The DOI matches its Crossref record.
A new method for observing the morphology of vesicular–arbuscular mycorrhizae
Open accessAbstract
A new procedure using chlorazol black E has been developed for staining vesicular–arbuscular mycorrhizal fungi in cleared roots. In a comparative study, chlorazol black E was found to be much superior to previously used stains (acid fuchsin, trypan blue, aniline blue) for showing details of internal hyphae and particularly arbuscules. This clearing and staining procedure, combined with Nomarski interference contrast microscopy, revealed details of arbuscule structure not evident with previous techniques. Field-collected samples were also stained well by this procedure. The procedure should allow more accurate assessment of roots for colonization by vesicular–arbuscular mycorrhizal fungi.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi
Vierheilig, H., Coughlan, A. P., Wyss, U., & Piché, Y. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.12.5004-5007.1998 · Cited by 1,648
VARIATION IN PLANT RESPONSE TO NATIVE AND EXOTIC ARBUSCULAR MYCORRHIZAL FUNGI
Klironomos, J. N. (2003) · Ecology · doi:10.1890/02-0413 · Cited by 1,213
Invasive Plant Suppresses the Growth of Native Tree Seedlings by Disrupting Belowground Mutualisms
Stinson, K. A., Campbell, S. A., Powell, J. R., Wolfe, B. E., Callaway, R. M., Thelen, G. C., Hallett, S. G., Prati, D., & Klironomos, J. N. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040140 · Cited by 826
“An approximately 1-g subsample of roots from each seedling was extracted, stained with Chlorazol Black E [37] and analyzed for percent colonization by AMF [38].”
MethodPaszkowski, U., Kroken, S., Roux, C., & Briggs, S. P. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.202474599 · Cited by 688
Journet, E.-P., El-Gachtouli, N., Vernoud, V., de Billy, F., Pichon, M., Dedieu, A., Arnould, C., Morandi, D., Barker, D. G., & Gianinazzi-Pearson, V. (2001) · Molecular Plant-Microbe Interactions® · doi:10.1094/mpmi.2001.14.6.737 · Cited by 299
Metadata
- Authors
- Brundrett, M. C., Piché, Y., & Peterson, R. L.
- Venue
- Canadian Journal of Botany
- Publisher
- Canadian Science Publishing
- Published
- 1984-10-01
- Volume
- 62
- Issue
- 10
- Pages
- 2128-2134
- ISSN
- 0008-4026 (ISSN-L)
- OpenAlex
- W2077972236
- Cited by
- 526
- References
- 0
- FWCI
- 6.35
- Type
- journal article
- Language
- fr
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A new method for observing the morphology of vesicular–arbuscular mycorrhizae. https://app.cochat.ai/papers/10.1139/b84-290
- The paper
- Brundrett, M. C., Piché, Y., & Peterson, R. L. (1984). A new method for observing the morphology of vesicular–arbuscular mycorrhizae. Canadian Journal of Botany. https://doi.org/10.1139/b84-290
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
