Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 17,801–17,900 of 48,846

A Rare Phaeodactylum tricornutum Cruciform Morphotype: Culture Conditions, Transformation and Unique Fatty Acid Characteristics
He, L., Han, X., & Yu, Z. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093922 · Cited by 35
A metabolomic study of preterm and term human and formula milk by proton MRS analysis: preliminary results
Longini, M., Tataranno, M. L., Proietti, F., et al. (2014) · The Journal of Maternal-Fetal & Neonatal Medicine · doi:10.3109/14767058.2014.955958 · Cited by 35
Cyclodextrins differentially mobilize free and esterified cholesterol from primary human foam cell macrophages
Liu, S. M., Cogny, A., Kockx, M., Dean, R. T., Gaus, K., Jessup, W., & Kritharides, L. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200464-jlr200 · Cited by 35
Evaluation of direct transesterification of microalgae using microwave irradiation
Teo, C. L., & Idris, A. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.10.035 · Cited by 35
SCD1 inhibition during 3T3-L1 adipocyte differentiation remodels triacylglycerol, diacylglycerol and phospholipid fatty acid composition
Ralston, J. C., & Mutch, D. M. (2015) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/j.plefa.2015.04.008 · Cited by 35
Impact of future climatic conditions on the potential for soil organic matter priming
Reinsch, S., Ambus, P., Thornton, B., & Paterson, E. (2013) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2013.05.013 · Cited by 37
The soil microbial community response when plants are subjected to water stress and defoliation disturbance
Williamson, W. M., & Wardle, D. A. (2007) · Applied Soil Ecology · doi:10.1016/j.apsoil.2007.05.003 · Cited by 36
A DSC study of phase transition in muscle and oil of the main commercial fish species from the North-Atlantic
Tolstorebrov, I., Eikevik, T. M., & Bantle, M. (2013) · Food Research International · doi:10.1016/j.foodres.2013.11.026 · Cited by 35
Ezetimibe Inhibits Hepatic Niemann-Pick C1-Like 1 to Facilitate Macrophage Reverse Cholesterol Transport in Mice
Xie, P., Jia, L., Ma, Y., Ou, J., Miao, H., Wang, N., Guo, F., Yazdanyar, A., Jiang, X.-C., & Yu, L. (2013) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.112.301187 · Cited by 34
Growth Temperature and Salinity Impact Fatty Acid Composition and Degree of Unsaturation in Peanut‐Nodulating Rhizobia
Paulucci, N. S., Medeot, D. B., Dardanelli, M. S., et al. (2011) · Lipids · doi:10.1007/s11745-011-3545-1 · Cited by 35
Extraction from striped weakfish (Cynoscion striatus) wastes with pressurized CO2: Global yield, composition, kinetics and cost estimation
Aguiar, A. C., Visentainer, J. V., & Martínez, J. (2012) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2012.07.005 · Cited by 35
Overlapping Roles of the Glucose-Responsive Genes, S14 and S14R, in Hepatic Lipogenesis
Aipoalani, D. L., O’Callaghan, B. L., Mashek, D. G., et al. (2010) · Endocrinology · doi:10.1210/en.2009-1058 · Cited by 35
Nutritional Quality of Beef Produced in Chile from Different Production Systems
Morales, R., Folch, C., Iraira, S., Teuber, N., & Realini, C. E. (2012) · Chilean journal of agricultural research · doi:10.4067/s0718-58392012000100013 · Cited by 35
Evaluation of the Nutritional and Storage Quality of Meatballs Formulated with Bee Pollen
Turhan, S., Yazıcı, F., Sarıcaoğlu, F. T., et al. (2014) · Korean Journal for Food Science of Animal Resources · doi:10.5851/kosfa.2014.34.4.423 · Cited by 35
Potential of freeze-dried worm meal as a replacement for fish meal in trout diet formulaions
Hilton, J. W. (1983) · Aquaculture · doi:10.1016/0044-8486(83)90224-7 · Cited by 35
Trophic magnification of polychlorinated biphenyls and polybrominated diphenyl ethers in an estuarine food web of the Ariake Sea, Japan
