Hydrogen peroxide-induced oxidative stress, acetylcholinesterase inhibition, and mediated brain injury attenuated by Thymus algeriensis
Open accessAbstract
The aim of the current study was to evaluate acetylcholinesterase (AChE) inhibition, antioxidant enzyme activities, and malondialdehyde (MDA) levels induced by hydrophobic fractions of Thymus algeriensis (HFTS) growing in Tunisia. The results showed that hydrogen peroxide (H 2 O 2), an oxidative stress inducer, acts by decreasing the body mass and brain mass of rats. Moreover, we found higher MDA levels in the group treated with H 2 O 2 (P < 0.05) and a significantly lower activity of catalase, glutathione peroxidase, glutathione S-transferase, and superoxide dismutase, as well as a reduction in reduced glutathione activity in the brain tissues of H 2 O 2-treated rats when compared with those of the control group (P < 0.05); however, rats that received HFTS with H 2 O 2 experienced a decrease in MDA levels in the brain. In contrast, HFTS demonstrated neuroprotective effects in rat brain. Overall, exposure to HFTS prior to H 2 O 2 induced a marked dose-dependent increase in reactive oxygen species scavenger levels (P < 0.05) accompanied by a statistically significant decrease in MDA levels (P < 0.05) when compared with no exposure. Notably, the activity of AChE was affected by exposure to natural compounds; levels were significantly lower in HFTS-treated rats and in those treated with the combination of HFTS and a low or high dose of H 2 O 2. Furthermore, histopathological analysis showed that brain injuries occurred with high doses of H 2 O 2 administered alone or with a low dose of HFTS, whereas a high dose of essential oil markedly alleviated neurone degeneration. The results suggest that HFTS alleviates neuroinflammation by acting as an AChE inhibitor and attenuates H 2 O 2-induced brain toxicity.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Thymus algeriensis hydrophobic fractions (180 mg/kg/day oral) significantly reduced elevated ROS and AChE levels, alleviating neuronal degeneration and restoring AChE activity to near control levels (p<0.05) in H2O2-treated rats.
“oral administration of hydrophobic fractions from Thymus algeriensis (180 mg/kg/day) significantly reduced elevated ROS and AChE levels, alleviating neuronal degeneration and restoring AChE activity to near control levels (p < 0.05) [87].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [87]
The Experimental procedures specify oral HFTS at 180 mg/kg/day, and the AChE inhibition section reports that pretreatment reversed elevated AChE markers toward control values (P<0.05). The Brain Histopathology section reports reduced H2O2-induced neuronal damage. However, the paper reports changes in ROS scavengers and the oxidative-stress marker MDA, not a measured reduction in ROS levels themselves. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 53 · Cited by 27
Research works printed but not linked: 6
- [4]4. Coulibaly, A.Y., Sombie, P.A.E.D., Tibiri, A., Kiendrebeogo, M., Compaore, M.M.Y., and Nacoulma, O.G. 2011. Protective effect of Scoparia dulcis on brain and erythrocytes. Curr. Res. J. Biol. Sci. 3(3): 254-261.
- [6]6. Dastmalchi, K., Dorman, H.J.D., Viorela, H., Hiltunen, R. 2007. Plants as potential source for drug development against Alzheimer's disease. Int. J. Biomed. Pharmaceut. Sci. 1(2): 83-104.
- [17]17. Habig, W.H., Pabst, M.J., Jakoby, W.B. 1974. Glutathione s-transferase: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-9. PMID: 4436300.
- [30]30. Lowry, O.H., Rosenberg, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with of Folin phenol reagents. J. Biol. Chem. 192:265-275. PMID: 14907713.
- [31]31. Marklund, S.L. 1985. Pyrogallol autooxidation. In: Greenwald RA, ed. Handbook of Methods for Oxygen Radical Research. Boca Raton, Florida: CRC Press, 243-7.
- [55]55. Xu, Y., Bradham, C., Brenner, D.A., and Czaja, M.J. 1997. Hydrogen peroxide-induced liver cell necrosis is dependent on AP-1 activation. Am. J. Physiol. 273 (4 Pt 1): G795-803. PMID: 9357820.
- Authors
- Guesmi, F., Bellamine, H., & Landoulsi, A.
- Affiliations
- University of Carthage (TN)
- Service of Anatomo-Pathology of Menzel Bourguiba, Bizerte, Tunisia.
- Venue
- Applied Physiology, Nutrition, and Metabolism
- Publisher
- Canadian Science Publishing
- Published
- 2018-12
- Volume
- 43
- Issue
- 12
- Pages
- 1275-1281
- ISSN
- 1715-5312 (ISSN-L)
- 1715-5320
- OpenAlex
- W2803553180
- Cited by
- 27
- References
- 53
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 6 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 1
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Applied Physiology Nutrition and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (acceptedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- Thymus algeriensis, hydrogen peroxide, oxidative stress, acetylcholinesterase inhibition, neuroprotection, brain injury, antioxidant enzymes, malondialdehyde, essential oils, neuroinflammation, reactive oxygen species scavenging, glutathione, catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, neurodegeneration
- MeSH terms
- Acetylcholinesterase, Animals, Antioxidants, Brain, Cholinesterase Inhibitors, Hydrogen Peroxide, Male, Plant Extracts, Rats, Sprague-Dawley, Oxidative Stress, Thymus Plant, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
