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Cited by 143Chronic Obstructive Pulmonary Disease (COPD) ResearchExercise and Physiological ResponsesMuscle Physiology and Disorders

Hypoxaemia Enhances Peripheral Muscle Oxidative Stress in Chronic Obstructive Pulmonary Disease

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Koechlin, C·2005·Thorax
DOI 10.1136/thx.2004.037531PMC PMC1747208
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Abstract

BACKGROUND: Because oxidative stress affects muscle function, the underlying mechanism to explain exercise induced peripheral muscle oxidative stress in patients with chronic obstructive pulmonary disease (COPD) is clinically relevant. This study investigated whether chronic hypoxaemia in COPD worsens peripheral muscle oxidative stress and whether an abnormal muscle inflammatory process is associated with it. METHODS: Nine chronically hypoxaemic and nine non-hypoxaemic patients performed repeated knee extensions until exhaustion. Biopsy specimens were taken from the vastus lateralis muscle before and 48 hours after exercise. Muscle oxidative stress was evaluated by lipid peroxidation (lipofuscin and thiobarbituric acid reactive substances (TBARs)) and oxidised proteins. Inflammation was evaluated by quantifying muscle neutrophil and tumour necrosis factor (TNF)-alpha levels. RESULTS: When both groups were taken together, arterial oxygen pressure was positively correlated with quadriceps endurance time (n = 18, r = 0.57; p < 0.05). At rest, quadriceps lipofuscin inclusions were significantly greater in hypoxaemic patients than in non-hypoxaemic patients (2.9 (0.2) v 2.0 (0.3) inclusions/fibre; p < 0.05). Exercise induced a greater increase in muscle TBARs and oxidised proteins in hypoxaemic patients than in non-hypoxaemic patients (40.6 (9.1)% v 10.1 (5.8)% and 51.2 (11.9)% v 3.7 (12.2)%, respectively, both p = 0.01). Neutrophil levels were significantly higher in hypoxaemic patients than in non-hypoxaemic patients (53.1 (11.6) v 21.5 (11.2) counts per fibre x 10(-3); p < 0.05). Exercise did not alter muscle neutrophil levels in either group. Muscle TNF-alpha was not detected at baseline or after exercise. CONCLUSION: Chronic hypoxaemia was associated with lower quadriceps endurance time and worsened muscle oxidative stress at rest and after exercise. Increased muscle neutrophil levels could be a source of the increased baseline oxidative damage. The involvement of a muscle inflammatory process in the exercise induced oxidative stress of patients with COPD remains to be shown.

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  • In the quadriceps of COPD patients, muscular TNF-α levels have been reported as significantly elevated, decreased, and unaltered, or even undetected at the protein level.

    “with regards to muscular TNF-α, significantly elevated, decreased, and unaltered levels have been reported in the quadriceps of COPD patients [27,28,29], or even undetected at the protein level [30,31]”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [30]

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Authors
Koechlin, C.
Venue
Thorax
Publisher
BMJ
Published
2005-10-01
Volume
60
Issue
10
Pages
834-841
ISSN
0040-6376 (ISSN-L)
DOI
10.1136/thx.2004.037531
PMC
PMC1747208
OpenAlex
W2142817780
Cited by
143
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 8 · 2019: 2 · 2018: 4 · 2017: 8 · 2016: 10 · 2015: 7 · 2014: 16 · 2013: 10 · 2012: 9
FWCI
10.43
Type
journal article
Language
en
Open access
Locations
  • Thorax (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
Canadian Institutes of Health Research
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
chronic obstructive pulmonary disease, hypoxemia, exercise-induced oxidative stress, lipid peroxidation, lipofuscin, muscle inflammation, vastus lateralis, TNF-α
MeSH terms
Aged, Hypoxia, Anthropometry, Biopsy, Chronic Disease, Forced Expiratory Volume, Humans, Vital Capacity, Biomarkers, Exercise, Oxidative Stress, Muscle, Skeletal, Pulmonary Disease, Chronic Obstructive

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