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A Salicylate–Hypochlorite Method for Determining Ammonia in Seawater
Abstract
Total ammonia-nitrogen (NH 4-N) in artificial seawater was determined spectrophotometrically by a method in which indophenol blue is produced by the reaction of ammonia with salicylate and hypochlorite, in the presence of sodium nitroprusside. Recoveries of 10 to 1000 μg NH 4-N/L by the salicylate– and phenol–hypochlorite methods compared well (r = 0.99); the coefficient of variation for 25 μg NH 4-N/L and above was less than 3% for the salicylate–hypochlorite method. The new method is safer than the phenol–hypochlorite method because phenol is not used, but it may not be suitable for field determinations because of photosensitivity. The characteristic colors produced by increasing concentrations of ammonia make the assay useful for the direct, visual estimation of ammonia in culture systems.Key words: ammonia, analytical methods, seawater analysis
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Citations
Cited by
A Review of Electrocatalytic Reduction of Dinitrogen to Ammonia under Ambient Conditions
Cui, X., Tang, C., & Zhang, Q. (2018) · Advanced Energy Materials · doi:10.1002/aenm.201800369 · Cited by 1,314
Thamdrup, B., & Dalsgaard, T. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.3.1312-1318.2002 · Cited by 1,046
Emerson, D., & Revsbech, N. P. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.11.4022-4031.1994 · Cited by 268
Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery
Dong, H.-P., Williams, E., Wang, D.-Z., Xie, Z.-X., Hsia, R.-C., Jenck, A., Halden, R., Li, J., Chen, F., & Place, A. R. (2013) · Plant Physiology · doi:10.1104/pp.113.214320 · Cited by 173
“Ammonium was determined applying the salicylate-hypochlorite method (Bower and Holm-Hansen, 1980).”
MethodMACY, J. M., NUNAN, K., HAGEN, K. D., DIXON, D. R., HARBOUR, P. J., CAHILL, M., & SLY, L. I. (1996) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-46-4-1153 · Cited by 164
Metadata
- Authors
- Bower, C. E., & Holm-Hansen, T.
- Venue
- Canadian Journal of Fisheries and Aquatic Sciences
- Publisher
- Canadian Science Publishing
- Published
- 1980-05-01
- Volume
- 37
- Issue
- 5
- Pages
- 794-798
- ISSN
- 0706-652X (ISSN-L)
- 1205-7533
- OpenAlex
- W2062720097
- Cited by
- 731
- References
- 0
- FWCI
- 17.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bower, C. E., & Holm-Hansen, T. (1980). A Salicylate–Hypochlorite Method for Determining Ammonia in Seawater. Canadian Journal of Fisheries and Aquatic Sciences. https://doi.org/10.1139/f80-106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
