiomyopathy (HCM). The primary aims of this study were to examine the phenotypes associated with sarcomere protein (SP) gene mutations and test the hypothesis that variation in non-sarcomere genes modifies the phenotype. Methods: Unrelated and consecutive patients were clinically evaluated and prospectively followed in a specialist clinic. High-throughput sequencing was used to analyse 41 genes implicated in inherited cardiac conditions. Variants in SP and non-SP genes were tested for associations with phenotype and survival. Results: 874 patients (49.6±15.4 years, 67.8% men) were studied; likely disease-causing SP gene variants were detected in 383 (43.8%). Patients with SP variants were characterised by younger age and higher prevalence of family history of HCM, family history of sudden cardiac death, asymmetric septal hypertrophy, greater maximum LV wall thickness (all p values<0.0005) and an increased incidence of cardiovascular death (p=0.012). Similar associations were observed for individual SP genes. Patients with ANK2 variants had greater maximum wall thickness (p=0.0005). Associations at a lower level of significance were demonstrated with variation in other non-SP genes. Conclusions: Patients with HCM caused by rare SP variants differ with respect to age at presentation, family history of the disease, morphology and survival from patients without SP variants. Novel associations for SP genes are reported and, for the first time, we demonstrate possible influence of variation in non-SP genes associated with other forms of cardiomyopathy and arrhythmia syndromes on the clinical phenotype of HCM.

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Figure 1 Comparison between sarcomere gene mutation-positive and -negative patients. (A) Age at initial evaluation (45.78±14.65 vs 53.05±14.94 years). (B) Family history of hypertrophic cardiomyopathy (HCM). (C) Family history of sudden cardiac death (SCD). (D) Hypertrophy pattern. (E) Maximum wall thickness (18.83±4.42 vs 18.12±4.08 mm). (F) Implanted implantable cardioverter-defibrillators (ICDs). Key: 0: sarcomere-negative; 1: sarcomere-positive. For B, C, E: red colour and percentages indicate the individuals with the trait within each genotype; for D light blue—asymmetric septal hypertrophy; red—apical hypertrophy; green—concentric hypertrophy.
Figure 1 Comparison between sarcomere gene mutation-positive and -negative patients. (A) Age at initial evaluation (45.78±14.65 vs 53.05±14.94 years). (B) Family history of hypertrophic cardiomyopathy (HCM). (C) Family history of sudden cardiac death (SCD). (D) Hypertrophy pattern. (E) Maximum wall thickness (18.83±4.42 vs 18.12±4.08 mm). (F) Implanted implantable cardioverter-defibrillators (ICDs). Key: 0: sarcomere-negative; 1: sarcomere-positive. For B, C, E: red colour and percentages indicate the individuals with the trait within each genotype; for D light blue—asymmetric septal hypertrophy; red—apical hypertrophy; green—concentric hypertrophy.CC BY · PubMed Central
Figure 2 Kaplan–Meier cumulative incidence curves for cardiovascular death (see Methods section), comparing sarcomere-positive and sarcomere-negative individuals, modelled for (A): follow-up from first evaluation (years), log-rank test p value=0.012 (HR 2.81; 95% CI 1.21 to 6.51) and (B): time from birth (years), log-rank test p value=0.001 (HR 3.99; 95% CI 1.71 to 9.36). The Y axis values indicate proportions.
Figure 2 Kaplan–Meier cumulative incidence curves for cardiovascular death (see Methods section), comparing sarcomere-positive and sarcomere-negative individuals, modelled for (A): follow-up from first evaluation (years), log-rank test p value=0.012 (HR 2.81; 95% CI 1.21 to 6.51) and (B): time from birth (years), log-rank test p value=0.001 (HR 3.99; 95% CI 1.71 to 9.36). The Y axis values indicate proportions.CC BY · PubMed Central
Figure 3 Kaplan–Meier cumulative incidence curves for sudden cardiac death/aborted sudden cardiac death, comparing sarcomere-positive and sarcomere-negative individuals, modelled for (A): follow-up from first evaluation (years), log-rank test p value=0.039 (HR 2.89; 95% CI 1.01 to 8.33) and (B): time from birth (years), log-rank test p value=0.028 (HR 3.44; 95% CI 1.19 to 9.92) . The Y axis values indicate proportions.
Figure 3 Kaplan–Meier cumulative incidence curves for sudden cardiac death/aborted sudden cardiac death, comparing sarcomere-positive and sarcomere-negative individuals, modelled for (A): follow-up from first evaluation (years), log-rank test p value=0.039 (HR 2.89; 95% CI 1.01 to 8.33) and (B): time from birth (years), log-rank test p value=0.028 (HR 3.44; 95% CI 1.19 to 9.92) . The Y axis values indicate proportions.CC BY · PubMed Central

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Metadata

Authors
Lopes, L. R., Syrris, P., Guttmann, O. P., O'Mahony, C., Tang, H. C., Dalageorgou, C., Jenkins, S., Hubank, M., Monserrat, L., McKenna, W. J., Plagnol, V., & Elliott, P. M.
Venue
Heart
Publisher
BMJ
Published
2014-10-28
Volume
101
Issue
4
Pages
294-301
ISSN
  • 1355-6037
  • 1468-201X
DOI
10.1136/heartjnl-2014-306387
PMC
PMC4345808
Cited by
162
References
0
Language
en

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