Kobayashi, J., Imuta, Y., Komorita, T., et al. (2014) · Chemosphere · doi:10.1016/j.chemosphere.2014.08.066 · Cited by 35
Behavior of oxyfluorfen in soils amended with different sources of organic matter. Effects on soil biology
Gómez, I., Rodríguez-Morgado, B., Parrado, J., et al. (2014) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2014.03.051 · Cited by 35
Pattern of natural 15N abundance in lakeside forest ecosystem affected by cormorant-derived nitrogen
Kameda, K. O., Koba, K., Hobara, S., et al. (2006) · Springer eBooks · doi:10.1007/978-1-4020-5556-0_7 · Cited by 35
Testing caffeic acid as a natural antioxidant in functional fish-fibre restructured products
Sánchez‐Alonso, I., Careche, M., Moreno, P., et al. (2010) · LWT · doi:10.1016/j.lwt.2010.11.018 · Cited by 35
The effect of triclosan on microbial community structure in three soils
Butler, E., Whelan, M. J., Ritz, K., et al. (2012) · Chemosphere · doi:10.1016/j.chemosphere.2012.04.002 · Cited by 35
Special methods for the analysis of ether lipid structure and metabolism in archaea
Koga, Y., & Morii, H. (2005) · Analytical Biochemistry · doi:10.1016/j.ab.2005.04.004 · Cited by 36
Brucella abortus Ornithine Lipids Are Dispensable Outer Membrane Components Devoid of a Marked Pathogen-Associated Molecular Pattern
Palacios-Chaves, L., Conde-Álvarez, R., Gil-Ramírez, Y., Zúñiga-Ripa, A., Calvo, E. B., Chacón‐Díaz, C., Chaves‐Olarte, E., Gorvel, V. A., Gorvel, J., Moreno, E., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016030 · Cited by 36
Consequences of Warming and Resource Quality on the Stoichiometry and Nutrient Cycling of a Stream Shredder
Mas‐Martí, E., Romaní, A. M., & Muñoz, I. (2015) · PLoS ONE · doi:10.1371/journal.pone.0118520 · Cited by 35
Chryseomicrobium imtechense gen. nov., sp. nov., a new member of the family Planococcaceae
Arora, P. K., Chauhan, A., Bhawana, P., et al. (2010) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.023184-0 · Cited by 35
Association between polyunsaturated fatty acid-derived oxylipid biosynthesis and leukocyte inflammatory marker expression in periparturient dairy cows
Raphael, W., Halbert, L. W., Contreras, G. A., & Sordillo, L. M. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7656 · Cited by 36
Agrobacteria lacking ornithine lipids induce more rapid tumour formation
Vences‐Guzmán, M. Á., Guan, Z., Bermúdez‐Barrientos, J. R., et al. (2012) · Environmental Microbiology · doi:10.1111/j.1462-2920.2012.02867.x · Cited by 35
Biophysical interaction between corticosteroids and natural surfactant preparation: implications for pulmonary drug delivery using surfactant as a carrier
Wang, Y. E., Zhang, H., Fan, Q., et al. (2011) · Soft Matter · doi:10.1039/c1sm06444d · Cited by 35
Establishment of a Structure–Activity Relationship of 1H-Imidazo[4,5-c]quinoline-Based Kinase Inhibitor NVP-BEZ235 as a Lead for African Sleeping Sickness
Seixas, J. D., Luengo-Arratta, S. A., Díaz-González, R., et al. (2014) · Journal of Medicinal Chemistry · doi:10.1021/jm500361r · Cited by 35
Relationship Between Production of the Phytotoxin Prehelminthosporol and Virulence in Isolates of the Plant Pathogenic Fungus Bipolaris sorokiniana
Apoga, D., Åkesson, H., Jansson, H., et al. (2002) · European Journal of Plant Pathology · doi:10.1023/a:1019976403391 · Cited by 35
Lipids under stress – a lipidomic approach for the study of mood disorders
Miranda, A. M., & Oliveira, T. G. (2015) · BioEssays · doi:10.1002/bies.201500070 · Cited by 38
PCB and PBDE levels in a highly threatened dolphin species from the Southeastern Brazilian coast
Lavandier, R. C., Arêas, J., Quinete, N., et al. (2015) · Environmental Pollution · doi:10.1016/j.envpol.2015.10.013 · Cited by 35
