Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 23,501–23,600 of 48,846

Differential Influence of Soluble Dietary Fibres on Intestinal and Hepatic Carbohydrate Response
Pontifex, M. G., Mushtaq, A., Gall, G. L., Rodríguez‐Ramiro, I., Blokker, B., Hoogteijling, M. E. M., Ricci, M. R., Pellizzon, M., Vauzour, D., & Müller, M. M. (2021) · Nutrients · doi:10.3390/nu13124278 · Cited by 25
Exogenous fatty acids affect membrane properties and cold adaptation of Listeria monocytogenes
Flegler, A., Iswara, J., Mänz, A. T., Schocke, F. S., Faßbender, W. A., Hölzl, G., & Lipski, A. (2022) · Scientific Reports · doi:10.1038/s41598-022-05548-6 · Cited by 24
Discovery of coordinately regulated pathways that provide innate protection against interbacterial antagonism
Ting, S., LaCourse, K. D., Ledvina, H. E., et al. (2022) · eLife · doi:10.7554/elife.74658 · Cited by 24
“Cells were then collected, and the lipids were extracted using the Bligh–Dyer method ( Bligh and Dyer, 1959 ).”
Prediction of Neonatal Respiratory Distress Biomarker Concentration by Application of Machine Learning to Mid-Infrared Spectra
Ahmed, W., Veluthandath, A. V., Rowe, D. J., Madsen, J., Clark, H. W., Postle, A. D., Wilkinson, J. S., & Murugan, G. S. (2022) · Sensors · doi:10.3390/s22051744 · Cited by 25
A cellular and molecular atlas reveals the basis of chytrid development
Laundon, D., Chrismas, N. A. M., Bird, K. E., Thomas, S. R., Möck, T., & Cunliffe, M. (2022) · eLife · doi:10.7554/elife.73933 · Cited by 27
Neutral sphingomyelinase 2 inhibition attenuates extracellular vesicle release and improves neurobehavioral deficits in murine HIV
Zhu, X., Hollinger, K. R., Huang, Y., Borjabad, A., Kim, B., Arab, T., Thomas, A. G., Moniruzzaman, M., Lovell, L. E., Turchinovich, A. A., et al. (2022) · Neurobiology of Disease · doi:10.1016/j.nbd.2022.105734 · Cited by 28
Carbon-induced effective lipid accumulation and self-flocculation for biofuel production of Tetradesmus obliquus FACHB-12
Mi, Y., Yang, S., Chen, J., Li, Z., Dong, L., Qun, W., Tang, Y., & Ma, X. (2022) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2022.131813 · Cited by 24
Sprouting and Hydrolysis as Biotechnological Tools for Development of Nutraceutical Ingredients from Oat Grain and Hull
Jiménez-Pulido, I. J., Rico, D., Martínez‐Villaluenga, C., Pérez‐Jiménez, J., de Luis, D. A., & Martín‐Diana, A. B. (2022) · Foods · doi:10.3390/foods11182769 · Cited by 23
“Lipids were extracted according to the method of Bligh and Dyer [32].”
Method
Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model
Tyrtyshnaia, A. A., Konovalova, S. P., Ponomarenko, A. I., Egoraeva, A. A., & Manzhulo, I. (2022) · Nutrients · doi:10.3390/nu14183879 · Cited by 25
“After the addition of 0.1 nmol internal standard 22:0-NAE to the frozen mouse brain, lipid extraction was performed according to the method of Bligh and Dyer (1959) [37] Ice-cold CHCl3 (1 mL) was used to restore the final lipid residue.”
MethodInfluential
Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
Schiaffarino, O., González, D. V., García-Pérez, I. M., et al. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2022.910936 · Cited by 24
“Lipid extraction of the isolated OMM and IMMmembranes was performed according to Bligh and Dyer (Bligh and Dyer, 1959), where 0.”
MethodInfluential
Hemp seed significantly modulates the endocannabinoidome and produces beneficial metabolic effects with improved intestinal barrier function and decreased inflammation in mice under a high-fat, high-sucrose diet as compared with linseed
Necib, R. B., Manca, C., Lacroix, S., Martin, C., Flamand, N., Marzo, V. D., & Silvestri, C. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.882455 · Cited by 24
“Lipids were extracted from tissue samples according to the Bligh and Dyer method (46) with slight modifications.”
Method
Retinoic Acid Receptor Alpha (RARα) in Macrophages Protects from Diet-Induced Atherosclerosis in Mice
Bawa, F. N. C., Gopoju, R., Xu, Y., Hu, S., Zhu, Y., Chen, S., Jadhav, K. S., & Zhang, Y. (2022) · Cells · doi:10.3390/cells11203186 · Cited by 23
“Mouse primary peritoneal macrophages or 100 mg liver were homogenized in methanol, and lipids were extracted in chloroform/methanol (2:1 v/v) as described previously [23].”
High Concentrate Flavonoids Extract from Citrus Pomace Using Enzymatic and Deep Eutectic Solvents Extraction
de Oliveira, J. A. R., Barbosa, P. D. P. M., & Macêdo, G. A. (2022) · Foods · doi:10.3390/foods11203205 · Cited by 25
Chemical composition and prebiotic activity of baru (Dipteryx alata Vog.) pulp on probiotic strains and human colonic microbiota
Alves, A. M., Sampaio, K. B., Lima, M. D. S., et al. (2022) · Food Research International · doi:10.1016/j.foodres.2022.112366 · Cited by 24
Effects of Yellow Mealworm (Tenebrio molitor) on Growth Performance, Hepatic Health and Digestibility in Juvenile Largemouth Bass (Micropterus salmoides)
Chen, H., Yu, J., Ran, X., et al. (2023) · Animals · doi:10.3390/ani13081389 · Cited by 24
“Total lipid in liver was measured following the method of Bligh and Dyer [20] and all chemical composition analysis of diets and whole body were conducted by Association of Official Analytical Chemists standard methods [21].”
Method
Enhancement of safety and quality of ready-to-cook Asian green mussel using acidic electrolyzed water depuration in combination with sous vide cooking
Palamae, S., Temdee, W., Buatong, J., et al. (2023) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2023.103391 · Cited by 24
Ceramide generation during curcumin-induced apoptosis is controlled by crosstalk among Bcl-2, Bcl-xL, caspases and glutathione
Shakor, A. B. A., Atia, M. M., Alshehri, A. S., Sobota, A., & Kwiatkowska, K. (2015) · Cellular Signalling · doi:10.1016/j.cellsig.2015.07.022 · Cited by 24
Lipid transfer particle-induced transformation of human high density lipoprotein into apolipoprotein A-I-deficient low density particles.
Silver, E. T., Scraba, D. G., & Ryan, R. O. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)45731-6 · Cited by 24
Administration of RRR-α-tocopherol to pregnant mares stimulates maternal IgG and IgM production in colostrum and enhances vitamin E and IgM status in foals
Bondo, T., & Jensen, S. K. (2010) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/j.1439-0396.2010.01043.x · Cited by 24
Cross-Generational trans Fat Consumption Favors Self-Administration of Amphetamine and Changes Molecular Expressions of BDNF, DAT, and D1/D2 Receptors in the Cortex and Hippocampus of Rats
Kuhn, F. T., Dias, V. T., Roversi, K., Vey, L. T., de Freitas, D. L., Pase, C. S., Roversi, K., Veit, J. C., Emanuelli, T., & Bürger, M. E. (2015) · Neurotoxicity Research · doi:10.1007/s12640-015-9549-5 · Cited by 26
Effect of crossbreeding with L imousine, R ubia G allega and B elgium B lue on meat quality and fatty acid profile of H olstein calves
Domingo, G., Iglesias, A., Monserrat, L., et al. (2015) · Animal Science Journal · doi:10.1111/asj.12373 · Cited by 24
Dietary fenugreek and onion attenuate cholesterol gallstone formation in lithogenic diet–fed mice
Reddy, R. R., & Srinivasan, K. (2011) · International Journal of Experimental Pathology · doi:10.1111/j.1365-2613.2011.00782.x · Cited by 24
LIPID, CHOLESTEROL AND FATTY ACID PROFILE OF SOME COMMERCIALLY IMPORTANT FISH SPECIES FROM SOUTH CASPIAN SEA
Pirestani, S., Sahari, M. A., Barzegar, M., & Nikoopour, H. (2010) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2010.00343.x · Cited by 24
Different strategies of energy storage in cultured and freshly isolated Symbiodinium sp.
Wang, L.-H., Chen, H., Jhu, C., et al. (2015) · Journal of Phycology · doi:10.1111/jpy.12349 · Cited by 24
Sphingopyxis flava sp. nov., isolated from a hexachlorocyclohexane (HCH)-contaminated soil
Verma, H., Rani, P., Singh, A. K., et al. (2015) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.000482 · Cited by 24
Haemolymph Protein and Lipid Profile of Rhipicephalus (Boophilus) microplus Infected by Fungi
Ângelo, I. D. C., Gôlo, P. S., Camargo, M. G., et al. (2010) · Transboundary and Emerging Diseases · doi:10.1111/j.1865-1682.2010.01119.x · Cited by 24
Diacylglycerol kinase‐dependent formation of phosphatidic acid molecular species during interleukin‐2 activation in CTLL‐2 T‐lymphocytes
Mizuno, S., Sakai, H., Saito, M., et al. (2012) · FEBS Open Bio · doi:10.1016/j.fob.2012.08.006 · Cited by 24
Fatty acids as tracers of trophic interactions between seston, mussels and biodeposits in a coastal embayment of mussel rafts in the proximity of fish cages
Irisarri, J., Fernández-Reiriz, M., Troch, M. D., & Labarta, U. (2014) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/j.cbpb.2014.04.006 · Cited by 24
Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
Kennedy, J., Margassery, L. M., O’Leary, N. D., et al. (2013) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.049650-0 · Cited by 24
Comparative Effects of Sandalwood Seed Oil on Fatty Acid Profiles and Inflammatory Factors in Rats
Li, G., Singh, A., Liu, Y., et al. (2012) · Lipids · doi:10.1007/s11745-012-3752-4 · Cited by 24
Soil water availability and microsite mediate fungal and bacterial phospholipid fatty acid biomarker abundances in Mojave Desert soils exposed to elevated atmospheric CO2
Jin, V., Schaeffer, S., Ziegler, S., et al. (2011) · Journal of Geophysical Research Atmospheres · doi:10.1029/2010jg001564 · Cited by 27
Alterations of microbial populations and composition in the rhizosphere and bulk soil as affected by residual acetochlor
Bai, Z., Xu, H., He, H., et al. (2012) · Environmental Science and Pollution Research · doi:10.1007/s11356-012-1061-3 · Cited by 24
OlsG (Sinac_1600) Is an Ornithine Lipid N-Methyltransferase from the Planctomycete Singulisphaera acidiphila
Escobedo‐Hinojosa, W., Vences‐Guzmán, M. Á., Schubotz, F., et al. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.639575 · Cited by 24
Identification of a phospholipase B encoded by the LPL1 gene in Saccharomyces cerevisiae
Kandasamy, S., Rajakumar, S., & Nachiappan, V. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2014.06.013 · Cited by 24
Characterization and Quantification of the Aliphatic Hydrocarbon Fraction during Linseed Development (Linum usitatissimum L.)
Herchi, W., Harrabi, S., Rochut, S., et al. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9004884 · Cited by 24
Phospholipid fatty acids of a marine sedimentary microbial community in a laboratory microcosm: Responses to petroleum hydrocarbon contamination
Syakti, A. D., Mazzella, N., Nérini, D., et al. (2006) · Organic Geochemistry · doi:10.1016/j.orggeochem.2006.01.009 · Cited by 24
Ultrasonic and sensory characterization of dry-cured ham fat from Iberian pigs with different genetics and feeding backgrounds
Niñoles, L., Sanjuán, N., Ventanas, S., et al. (2008) · Meat Science · doi:10.1016/j.meatsci.2008.04.008 · Cited by 24
Unravelling the matrix effect of fresh sampled cells for in vivo unbiased FTIR determination of the absolute concentration of total lipid content of microalgae
Coat, R., Montalescot, V., León, E. S., Kucma, D., Perrier, C., Jubeau, S., Thouand, G., Legrand, J., Pruvost, J., & Gonçalves, O. (2014) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-014-1194-5 · Cited by 24
Lipid Analysis ofCoffea ArabicaLinn. Beans and Their Possible Hypercholesterolemic Effects
Al-Kanhal, M. A. (1997) · International Journal of Food Sciences and Nutrition · doi:10.3109/09637489709006973 · Cited by 24
The Lipid Composition Modulates the Influence of the Bovine Seminal Plasma Protein PDC-109 on Membrane Stability
Tannert, A., Töpfer‐Petersen, E., Herrmann, A., et al. (2007) · Biochemistry · doi:10.1021/bi7011299 · Cited by 24
ApoA-I induces a preferential efflux of monounsaturated phosphatidylcholine and medium chain sphingomyelin species from a cellular pool distinct from HDL3 mediated phospholipid efflux
Schifferer, R., Liebisch, G., Bandulik, S., et al. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2007.04.011 · Cited by 24
The Role of Phospholipid Headgroup Composition and Trehalose in the Desiccation Tolerance of Caenorhabditis elegans
Abusharkh, S. E., Erkut, C., Oertel, J., et al. (2014) · Langmuir · doi:10.1021/la502654j · Cited by 24
Perinatal programming of murine immune responses by polyunsaturated fatty acids
van Vlies, N., Hogenkamp, A., Fear, A. L., van Esch, B. C. A. M., Oosting, A., van de Heijning, B., van der Beek, E. M., Calder, P. C., & Garssen, J. (2010) · Journal of Developmental Origins of Health and Disease · doi:10.1017/s204017441000067x · Cited by 24
A study of lipid- and water-soluble arsenic species in liver of Northeast Arctic cod (Gadus morhua) containing high levels of total arsenic
Sele, V., Sloth, J. J., Julshamn, K., Skov, K., & Amlund, H. (2015) · Journal of Trace Elements in Medicine and Biology · doi:10.1016/j.jtemb.2014.12.010 · Cited by 25
Increasing algal biofuel production using Nannocholropsis oculata cultivated with anaerobically and aerobically treated swine wastewater
Wu, P., Teng, J.-C., Lin, Y., & Hwang, S. J. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.109 · Cited by 24
Membrane Structure Correlates to Function of LLP2 on the Cytoplasmic Tail of HIV-1 gp41 Protein
Boscia, A. L., Akabori, K., Benamram, Z., Michel, J., Jablin, M. S., Steckbeck, J. D., Montelaro, R. C., Nagle, J. F., & Tristram‐Nagle, S. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2013.06.042 · Cited by 24
Effects of dietary fat saturation on fatty acid composition and gene transcription in different tissues of Iberian pigs
Benítez, R., Núñez, Y., Fernández, A. I., Isabel, B., Fernández, A. I., Rodríguez, C., Barragán, C., Palomino, P. M., López-Bote, C. J., Silio, L. F., et al. (2014) · Meat Science · doi:10.1016/j.meatsci.2014.12.005 · Cited by 24
Corn silage with and without enzyme-bacteria inoculants on performance, carcass characteristics and meat quality in feedlot finished crossbred bulls
Fugita, C. A., do Prado, I. N., Jobim, C. C., et al. (2012) · Revista Brasileira de Zootecnia · doi:10.1590/s1516-35982012000100023 · Cited by 24
Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity
Xie, X., Liao, M., Ma, A., et al. (2011) · Acta Geochimica · doi:10.1007/s11631-011-0521-7 · Cited by 24
Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry
Alberici, L. C., Oliveira, H. C. F., Catharino, R. R., Vercesi, A. E., Eberlin, M. N., & Alberici, R. M. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-5208-8 · Cited by 24
Effect of exercise training on liver antioxidant enzymes in STZ-diabetic rats
de Lima, T. I., Monteiro, I. C. C. D. R., Valença, S. S., Leal‐Cardoso, J. H., Fortunato, R. S., de Carvalho, D. P., Teodoro, B. G., & Ceccatto, V. M. (2015) · Life Sciences · doi:10.1016/j.lfs.2015.01.031 · Cited by 24
Effect of Dietary Lactate on Growth and Chemical Composition of Arctic charr Salvelinus alpinus
Ringø, E., Olsen, R. E., & Castell, J. D. (1994) · Journal of the World Aquaculture Society · doi:10.1111/j.1749-7345.1994.tb00234.x · Cited by 24
Dinapinones, novel inhibitors of triacylglycerol synthesis in mammalian cells, produced by Penicillium pinophilum FKI-3864
Ohte, S., Matsuda, D., Uchida, R., Nonaka, K., Masuma, R., Ōmura, S., & Tomoda, H. (2011) · The Journal of Antibiotics · doi:10.1038/ja.2011.32 · Cited by 24
Behavioural, physiological and biochemical markers in damselfly larvae (Ischnura elegans) to assess effects of accumulated metal mixtures
Praet, N. V., Jonge, M. D., Blust, R., et al. (2013) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2013.09.093 · Cited by 24
Comparison of methods for extraction of organic N monomers from soil microbial biomass
Warren, C. R. (2014) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2014.11.005 · Cited by 24
Lipid classes and fatty acid compositions of wild and cultured sweet smelt Plecoglossus altivelis muscles and eggs in Korea+
Jeong, B., Moon, S.-K., Jeong, W.-G., et al. (2000) · Fisheries Science · doi:10.1046/j.1444-2906.2000.00117.x · Cited by 24
Effects of Alcohol Compounds on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans
Huang, C., Wu, H., Liu, L., Lou, W., & Zong, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0046975 · Cited by 24
Enterobacillus tribolii gen. nov., sp. nov., a novel member of the family Enterobacteriaceae, isolated from the gut of a red flour beetle, Tribolium castaneum
Patil, V. S., Salunkhe, R. C., Patil, R. H., et al. (2015) · Antonie van Leeuwenhoek · doi:10.1007/s10482-015-0412-8 · Cited by 24
Highly specific methyl-end fatty-acid desaturases of trypanosomatids
Alloatti, A., & Uttaro, A. D. (2010) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2010.10.006 · Cited by 24
Hydrophilic interaction chromatography based solid-phase extraction and MALDI TOF mass spectrometry for revealing the influence of Pseudomonas fluorescens on phospholipids in salmon fillet
Shen, Q., Yang, Q. P., & Cheung, H. (2014) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-014-8365-8 · Cited by 24
SOIL GRINDING INCREASES THE RELATIVE ABUNDANCE OF EUKARYOTIC PHOSPHOLIPID FATTY ACIDS
Allison, V. J., & Miller, R. M. (2005) · Soil Science Society of America Journal · doi:10.2136/sssaj2005.0423 · Cited by 24
Severe hypertriglyceridemia and hypercholesterolemia accelerating renal injury: a novel model of type 1 diabetic hamsters induced by short-term high-fat / high-cholesterol diet and low-dose streptozotocin
He, L., Hao, L., Fu, X., Huang, M., & Li, R. (2015) · BMC Nephrology · doi:10.1186/s12882-015-0041-5 · Cited by 27
Effects of the dietary energy source on meat quality and eating quality attributes and fatty acid profile of Simmental bulls
Sami, A., Koegel, J., Eichinger, H. M., et al. (2006) · Animal Research · doi:10.1051/animres:2006016 · Cited by 24
Comparative study of tributyltin toxicity on two bacteria of the genus Bacillus
Martins, J. D., Jurado, A. S., Moreno, A. J., et al. (2005) · Toxicology in Vitro · doi:10.1016/j.tiv.2005.06.019 · Cited by 24
Anti methicillin-resistant Staphylococcus aureus (MRSA) activity by linolenic acid isolated from the marine microalga Chlorococcum HS-101
Ohta, S., Chang, T., Kawashima, A., Nagate, T., Murase, M., Nakanishi, H., Miyata, H., & Kondo, M. (1994) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/bf00195486 · Cited by 24
Integration of sewage sludge digestion with advanced biofuel synthesis
Liu, Z., Ruan, Z., Xiao, Y., et al. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.017 · Cited by 24
Inter species changes of lipid compositions in liver of shallow-water sharks from the Indian Ocean
Jayasinghe, C. V. L., Gotoh, N., Tokairin, S., et al. (2003) · Fisheries Science · doi:10.1046/j.1444-2906.2003.00668.x · Cited by 24
Extraction and characterization of lipids from Sarcocornia ambigua meal: a halophyte biomass produced with shrimp farm effluent irrigation
Costa, C. S. B., Vicenti, J. R. D. M., Morón-Villarreyes, J. A., et al. (2014) · Anais da Academia Brasileira de Ciências · doi:10.1590/0001-3765201420130022 · Cited by 24
Effect of Different Hydrolysates of Whey Protein on Hepatic Glutathione Content in Mice
Pacheco, M. T. B., & Sgarbieri, V. C. (2005) · Journal of Medicinal Food · doi:10.1089/jmf.2005.8.337 · Cited by 23
POTENTIAL OF SEAFOOD INDUSTRY BYPRODUCTS AS SOURCES OF RECOVERABLE LIPIDS: FATTY ACID COMPOSITION OF MEAT AND NONMEAT COMPONENT OF SELECTED INDIAN MARINE FISHES
RAI, A. K., Swapna, H. C., Bhaskar, N., & Baskaran, V. (2011) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2011.00549.x · Cited by 24
Nutritional value of Cymodocea nodosa and Posidonia oceanica along the western Egyptian Mediterranean coast
Din, N. G. S. E., & El-Sherif, Z. M. (2013) · The Egyptian Journal of Aquatic Research · doi:10.1016/j.ejar.2013.10.001 · Cited by 24
Halogenated persistent organic pollutants in relation to trophic level in deep sea fish
Webster, L., Russell, M., Walsham, P., et al. (2014) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2014.09.034 · Cited by 24
PAF-acetylhydrolase expressed during megakaryocyte differentiation inactivates PAF-like lipids
Foulks, J. M., Marathe, G. K., Michetti, N., Stafforini, D. M., Zimmerman, G. A., McIntyre, T. M., & Weyrich, A. S. (2009) · Blood · doi:10.1182/blood-2008-11-186312 · Cited by 24
Insertion of cyanobacterial desA gene coding for Δ12-acyl-lipid desaturase increases potato plant resistance to oxidative stress induced by hypothermia
Demin, I. N., Deryabin, A. N., Синькевич, М. С., et al. (2008) · Russian Journal of Plant Physiology · doi:10.1134/s1021443708050075 · Cited by 24
Impact of Organic Loads and Environmental Gradients on Microphytobenthos and Meiofaunal Distribution in a Coastal Lagoon
Gambi, C., Totti, C., & Manini, E. (2003) · Chemistry and Ecology · doi:10.1080/0275754031000119951 · Cited by 24
Recent Advances in Shotgun Lipidomics and Their Implication for Vision Research and Ophthalmology
Bhattacharya, S. K. (2013) · Current Eye Research · doi:10.3109/02713683.2012.760742 · Cited by 24
Seasonal variability in stable isotopes of estuarine consumers under different freshwater flow regimes
Olin, J. A., Hussey, N., Rush, S. A., et al. (2013) · Marine Ecology Progress Series · doi:10.3354/meps10402 · Cited by 24
Inhibition of Ubiquitin Ligase F-box and WD Repeat Domain-containing 7α (Fbw7α) Causes Hepatosteatosis through Krüppel-like Factor 5 (KLF5)/Peroxisome Proliferator-activated Receptor γ2 (PPARγ2) Pathway but Not SREBP-1c Protein in Mice
Kumadaki, S., Karasawa, T., Matsuzaka, T., Ema, M., Nakagawa, Y., Nakakuki, M., Saito, R., Yahagi, N., IWASAKI, H. O., Sone, H., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.235283 · Cited by 24
NMR-metabolic methodology in the study of GM foods.
Sobolev, A. P., Capitani, D., Giannino, D., et al. (2010) · PubMed · doi:10.3390/nu20100001 · Cited by 24
Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC
Witt, E. M. H. J., Davies, N. W., & Galinski, E. A. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3211-9 · Cited by 24
Sesamin modulation of lipid class and fatty acid profile in early juvenile teleost, Lates calcarifer, fed different dietary oils
Alhazzaa, R., Bridle, A. R., Carter, C. G., et al. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.04.004 · Cited by 24
The effects of barrier disruption and moisturization on the dynamic drying mechanics of human stratum corneum
Liu, X., & German, G. K. (2015) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2015.04.017 · Cited by 24
The effect of seaweed extracts, as a supplement or alternative culture medium, on the growth rate and biochemical composition of the microalga, Isochrysis galbana (Park 1949)
Rohani-Ghadikolaei, K., Ng, W., Abdulalian, E., Naser, A., & Yusuf, A. (2011) · Aquaculture Research · doi:10.1111/j.1365-2109.2011.02951.x · Cited by 24
Streptomyces barkulensis sp. nov., isolated from an estuarine lake
Ray, L. B., Mishra, S. R., Panda, A. N., et al. (2014) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.056614-0 · Cited by 24
Mdr P-glycoproteins are not essential for biliary excretion of the hydrophobic heme precursor protoporphyrin in a griseofulvin-induced mouse model of erythropoietic protoporphyria
Plösch, T., Bloks, V. W., Baller, J. F. W., et al. (2002) · Hepatology · doi:10.1053/jhep.2002.30900 · Cited by 24
Inter‐Tissue Differences in Fatty Acid Incorporation as a Result of Dietary Oil Manipulation in Port Jackson Sharks (Heterodontus portusjacksoni)
Beckmann, C. L., Mitchell, J. G., Stone, D. A. J., & Huveneers, C. P. M. (2014) · Lipids · doi:10.1007/s11745-014-3887-6 · Cited by 24
Comparative larval energetics of an ophiuroid and an echinoid echinoderm
Whitehill, E. A. G., & Moran, A. L. (2012) · Invertebrate Biology · doi:10.1111/j.1744-7410.2012.00277.x · Cited by 24
Characterization of Five Typical Agave Plants Used To Produce Mezcal through Their Simple Lipid Composition Analysis by Gas Chromatography
Martínez‐Aguilar, J., & Peña‐Álvarez, A. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf802141d · Cited by 24
Riboflavin requirements in on-growing rainbow trout, Oncorhynchus mykiss
Amezaga, M. R., & Knox, D. (1990) · Aquaculture · doi:10.1016/0044-8486(90)90322-e · Cited by 24
Intestinal lipid alterations occur prior to antibody-induced prostaglandin E2 production in a mouse model of ischemia/reperfusion
Sparkes, B. L., Slone, E. A., Roth, M. R., et al. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.01.004 · Cited by 24
Screening of Industrial Wastewaters as Feedstock for the Microbial Production of Oils for Biodiesel Production and High-Quality Pigments
Schneider, T., Graeff‐Hönninger, S., French, W. T., Hernandez, R. A., Claupein, W., Holmes, W. E., & Merkt, N. (2012) · Journal of Combustion · doi:10.1155/2012/153410 · Cited by 24
Effects of similar intakes of marinen-3 fatty acids from enriched food products and fish oil on cardiovascular risk markers in healthy human subjects
Kirkhus, B., Lamglait, A., Eilertsen, K., Falch, E., Haider, T., Vik, H., Hoem, N., Hagve, T., Basu, S., Olsen, E. F., et al. (2011) · British Journal Of Nutrition · doi:10.1017/s0007114511004508 · Cited by 25
Cholesteryl ester hydrolysis in J774 macrophages occurs in the cytoplasm and lysosomes
Avart, S., Bernard, D. W., Jerome, W. G., & Glick, J. M. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32444-5 · Cited by 24
Trans-10,cis-12-conjugated linoleic acid reduces the hepatic triacylglycerol content and the leptin mRNA level in adipose tissue in obese Zucker fa/fa rats
Gudbrandsen, O. A., Rodríguez, E., Wergedahl, H., Mørk, S. J., Reseland, J. E., Skorve, J., Palou, A., & Berge, R. K. (2009) · British Journal Of Nutrition · doi:10.1017/s0007114509297200 · Cited by 24
A Monogalactosyldiacylglycerol Synthase Found in the Green Sulfur Bacterium Chlorobaculum tepidum Reveals Important Roles for Galactolipids in Photosynthesis
Masuda, S., Harada, J., Yokono, M., et al. (2011) · The Plant Cell · doi:10.1105/tpc.111.085357 · Cited by 24
Trophic transfer between coastal habitats in a seagrass-dominated macrotidal embayment system as determined by stable isotope and fatty acid signatures
Park, H. J., Choy, E. J., Lee, K., & Kang, C. (2013) · Marine and Freshwater Research · doi:10.1071/mf12327 · Cited by 22
Effect of feed cycling on specific growth rate, condition factor and RNA/DNA ratio of Labeo rohita
Ali, M., Iqbal, R., Rana, S. A., et al. (2006) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.4314/ajb.v5i17.43154 · Cited by 24
Integrated analysis, transcriptome-lipidome, reveals the effects of INO- level (INO2 and INO4) on lipid metabolism in yeast
Chumnanpuen, P., Nookaew, I., & Nielsen, J. (2013) · BMC Systems Biology · doi:10.1186/1752-0509-7-s3-s7 · Cited by 24
Fatty acids influence binding of cobalt to serum albumin in patients with fatty liver
Amirtharaj, G. J., Natarajan, S. K., Mukhopadhya, A., Zachariah, U., Hegde, S. K., Kurian, G. P., Balasubramanian, K. A., & Ramachandran, A. (2008) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2008.02.006 · Cited by 25
Production of linolenic acid in yeast cells expressing an omega‐3 desaturase from tung (Aleurites fordii)
Dyer, J. M., Chapital, D. C., Kuan, J. W., et al. (2004) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-004-956-x · Cited by 24

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks