The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction.
Open accessAbstract
Several DNA extraction techniques were quantitatively and qualitatively compared using both fresh and paraffin wax embedded tissue and their suitability investigated for providing DNA and RNA for the polymerase chain reaction (PCR). A one hour incubation with proteinase K was the most efficient DNA extraction procedure for fresh tissue. For paraffin wax embedded tissue a five day incubation with proteinase K was required to produce good yields of DNA. Incubation with sodium dodecyl sulphate produced very poor yields, while boiling produced 20% as much DNA as long enzyme digestion. DNA extracted by these methods was suitable for the PCR amplification of a single copy gene. Proteinase K digestion also produced considerable amounts of RNA which has previously been shown to be suitable for PCR analysis. A delay before fixation had no effect on the amount of DNA obtained while fixation in Carnoy's reagent results in a much better preservation of DNA than formalin fixation, allowing greater yields to be extracted.
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Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
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Purification of DNA from formaldehyde fixed and paraffin embedded human tissue
Goelz, S. E., Hamilton, S. R., & Vogelstein, B. (1985) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(85)90390-0 · Cited by 803
An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA Sequences
Kogan, S. C., Doherty, M., & Gitschier, J. (1987) · New England Journal of Medicine · doi:10.1056/nejm198710153171603 · Cited by 797
SIMPLE NON-INVASIVE METHOD TO OBTAIN DNA FOR GENE ANALYSIS
LENCH, N. (1988) · The Lancet · doi:10.1016/s0140-6736(88)92178-2 · Cited by 310
Southern blot analysis of DNA extracted from formalin-fixed pathology specimens.
Dubeau, L., Chandler, L. A., Gralow, J. R., Nichols, P. W., & Jones, P. A. (1986) · PubMed · Cited by 241
The Theory and Practice of Histological Techniques
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Detection of latent virus mRNA in tissues using the polymerase chain reaction
Lynas, C., Cook, S. D., Laycock, K. A., Bradfield, J. W. B., & Maitland, N. J. (1989) · The Journal of Pathology · doi:10.1002/path.1711570404 · Cited by 47
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Mutations in the Rod Domains of Keratins 1 and 10 in Epidermolytic Hyperkeratosis
Rothnagel, J. A., Dominey, A. M., Dempsey, L. D., Longley, M. A., Greenhalgh, D. A., Gagne, T. A., Huber, M., Frenk, E., Hohl, D., & Roop, D. R. (1992) · Science · doi:10.1126/science.257.5073.1128 · Cited by 368
Viral Myocarditis: From Experimental Models to Diagnosis in Patients
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Metadata
- Authors
- Jackson, D. P., Lewis, F. A., Taylor, G. R., Boylston, A. W., & Quirke, P.
- Venue
- Journal of Clinical Pathology
- Publisher
- BMJ
- Published
- 1990-06
- Volume
- 43
- Issue
- 6
- Pages
- 499-504
- ISSN
- 0021-9746 (ISSN-L)
- 1472-4146
- OpenAlex
- W2004831310
- Cited by
- 276
- References
- 9
- FWCI
- 6.35
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jackson, D. P., Lewis, F. A., Taylor, G. R., Boylston, A. W., & Quirke, P. (1990). Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. Journal of Clinical Pathology. https://doi.org/10.1136/jcp.43.6.499
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