A platform for the structural characterization of glycans enzymatically released from glycosphingolipids extracted from tissue and cells
Anugraham, M., Everest‐Dass, A., Jacob, F., et al. (2015) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.7130 · Cited by 35
Seminal PDC-109 protein vis-à-vis cholesterol content and freezability of buffalo Spermatozoa
Singh, M., Ghosh, S. K., Prasad, J. K., et al. (2013) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2013.10.016 · Cited by 35
Strain Design of Ashbya gossypii for Single-Cell Oil Production
Ledesma‐Amaro, R., Santos, M. Á., Jiménez, A., & Revuelta, J. L. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.03560-13 · Cited by 35
A novel bisphosphonate inhibitor of squalene synthase combined with a statin or a nitrogenous bisphosphonate in vitro
Wasko, B. M., Smits, J. P., Shull, L. W., Wiemer, D. F., & Hohl, R. J. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m016089 · Cited by 35
Development and validation of methodologies for the quantification of phytosterols and phytosterol oxidation products in cooked and baked food products
Menéndez-Carreño, M., Knol, D., & Janssen, H. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.09.073 · Cited by 36
Rapid microwave assisted extraction of meat lipids
Medina, A. L., da Silva, M. A. O., Barbosa, H. D. S., et al. (2015) · Food Research International · doi:10.1016/j.foodres.2015.10.028 · Cited by 35
The Granuloma Response Controlling Cryptococcosis in Mice Depends on the Sphingosine Kinase 1–Sphingosine 1-Phosphate Pathway
Farnoud, A. M., Bryan, A. M., Kechichian, T. B., Luberto, C., & Poeta, M. D. (2015) · Infection and Immunity · doi:10.1128/iai.00056-15 · Cited by 35
Transbilayer asymmetry and sphingomyelin composition modulate the preferential membrane partitioning of the nicotinic acetylcholine receptor in Lo domains
Perillo, V. L., Peñalva, D. A., Vitale, A. J., Barrantes, F. J., & Antollini, S. S. (2015) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2015.12.003 · Cited by 34
Effects of combined PPAR-γ and PPAR-α agonist therapy on fructose induced NASH in rats: Modulation of gene expression
El-Haleim, E. A. A., Bahgat, A. K., & Saleh, S. (2016) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2016.01.011 · Cited by 35
Biohydrogenation of Fatty Acids Is Dependent on Plant Species and Feeding Regimen of Dairy Cows
Petersen, M. B., & Jensen, S. K. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf405552m · Cited by 35
Utilization of soak liquor in microbial fuel cell
Selvaraj, R., Vidhya, S., Krishnaraj, R., et al. (2016) · Fuel · doi:10.1016/j.fuel.2016.04.121 · Cited by 35
Circulating ceramides are inversely associated with cardiorespiratory fitness in participants aged 54–96 years from the Baltimore Longitudinal Study of Aging
Fabbri, E., An, Y., Simonsick, E. M., Chia, C. W., Zoli, M., Haughey, N. J., Mielke, M. M., Ferrucci, L., & Coen, P. M. (2016) · Aging Cell · doi:10.1111/acel.12491 · Cited by 36
Weak aggregation: State of the art, expectations and open questions
Zemb, T. N., & Kunz, W. (2016) · Current Opinion in Colloid & Interface Science · doi:10.1016/j.cocis.2016.04.002 · Cited by 35
Lipid deregulation in UV irradiated skin cells: Role of 25-hydroxycholesterol in keratinocyte differentiation during photoaging
Olivier, E., Dutot, M., Regazzetti, A., Dargère, D., Auzeil, N., Laprévôte, O., & Rat, P. (2016) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2016.05.015 · Cited by 38
DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli
Si, H.-M., Fa, Z., Wu, A.-N., Han, R.-Z., Xu, G., & Ni, Y. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0527-9 · Cited by 35
Grassland to woodland transitions: Dynamic response of microbial community structure and carbon use patterns
