The DOI matches its Crossref record.
A method for the extraction of chlorophyll from leaf tissue without maceration
Abstract
A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.
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Cited by
Sairam, R. K., Deshmukh, P. S., & Shukla, D. S. (1997) · Journal of Agronomy and Crop Science · doi:10.1111/j.1439-037x.1997.tb00486.x · Cited by 490
Physiological recovery of Kentucky bluegrass from simultaneous drought and heat stress
Wang, Z., & Huang, B. (2004) · Crop Science · doi:10.2135/cropsci2004.1729 · Cited by 229
Kapoor, R., Chaudhary, V., & Bhatnagar, A. K. (2007) · Mycorrhiza · doi:10.1007/s00572-007-0135-4 · Cited by 211
Zubair, M., Adnan Ramzani, P. M., Rasool, B., Khan, M. A., ur-Rahman, M., Akhtar, I., Turan, V., Tauqeer, H. M., Farhad, M., Khan, S. A., et al. (2021) · Journal of Environmental Management · doi:10.1016/j.jenvman.2021.112047 · Cited by 174
Metadata
- Authors
- Hiscox, J. D., & Israelstam, G. F.
- Venue
- Canadian Journal of Botany
- Publisher
- Canadian Science Publishing
- Published
- 1979-06-15
- Volume
- 57
- Issue
- 12
- Pages
- 1332-1334
- ISSN
- 0008-4026 (ISSN-L)
- OpenAlex
- W1995452633
- Cited by
- 3,473
- References
- 0
- FWCI
- 3.72
- Type
- journal article
- Language
- fr
- Open access
- closed
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- CoChat. (2026). Citation check: A method for the extraction of chlorophyll from leaf tissue without maceration. https://app.cochat.ai/papers/10.1139/b79-163
- The paper
- Hiscox, J. D., & Israelstam, G. F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany. https://doi.org/10.1139/b79-163
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
