A novel procedure for rapid isolation of plant growth promoting pseudomonads

Abstract

A rapid and novel procedure for the isolation of plant growth promoting rhizobacteria (PGPR) is described. This method entails screening soil bacteria for the ability to utilize the compound 1-aminocyclopropane-1-carboxylate (ACC) as a sole N source, a trait that is a consequence of the presence of the activity of the enzyme ACC deaminase. This trait appears to be limited to soil bacteria that are also capable of stimulating plant growth. Seven different soil samples from two geographically disparate locations were found to contain pseudomonads that were able to to utilize ACC as a N source. Each of the seven strains was shown, by the ability of the bacterium to promote canola seedling root elongation under gnotobiotic conditions, to be a PGPR. The method described here may be used to replace the otherwise slow and tedious process of testing individual bacterial strains for their ability to promote plant growth, thereby significantly speeding up the process of finding new PGPR.Key words: plant growth promoting rhizobacteria, PGPR, 1-aminocyclopropane-1-carboxylate, ACC, ACC deaminase, bacterial fertilizer, soil bacteria.