Creamer, C. A., Filley, T. R., Boutton, T. W., & Rowe, H. I. (2016) · Journal of Geophysical Research Biogeosciences · doi:10.1002/2016jg003347 · Cited by 35
Trophic interactions among pelagic sharks and large predatory teleosts in the northeast central Pacific
Li, Y., Zhang, Y., & Dai, X. (2016) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2016.04.013 · Cited by 35
Seasonal Variations in Lipid Content, Fatty Acid Composition and Nutritional Profiles of Five Freshwater Fish from the Amazon Basin
Petenuci, M. E., Rocha, I. D. N. A., de Sousa, S. C., et al. (2016) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-016-2884-8 · Cited by 35
Influence of Lactobacillus buchneri as silage additive and forage:concentrate ratio on the growth performance, fatty acid profile in longissimus muscle, and meat quality of beef cattle
Rabêlo, C. H. S., Basso, F. C., McAllister, T. A., Lage, J. F., Gonçalves, G. S., Lara, E. C., Oliveira, A. A. M. D. A., Berchielli, T. T., & Reis, R. A. (2016) · Canadian Journal of Animal Science · doi:10.1139/cjas-2015-0161 · Cited by 44
Comprehensive optimization of LC–MS metabolomics methods using design of experiments (COLMeD)
Rhoades, S. D., & Weljie, A. M. (2016) · Metabolomics · doi:10.1007/s11306-016-1132-4 · Cited by 35
Chemical Composition and Functional Properties of Selected Seaweeds from the Kenya Coast
Muraguri, E. N., Wakibia, J., & Kinyuru, J. N. (2016) · Journal of Food Research · doi:10.5539/jfr.v5n6p114 · Cited by 35
Dietary intake of n-3 PUFAs modifies the absorption, distribution and bioavailability of fatty acids in the mouse gastrointestinal tract
Yang, Q., Wang, S., Ji, Y., Chen, H., Zhang, H., Chen, W., Gu, Z., & Chen, Y. Q. (2017) · Lipids in Health and Disease · doi:10.1186/s12944-016-0399-9 · Cited by 35
NMR-Based Lipid Profiling of High Density Lipoprotein Particles in Healthy Subjects with Low, Normal, and Elevated HDL-Cholesterol
Kostara, C. E., Tsimihodimos, V., Elisaf, M., & Bairaktari, E. T. (2017) · Journal of Proteome Research · doi:10.1021/acs.jproteome.6b00975 · Cited by 35
Isotopic niche partitioning between two apex predators over time
Drago, M., Cardona, L., Franco‐Trecu, V., et al. (2017) · Journal of Animal Ecology · doi:10.1111/1365-2656.12666 · Cited by 35
Characterization of a secretory hydrolase from Mycobacterium tuberculosis sheds critical insight into host lipid utilization by M. tuberculosis
Singh, K. H., Jha, B., Dwivedy, A., Choudhary, E., Arpitha, G. N., Ashraf, A., Arora, D., Agarwal, N., & Biswal, B. K. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.794297 · Cited by 35
Proximate composition and mineral contents in the body wall of two species of sea cucumber from Oman Sea
Barzkar, N., Fariman, G. A., & Taheri, A. (2017) · Environmental Science and Pollution Research · doi:10.1007/s11356-017-9379-5 · Cited by 35
Extraction and detailed characterization of phospholipid-enriched oils from six species of edible clams
Liu, Z., Zhou, D., Wu, Z., Yin, F., Zhao, Q., Xie, H., Zhang, J.-R., Qin, L., & Shahidi, F. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.07.035 · Cited by 34
Semicontinuous outdoor cultivation and efficient harvesting of marine Chlorella vulgaris BDUG 91771 with minimum solid co-precipitation and high floc recovery for biodiesel
Mathimani, T., Bhumathi, D., Ahamed, T. S., Dineshbabu, G., Garlapati, D., Uma, L., & Prabaharan, D. (2017) · Energy Conversion and Management · doi:10.1016/j.enconman.2017.06.077 · Cited by 35
Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer
Fukano, H., Wada, S., Kurata, O., et al. (2017) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.002028 · Cited by 35
Effects of marsh cultivation and restoration on soil microbial communities in the Sanjiang Plain, Northeastern China
Xu, S., Zhang, B., Ma, L., et al. (2017) · European Journal of Soil Biology · doi:10.1016/j.ejsobi.2017.08.010 · Cited by 35
Pork skin and canola oil as strategy to confer technological and nutritional advantages to burgers
Fagundes, D. T. D. O., Lorenzo, J. M., dos Santos, B. A., et al. (2017) · Czech Journal of Food Sciences · doi:10.17221/67/2017-cjfs · Cited by 35
Removals of estrone and 17β-estradiol by microalgae cultivation: kinetics and removal mechanisms
Ruksrithong, C., & Phattarapattamawong, S. (2017) · Environmental Technology · doi:10.1080/09593330.2017.1384068 · Cited by 36
Textural, Color, Hygroscopic, Lipid Oxidation, and Sensory Properties of Cookies Containing Free and Microencapsulated Chia Oil
de Almeida, M. M. C., Francisco, C. R. L., de Oliveira, A., et al. (2018) · Food and Bioprocess Technology · doi:10.1007/s11947-018-2057-x · Cited by 35
Mechanistic kinetic models describing impact of early attachment between Chlorella vulgaris and polyurethane foam material in fluidized bed bioreactor on lipid for biodiesel production
Mohd-Sahib, A.-A., Lim, J. W., Lam, M. K., Uemura, Y., Ho, C., Oh, W., & Tan, W. (2018) · Algal Research · doi:10.1016/j.algal.2018.05.017 · Cited by 34
Inhibition of glycosphingolipid synthesis reverses skin inflammation and hair loss in ApoE−/− mice fed western diet
Bedja, D., Yan, W., Lad, V., Iocco, D., Sivakumar, N., Bandaru, V. V. R., & Chatterjee, S. B. (2018) · Scientific Reports · doi:10.1038/s41598-018-28663-9 · Cited by 36
Bioaccumulation and Biotransformation of Triclosan and Galaxolide in the Freshwater Oligochaete Limnodrilus hoffmeisteri in a Water/Sediment Microcosm
Peng, F., Ying, G., Pan, C., et al. (2018) · Environmental Science & Technology · doi:10.1021/acs.est.8b02637 · Cited by 35
Copper and ocean acidification interact to lower maternal investment, but have little effect on adult physiology of the Sydney rock oyster Saccostrea glomerata
Scanes, E., Parker, L. M., O’Connor, W. A., et al. (2018) · Aquatic Toxicology · doi:10.1016/j.aquatox.2018.07.020 · Cited by 35
Structure and inflammatory activity of the LPS isolated from Acetobacter pasteurianus CIP103108
Pallach, M., Lorenzo, F. D., Facchini, F. A., et al. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.08.035 · Cited by 35
Soil microarthropods alter the outcome of plant-soil feedback experiments
Kuťáková, E., Cesarz, S., Münzbergová, Z., & Eisenhauer, N. (2018) · Scientific Reports · doi:10.1038/s41598-018-30340-w · Cited by 37
The Impact of Heat-moisture Treatment on Properties of Musa paradisiaca L. Starch, and Optimization of Process Variables
Cordeiro, M. J. M., Veloso, C. M., Santos, L. S., et al. (2018) · Food Technology and Biotechnology · doi:10.17113/ftb.56.04.18.5490 · Cited by 35
Biomass yield and biochemical profile of fourteen species of fast-growing green algae from eutrophic bloomed freshwaters of Kerala, South India
Santhakumaran, P., Kookal, S. K., & Ray, J. G. (2018) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2018.09.021 · Cited by 34
Modulation of Litter Decomposition by the Soil Microbial Food Web Under Influence of Land Use Change
Heijboer, A., de Ruiter, P. C., Bodelier, P. L. E., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02860 · Cited by 35
High Throughput Screening Technologies in Biomass Characterization
Decker, S. R., Harman-Ware, A. E., Happs, R. M., Wolfrum, E. J., Tuskan, G. A., Kainer, D., Oguntimein, G. B., Rodríguez, M., Weighill, D. A., Jones, P. C., et al. (2018) · Frontiers in Energy Research · doi:10.3389/fenrg.2018.00120 · Cited by 35
A T-cell receptor escape channel allows broad T-cell response to CD1b and membrane phospholipids
Shahine, A., Reinink, P., Reijneveld, J. F., Gras, S., Holzheimer, M., Cheng, T., Minnaard, A. J., Altman, J. D., Lenz, S., Prandi, J., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07898-0 · Cited by 37
Regulation of Dietary Lipid Sources on Tissue Lipid Classes and Mitochondrial Energy Metabolism of Juvenile Swimming Crab, Portunus trituberculatus
Yuan, Y., Sun, P., Jin, M., Wang, X., & Zhou, Q. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00454 · Cited by 35
Low-fat Brazilian cooked sausage-Paio – with added oat fiber and inulin as a fat substitute: effect on the technological properties and sensory acceptance
SOUZA, C. V. B., Bellucci, E. R. B., Lorenzo, J. M., et al. (2019) · Food Science and Technology · doi:10.1590/fst.03618 · Cited by 35
Strategy of adjusting recirculation ratio for biohythane production via recirculated temperature-phased anaerobic digestion of food waste
Qin, Y., Wu, J., Xiao, B., Cong, M., Hojo, T., Cheng, J., & Li, Y.-Y. (2019) · Energy · doi:10.1016/j.energy.2019.04.182 · Cited by 33
The impact of wildlife tourism on the foraging ecology and nutritional condition of an apex predator
Meyer, L., Pethybridge, H., Beckmann, C. L., et al. (2019) · Tourism Management · doi:10.1016/j.tourman.2019.04.025 · Cited by 35
Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica
Bhatnagar, V. S., Bandyopadhyay, P., Rajacharya, G. H., Sarkar, S., Poluri, K. M., & Kumar, S. B. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1516-6 · Cited by 35
Benefits of the inclusion of açai oil in the diet of dairy sheep in heat stress on health and milk production and quality
dos Santos, D. D. S., Klauck, V., Campigotto, G., et al. (2019) · Journal of Thermal Biology · doi:10.1016/j.jtherbio.2019.07.007 · Cited by 35
Oleic acid-containing semisolid dosage forms exhibit in vivo anti-inflammatory effect via glucocorticoid receptor in a UVB radiation-induced skin inflammation model
Pegoraro, N. S., Camponogara, C., Gehrcke, M., Giuliani, L. M., da Silva, D. T., Maurer, L. H., Dias, P., Emanuelli, T., Cruz, L., & Oliveira, S. M. (2019) · Inflammopharmacology · doi:10.1007/s10787-019-00675-5 · Cited by 36
Anticancer Effect of Metformin in Herceptin-Conjugated Liposome for Breast Cancer
Lee, J., Shin, D. H., & Kim, J. (2019) · Pharmaceutics · doi:10.3390/pharmaceutics12010011 · Cited by 37
Biological nutrient recovery from human urine by enriching mixed microalgal consortium for biodiesel production
Behera, B., Patra, S., & Balasubramanian, P. (2020) · Journal of Environmental Management · doi:10.1016/j.jenvman.2020.110111 · Cited by 35
Compounds that modulate AMPK activity and hepatic steatosis impact the biosynthesis of microRNAs required to maintain lipid homeostasis in hepatocytes
Latorre, J., Ortega, F. J., Liñares‐Pose, L., Moreno‐Navarrete, J. M., Lluch, A., Comas, F., Oliveras‐Cañellas, N., Ricart, W., Höring, M., Zhou, Y., et al. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.102697 · Cited by 35
Using benchtop NMR spectroscopy as an online non-invasive in vivo lipid sensor for microalgae cultivated in photobioreactors
Bouillaud, D., Drouin, D., Charrier, B., Jacquemmoz, C., Farjon, J., Giraudeau, P., & Gonçalves, O. (2020) · Process Biochemistry · doi:10.1016/j.procbio.2020.03.016 · Cited by 35
Olanzapine-induced liver injury in mice: aggravation by high-fat diet and protection with sulforaphane
Isaacson, R. H., Beier, J. I., Khoo, N. K. H., Freeman, B. Α., Freyberg, Z., & Arteel, G. E. (2020) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2020.108399 · Cited by 34
HILIC-ESI-FTMS with All Ion Fragmentation (AIF) Scans as a Tool for Fast Lipidome Investigations
Ventura, G., Bianco, M., Calvano, C. D., Losito, I., & Cataldi, T. R. I. (2020) · Molecules · doi:10.3390/molecules25102310 · Cited by 36
“The Bligh and Dyer protocol [42] was used to obtain lipids from the investigated samples.”
Method
Chemical profile of Eugenia brasiliensis (Grumixama) pulp by PS/MS paper spray and SPME-GC / MS solid-phase microextraction
Ramos, A. L. C. C., Mendes, D. D., Silva, M. R., et al. (2020) · Research Society and Development · doi:10.33448/rsd-v9i7.4008 · Cited by 35
Flavobacterium pokkalii sp. nov., a novel plant growth promoting native rhizobacteria isolated from pokkali rice grown in coastal saline affected agricultural regions of southern India, Kerala
Menon, R. R., Kumari, S., Viver, T., & Rameshkumar, N. (2020) · Microbiological Research · doi:10.1016/j.micres.2020.126533 · Cited by 35
Evidence of Vent-Adaptation in Sponges Living at the Periphery of Hydrothermal Vent Environments: Ecological and Evolutionary Implications
Georgieva, M. N., Taboada, S., Riesgo, A., Díez‐Vives, C., Léo, F. C. D., Jeffreys, R. M., Copley, J., Little, C. T. S., Ríos, P., Cristobo, J., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01636 · Cited by 35
“Prior to stable carbon isotope analyses, lipids were extracted from sponges using a modified method of Bligh and Dyer (1959).”
Method
Optimization of pH, time, temperature, variety and concentration of the added fatty acid and the initial count of added lactic acid Bacteria strains to improve microbial conjugated linoleic acid production in fermented ground beef
Özer, C. O., & Kılıç, B. (2020) · Meat Science · doi:10.1016/j.meatsci.2020.108303 · Cited by 35
In-vitro anti-inflammatory activity, free radical (DPPH) scavenging, and ferric reducing ability (FRAP) of Sargassum cristaefolium lipid-soluble fraction and putative identification of bioactive compounds using UHPLC-ESI-ORBITRAP-MS/MS
Saraswati, S., Giriwono, P. E., Iskandriati, D., et al. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109702 · Cited by 35
Utilization of wastewater as nutrient media and biomass valorization in marine Chrysophytes- Chaetoceros and Isochrysis
Singh, P. K., Bhattacharjya, R., Saxena, A., Mishra, B., & Tiwari, A. (2020) · Energy Conversion and Management X · doi:10.1016/j.ecmx.2020.100062 · Cited by 35
Characteristics of Different Size Phytoplankton for Primary Production and Biochemical Compositions in the Western East/Japan Sea
Kang, J. J., Jang, H. K., Lim, J., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.560102 · Cited by 35
“Each biochemical compound (CHO, PRT, and LIP) was analyzed at the laboratory based on the methods of Lowry et al. (1951); Dubois et al. (1956), and Bligh and Dyer (1959), respectively.”
Method
The dysregulation of metabolic pathways and induction of the pentose phosphate pathway in renal ischaemia–reperfusion injury
Scantlebery, A. M. L., Tammaro, A., Mills, J. D., et al. (2020) · The Journal of Pathology · doi:10.1002/path.5605 · Cited by 35
“Lipids were extracted from homogenised tissues by Bligh and Dyer’s chloroform/methanol extraction method [15].”
Method
1H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth
Gnocchi, D., Coco, L. D., Girelli, C. R., Castellaneta, F., Cesari, G., Sabbà, C., Fanizzi, F. P., & Mazzocca, A. (2021) · Scientific Reports · doi:10.1038/s41598-020-78867-1 · Cited by 34
Changes in the Plant β-Sitosterol/Stigmasterol Ratio Caused by the Plant Parasitic Nematode Meloidogyne incognita
Cabianca, A., Müller, L., Pawlowski, K., & Dahlin, P. (2021) · Plants · doi:10.3390/plants10020292 · Cited by 37
“Sterols were extracted according to Bligh and Dyer [51].”
Method
Glycolate combats massive oxidative stress by restoring redox potential in Caenorhabditis elegans
Diez, V., Traikov, S., Schmeißer, K., Adhikari, A. K. D., & Kurzchalia, T. V. (2021) · Communications Biology · doi:10.1038/s42003-021-01669-2 · Cited by 34
Sodium chloride reduction in fresh sausages using salt encapsulated in carnauba wax
Beck, P. H. B., Matiucci, M. A., Neto, A. Á. M., et al. (2021) · Meat Science · doi:10.1016/j.meatsci.2021.108462 · Cited by 35

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks