The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis
Open accessAbstract
Giardia intestinalis is the most common infectious protozoan parasite in children. Despite the effectiveness of some drugs, the disease remains a major worldwide problem. Consequently, the search for new treatments is important for disease eradication. Biological molecules with antimicrobial properties represent a promising alternative to combat pathogens. Bovine lactoferrin (bLF) is a key component of the innate host defense system, and its peptides have exhibited strong antimicrobial activity. Based on these properties, we evaluated the parasiticidal activity of these peptides on G. intestinalis. Trophozoites were incubated with different peptide concentrations for different periods of time, and the growth or viability was determined by carboxyfluorescein-succinimidyl-diacetate-ester (CFDA) and propidium iodide (PI) staining. Endocytosis of peptides was investigated by confocal microscopy, damage was analyzed by transmission and scanning electron microscopy, and the type of programmed cell death was analyzed by flow cytometry. Our results showed that the LF peptides had giardicidal activity. The LF peptides interacted with G. intestinalis and exposure to LF peptides correlated with an increase in the granularity and vacuolization of the cytoplasm. Additionally, the formation of pores, extensive membrane disruption, and programmed cell death was observed in trophozoites treated with LF peptides. Our results demonstrate that LF peptides exhibit potent in vitro antigiardial activity.
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“In addition, LF is active against the intestinal protozoa Entamoeba histolytica and Giardia lamblia following receptor-mediated endocytosis of its antimicrobial component lactoferricin, that is generated by pepsin-mediated digestion (Aguilar-Diaz et al., 2017;Diaz-Godinez et al., 2019).”
From Iron in infection and immunity (2020) · cites this paper as Aguilar-Diaz et al., 2017
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- Ghosh E., 2009, Drug Des., Dev. Ther., 3, 103
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Iron in infection and immunity
Nairz, M., & Weiss, G. (2020) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2020.100864 · Cited by 359
Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections
Drago-Serrano, M., Campos-Rodríguez, R., Carrero, J., & De la Garza, M. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18030501 · Cited by 151
Self-citationDíaz-Godínez, C., González-Galindo, X., Meza-Menchaca, T., Bobes, R. J., de la Garza, M., León-Sicairos, N., Laclette, J. P., & Carrero, J. C. (2019) · Bioscience Reports · doi:10.1042/bsr20180850 · Cited by 23
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Metadata
- Authors
- Aguilar-Diaz, H., Canizalez-Roman, A., Nepomuceno-Mejia, T., Gallardo-Vera, F., Hornelas-Orozco, Y., Nazmi, K., Bolscher, J. G. M., Carrero, J. C., Leon-Sicairos, C., & Leon-Sicairos, N.
- Affiliations
- CIASaP, Facultad de Medicina, Universidad Autónoma de Sinaloa. Cedros y Sauces, Fracc. Fresnos Culiacán 80246, Sinaloa, México.
- Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Calle 4a, Avenida Norte esquina con Calle 19 Pte S/N, Centro, Tapachula 30700, Chiapas, Mexico.
- Universidad Nacional Autónoma de México (MX)
- Servicio Académico de Microscopía Electrónica de Barrido, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, México, D. F. 04510, México.
- Academic Center for Dentistry Amsterdam (NL)
- Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF 04510, México.
- Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Avenida de las Américas y Josefa Ortiz (Ciudad Universitaria), Culiacán 80030, Sinaloa, México.
- Venue
- Biochemistry and Cell Biology
- Publisher
- Canadian Science Publishing
- Published
- 2017-02
- Volume
- 95
- Issue
- 1
- Pages
- 82-90
- ISSN
- 0829-8211 (ISSN-L)
- 1208-6002
- OpenAlex
- W2558134572
- Cited by
- 35
- References
- 39
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 4 · 2017: 3
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Biochemistry and Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (acceptedVersion)
- UvA-DARE (University of Amsterdam) (submittedVersion)
- VU Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Universiteit van Amsterdam
- Universidad Autónoma de Sinaloa
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Life Sciences › Immunology and Microbiology › Parasitology › Helminth infection and control
- Keywords
- Giardia lamblia, bovine lactoferrin, antigiardial activity, antiparasitic activity, trophozoites, programmed cell death, membrane damage, innate host defense, intestinal protozoa
- MeSH terms
- Animals, Anti-Infective Agents, Cattle, Cell Survival, Feces, Giardiasis, Humans, Lactoferrin, Peptide Fragments, Giardia lamblia, Trophozoites
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- CoChat. (2026). Citation check: Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis. https://app.cochat.ai/papers/10.1139/bcb-2016-0079
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- Aguilar-Diaz, H., Canizalez-Roman, A., Nepomuceno-Mejia, T., Gallardo-Vera, F., Hornelas-Orozco, Y., Nazmi, K., Bolscher, J. G. M., Carrero, J. C., Leon-Sicairos, C., & Leon-Sicairos, N. (2017). Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis. Biochemistry and Cell Biology. https://doi.org/10.1139/bcb-2016-0079
