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Isolation and characterization of Azospirillum mutants excreting high amounts of indoleacetic acid
Abstract
Mutants of Azospirillum brasilense Sp Cd, resistant to 5-fluorotryptophan (FT) excreted 3-indoleacetic acid (IAA), i.e., auxin, producing up to 16 μg/mL which was 30 times greater than the wild-type level. Under conditions of nitrogen fixation, the mutants excreted IAA up to 1 μg/mL, 10 times more than the wild type. However, none of the FT-resistant mutants of Azospirillum lipoferum Sp RG 20a excreted high levels of IAA. This was probably due to differences in the tryptophan and IAA biosynthetic steps between A. brasilense and A. lipoferum strains. Some of the FT-resistant mutants of A. brasilense Sp Cd showed a reduced feedback inhibition of anthranilate synthetase by tryptophan. The increased synthesis of tryptophan could explain the observed excretion of tryptophan and related metabolites. In addition, the IAA-overproducing mutants excreted other amino acids, probably owing to pleiotropic effects of deregulated tryptophan biosynthesis on amino acid metabolism. The growth patterns of some mutants excreting large amounts of IAA were almost identical to those of the wild type.
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Cited by
Indole-3-acetic acid in microbial and microorganism-plant signaling
Spaepen, S., Vanderleyden, J., & Remans, R. (2007) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2007.00072.x · Cited by 1,954
Steenhoudt, O., & Vanderleyden, J. (2000) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(00)00036-x · Cited by 992
Verma, S. (2001) · Journal of Biotechnology · doi:10.1016/s0168-1656(01)00333-9 · Cited by 506
Azospirillum as a potential inoculant for agriculture
Okon, Y. (1985) · Trends in Biotechnology · doi:10.1016/0167-7799(85)90012-5 · Cited by 360
Everything you must know about Azospirillum and its impact on agriculture and beyond
Cassán, F., Coniglio, A., López, G., Molina, R., Nievas, S., de Carlan, C. L. N., Donadio, F., Torres, D., Rosas, S., Pedrosa, F. O., et al. (2020) · Biology and Fertility of Soils · doi:10.1007/s00374-020-01463-y · Cited by 336
Metadata
- Authors
- Hartmann, A., Singh, M., & Klingmüller, W.
- Venue
- Canadian Journal of Microbiology
- Publisher
- Canadian Science Publishing
- Published
- 1983-08-01
- Volume
- 29
- Issue
- 8
- Pages
- 916-923
- ISSN
- 0008-4166 (ISSN-L)
- 1480-3275
- OpenAlex
- W1965640878
- Cited by
- 223
- References
- 0
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hartmann, A., Singh, M., & Klingmüller, W. (1983). Isolation and characterization of Azospirillum mutants excreting high amounts of indoleacetic acid. Canadian Journal of Microbiology. https://doi.org/10.1139/m83-147
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