Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 24,701–24,800 of 48,846

FTY720 decreases ceramides levels in the brain and prevents memory impairments in a mouse model of familial Alzheimer’s disease expressing APOE4
Crivelli, S. M., Luo, Q., van Kruining, D., Giovagnoni, C., Mané-Damas, M., den Hoedt, S., Berkeš, D., de Vries, H. E., Mulder, M. T., Walter, J., et al. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.113240 · Cited by 23
Quality assessment and degradative changes of deep-fried oils in street fried food chain of Kashmir, India
Manzoor, S., Masoodi, F. A., Rashid, R., et al. (2022) · Food Control · doi:10.1016/j.foodcont.2022.109184 · Cited by 22
Sphingolipid Catabolism and Glycerophospholipid Levels Are Altered in Erythrocytes and Plasma from Multiple Sclerosis Patients
Momchilova, A., Pankov, R. G., Alexandrov, A., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23147592 · Cited by 22
“Lipids from erythrocyte ghosts and plasma were extracted with chloroform/methanol according to the procedure of Bligh and Dyer [59].”
Method
Characterization and cytotoxic activity of ribotoxin-like proteins from the edible mushroom Pleurotus eryngii
Landi, N., Grundner, M., Ragucci, S., et al. (2022) · Food Chemistry · doi:10.1016/j.foodchem.2022.133655 · Cited by 22
Bioremediation of dairy industry wastewater and assessment of nutrient removal potential of Chlorella vulgaris
Sudhanthiran, M. C., & Perumalsamy, M. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-03068-x · Cited by 22
Designer phospholipids – structural retrieval, chemo-/bio- synthesis and isotopic labeling
Bogojevic, O., Nygaard, J. V., Wiking, L., Arevång, C., & Guo, Z. (2022) · Biotechnology Advances · doi:10.1016/j.biotechadv.2022.108025 · Cited by 23
Portulaca oleracea L. Extract Regulates Hepatic Cholesterol Metabolism via the AMPK/MicroRNA-33/34a Pathway in Rats Fed a High-Cholesterol Diet
Jang, S., Lee, M.-S., Kang, S., Kim, C.-T., & Kim, Y. (2022) · Nutrients · doi:10.3390/nu14163330 · Cited by 22
“Hepatic and fecal lipids were extracted using the modified Bligh and Dyer method [22].”
Method
Effect of replacing a synthetic antioxidant for natural extract of yerba mate (Ilex paraguariensis) on the physicochemical characteristics, sensory properties, and gastrointestinal digestion in vitro of burgers
Feihrmann, A. C., Coutinho, F. H., dos Santos, I. C., de Marins, A. R., de Campos, T. A. F., da Silva, N. M., Duarte, V. A., Matiucci, M. A., de Souza, M. L. R., & Gomes, R. G. (2022) · Food Chemistry Advances · doi:10.1016/j.focha.2022.100130 · Cited by 22
Identification of novel halophilic/halotolerant bacterial species producing compatible solutes
Orhan, F., & Ceyran, E. (2022) · International Microbiology · doi:10.1007/s10123-022-00289-y · Cited by 22
“Afterwards, the compatible solutes were extracted from cell pellet as described previously (Bligh and Dyer 1959).”
Method
Ursolic Acid Impairs Cellular Lipid Homeostasis and Lysosomal Membrane Integrity in Breast Carcinoma Cells
Fogde, D. L., Xavier, C. P. R., Balnytė, K., Holland, L. K. K., Stahl-Meyer, K., Dinant, C., Corcelle–Termeau, E., Pereira‐Wilson, C., Maeda, K., & Jäättelä, M. (2022) · Cells · doi:10.3390/cells11244079 · Cited by 26
“The lipid extraction was performed with a modified Bligh and Dyer protocol [43].”
Method
Temperature sensitivity of soil organic carbon respiration along a forested elevation gradient in the Rwenzori Mountains, Uganda
Okello, J., Bauters, M., Verbeeck, H., Bodé, S., Kasenene, J. M., Françoys, A., Engelhardt, T., Butterbach‐Bahl, K., Kiese, R., & Boeckx, P. (2023) · Biogeosciences · doi:10.5194/bg-20-719-2023 · Cited by 25
“…was frozen immediately after collection The microbial community structure was determined using PLFA analysis The PLFA analysis was done by extracting 5 g of the freeze-dried soil sample in duplicate, following the method described by Bligh and Dyer (1959) and as modi-ﬁed by Findlay et al. (1989) .”
Impact of Regular Intake of Microalgae on Nutrient Supply and Cardiovascular Risk Factors: Results from the NovAL Intervention Study
Sandgruber, F., Höger, A.-L., Kunze, J., Schenz, B., Griehl, C., Kiehntopf, M., Kipp, K., Kühn, J., Stangl, G. I., Stefan Lorkowski, A., et al. (2023) · Nutrients · doi:10.3390/nu15071645 · Cited by 23
“Fat was extracted according to the procedure of Folch and Bligh and Dyer [24,25].”
Method
The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion
Winter, S. L., Golani, G., Lolicato, F., et al. (2023) · The EMBO Journal · doi:10.15252/embj.2023113578 · Cited by 22
Yeast-extracted nucleotides and nucleic acids as promising feed additives for European sea bass (Dicentrarchus labrax) juveniles
Pelusio, N. F., Parma, L., Volpe, E., et al. (2023) · Frontiers in Marine Science · doi:10.3389/fmars.2023.1145660 · Cited by 22
Microalgae cultivation in stabilized landfill leachate for simultaneous treatment and biomass production
Liew, L. W., Bashir, M. J. K., Toh, P. Y., Alazaiza, M. Y. D., Amr, S. S. A., Khoo, K. S., Raksasat, R., & Lim, J. W. (2023) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2023.105068 · Cited by 27
Biodiesel production and simultaneous treatment of domestic and livestock wastewater using indigenous microalgae, Chlorella sorokiniana JD1-1
Lee, J.-C., Moon, K., Lee, N., Ryu, S., Song, S. H., Kim, Y. J., Lee, S. M., Kim, H., & Joo, J.-H. (2023) · Scientific Reports · doi:10.1038/s41598-023-42453-y · Cited by 26
Differences in the phospholipid profile of melanocytes and melanoma cells irradiated with UVA and treated with cannabigerol and cannabidiol
Łuczaj, W., Dobrzyńska, I., & Skrzydlewska, E. (2023) · Scientific Reports · doi:10.1038/s41598-023-43363-9 · Cited by 22
“The Bligh and Dyer method 24 was used for total lipids extraction from cell pellet.”
Method
Effect of Oyster Mushroom Addition on Improving the Sensory Properties, Nutritional Value and Increasing the Antioxidant Potential of Carp Meat Burgers
Tokarczyk, G., Felisiak, K., Adamska, I., Przybylska, S., Hrebień-Filisińska, A. M., Biernacka, P., Bieńkiewicz, G., & Tabaszewska, M. (2023) · Molecules · doi:10.3390/molecules28196975 · Cited by 23
“Lipids were extracted using the Bligh and Dyer [56] method.”
Method
Synchronous Enhancement of Astaxanthin and Lipid Accumulation in Haematococcus Pluvialis Through Co-Mutation of Ethanol and Artp: An Exploration of Characteristics and Underlying Mechanisms
Ma, Y., Sun, X., Sun, Y., Li, H., Li, H., & Jiao, X. (2023) · Bioresource Technology · doi:10.1016/j.biortech.2024.130305 · Cited by 23
Metal accumulation in female green sea turtles (Chelonia mydas) from Eastern Atlantic affects their egg quality with potential implications for embryonic development
Morão, I. F. C., Simões, T., Casado, R. B., et al. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.172710 · Cited by 22
“Total lipids from the egg yolks were extracted and quantified using the established methodology of Bligh and Dyer (1959) with minor modifications.”
Teores de sódio e lipídios em refeições almoço consumidas por trabalhadores de uma empresa do município de Suzano, SP
Salas, C. K. T. S., Spínelli, M. G. N., Kawashima, L. M., et al. (2009) · Revista de Nutrição · doi:10.1590/s1415-52732009000300003 · Cited by 22
Impact of different extraction methods on the quality of Dipteryx alata extracts
Martins, F. S., Borges, L. L., de Paula, J. R., & da Conceição, E. C. (2013) · Revista Brasileira de Farmacognosia · doi:10.1590/s0102-695x2013005000033 · Cited by 22
Measurement of Lipid Accumulation in Chlorella vulgaris via Flow Cytometry and Liquid-State ¹H NMR Spectroscopy for Development of an NMR-Traceable Flow Cytometry Protocol
Bono, M. S., Garcia, R. D., Sri-Jayantha, D. V., Ahner, B. A., & Kirby, B. J. (2015) · PLoS ONE · doi:10.1371/journal.pone.0134846 · Cited by 23
Temperature and age affect the life history characteristics and fatty acid profiles of Moina macrocopa (Cladocera)
Flores, J. L. G., Huidobro‐Salas, M. E., Sarma, S. S. S., et al. (2015) · Journal of Thermal Biology · doi:10.1016/j.jtherbio.2015.10.005 · Cited by 22
Functional, Physicochemical and Nutritional Properties of Protein from Klunzinger's Ponyfish Extracted by the pH Shifting Method
Özyurt, G., Şimşek, A., Karakaya, B. T., et al. (2015) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.12432 · Cited by 22
Change in the Membranous Lipid Composition Accelerates Lipid Peroxidation in Young Rat Hearts Subjected to 2 Weeks of Hypoxia Followed by Hyperoxia
Oka, T., Itoi, T., Terada, N., Nakanishi, H., Taguchi, R., & Hamaoka, K. (2008) · Circulation Journal · doi:10.1253/circj.72.1359 · Cited by 22
N‐Acylated Bacteriohopanehexol‐Mannosamides from the Thermophilic Bacterium Alicyclobacillus acidoterrestris
Řezanka, T., Kyselová, L., Melzoch, K., et al. (2010) · Lipids · doi:10.1007/s11745-010-3482-4 · Cited by 22
Diallyl sulfide inhibits diethylstilbestrol induced DNA damage in human breast epithelial cells (MCF-10A)
McCaskill, M. L., Rogan, E. G., & Thomas, R. D. (2014) · Steroids · doi:10.1016/j.steroids.2014.09.005 · Cited by 22
Detection and Isolation of Antiatherogenic and Antioxidant Substances Present in Olive Mill Wastes by a Novel Filtration System
Stamatakis, G. M., Tsantila, N., Samiotaki, M. K., et al. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9016288 · Cited by 22
Mass Spectrometric Analyses of Phosphatidylcholines in Alkali-Exposed Corneal Tissue
Crane, A. M., Hua, H.-U., Coggin, A., et al. (2012) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.12-10448 · Cited by 22
Oil productivity of the tropical marine diatom Thalassiosira sp.
Nurachman, Z., Hartati, H., Anita, S., Anward, E. E., Novirani, G., Mangindaan, B., Gandasasmita, S., Syah, Y. M., Panggabean, L. M. G., & Suantika, G. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.12.082 · Cited by 22
Effect of soil moisture and bovine urine on microbial stress
Bertram, J. E., Orwin, K. H., Clough, T. J., et al. (2012) · Pedobiologia · doi:10.1016/j.pedobi.2012.03.004 · Cited by 22
Elution, Partial Separation, and Identification of Lipids Directly from Tissue Slices on Planar Chromatography Media by Desorption Electrospray Ionization Mass Spectrometry
Wiseman, J. M., & Li, J. B. (2010) · Analytical Chemistry · doi:10.1021/ac1016453 · Cited by 22
Direct and maternal n-3 long-chain polyunsaturated fatty acid supplementation improved triglyceridemia and glycemia through the regulation of hepatic and muscle sphingolipid synthesis in offspring hamsters fed a high-fat diet
Kasbi-Chadli, F., Ferchaud‐Roucher, V., Krempf, M., & Ouguerram, K. (2015) · European Journal of Nutrition · doi:10.1007/s00394-015-0879-0 · Cited by 22
Characterization of the biofuel potential of a newly isolated strain of the microalga Botryococcus braunii Kützing from Assam, India
Talukdar, J., Kalita, M. C., & Goswami, B. C. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.09.057 · Cited by 22
Scenedesmus sp. NJ-1 isolated from Antarctica: a suitable renewable lipid source for biodiesel production
Chen, Z., Gong, Y., Xian-Tao, F. A. N. G., & Hu, H. (2012) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-012-1132-0 · Cited by 22
Effect of retinol deficiency and curcumin or turmeric feeding on brain Na+?K+ adenosine triphosphatase activity
Kaul, S., & Krishnakanth, T. P. (1994) · Molecular and Cellular Biochemistry · doi:10.1007/bf00944071 · Cited by 22
Quantitative hopanoid analysis enables robust pattern detection and comparison between laboratories
Wu, C. ‐., Kong, L., Bialecka-Fornal, M., Park, S., Thompson, A. L., Kulkarni, G., Conway, S. J., & Newman, D. K. (2015) · Geobiology · doi:10.1111/gbi.12132 · Cited by 21
Quantification of bioactive sphingo- and glycerophospholipid species by electrospray ionization tandem mass spectrometry in blood
Liebisch, G., & Scherer, M. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.10.037 · Cited by 22
New dinapinone derivatives, potent inhibitors of triacylglycerol synthesis in mammalian cells, produced by Talaromyces pinophilus FKI-3864
Kawaguchi, M., Uchida, R., Ohte, S., et al. (2013) · The Journal of Antibiotics · doi:10.1038/ja.2012.127 · Cited by 22
3,5-Diiodo-L-Thyronine increases FoF1-ATP synthase activity and cardiolipin level in liver mitochondria of hypothyroid rats
Cavallo, A., Gnoni, A., Conte, E., Siculella, L., Zanotti, F., Papa, S., & Gnoni, G. V. (2011) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-011-9366-3 · Cited by 22
Age-induced perturbation in cell membrane phospholipid fatty acid profile of longevity-selected Drosophila melanogaster and corresponding control lines
Moghadam, N. N., Holmstrup, M., Pertoldi, C., & Loeschcke, V. (2013) · Experimental Gerontology · doi:10.1016/j.exger.2013.08.018 · Cited by 22
ELEVATED ENDOGENOUS SURFACTANT REDUCES INFLAMMATION IN AN ACUTE LUNG INJURY MODEL
Walker, M. G., Tessolini, J. M., McCaig, L., et al. (2009) · Experimental Lung Research · doi:10.1080/01902140902780460 · Cited by 22
Impact of diabetes mellitus on myocardial lipid deposition: An autopsy study
Nakanishi, T., & Kato, S. (2014) · Pathology - Research and Practice · doi:10.1016/j.prp.2014.04.008 · Cited by 25
Diacylglycerol Kinase Delta Promotes Lipogenesis
Shulga, Y. V., Loukov, D., Ivanova, P. T., Milne, S. B., Myers, D. S., Hatch, G. M., Umeh, G., Jalan, D., Fullerton, M. D., Steinberg, G. R., et al. (2013) · Biochemistry · doi:10.1021/bi401178y · Cited by 22
Dietary sandalwood seed oil modifies fatty acid composition of mouse adipose tissue, brain, and liver
Liu, Y. D., & Longmore, R. B. (1997) · Lipids · doi:10.1007/s11745-997-0125-x · Cited by 22
Effect of Exercise on Photoperiod-Regulated Hypothalamic Gene Expression and Peripheral Hormones in the Seasonal Dwarf Hamster Phodopus sungorus
Petri, I., Dumbell, R., Scherbarth, F., Steinlechner, S., & Barrett, P. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090253 · Cited by 21
Barramundi (Lates calcarifer) desaturase with Δ6/Δ8 dual activities
Tu, W.-C., Cook‐Johnson, R. J., James, M. J., Mühlhäusler, B. S., Stone, D. A. J., & Gibson, R. A. (2012) · Biotechnology Letters · doi:10.1007/s10529-012-0891-x · Cited by 22
Membrane-Microdomain Localization of Amyloid β-Precursor Protein (APP) C-terminal Fragments Is Regulated by Phosphorylation of the Cytoplasmic Thr668 Residue
Matsushima, T., Saito, Y., Elliott, J. I., Iijima‐Ando, K., Nishimura, M., Kimura, N., Hata, S., Yamamoto, T., Nakaya, T., & Suzuki, T. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.334847 · Cited by 22
Habitat conversion and species loss alters the composition of carbon sources to benthic communities
McLeod, R. J., Wing, S. R., & Davis, J. (2010) · Marine Ecology Progress Series · doi:10.3354/meps08677 · Cited by 22
Protein and energy nutrition of brook trout (Salvelinus fontinalis) at optimal and elevated temperatures
Amin, M. N., Carter, C. G., Barnes, R. S. K., et al. (2014) · Aquaculture Nutrition · doi:10.1111/anu.12274 · Cited by 22
Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans
Brandenburg, K., Wagner, F., Müller, M., et al. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03719.x · Cited by 22
Production of high quality gels from sea bass: Effect of MTGase and dietary fibre
Cardoso, C. L., Mendes, R. O., Vaz‐Pires, P., et al. (2011) · LWT · doi:10.1016/j.lwt.2010.12.024 · Cited by 22
rhKGF stimulates lung surfactant production in neonatal rats in vivo
Gesche, J., Fehrenbach, H., Koslowski, R., et al. (2011) · Pediatric Pulmonology · doi:10.1002/ppul.21443 · Cited by 22
Vitellogenin exists as charge isomers in the Indian freshwater murrel, Channa punctatus (Bloch)
Sehgal, N., & Goswami, S. V. (2005) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2004.10.018 · Cited by 22
A Conserved Hydrolase Responsible for the Cleavage of Aminoacylphosphatidylglycerol in the Membrane of Enterococcus faecium
Smith, A. M., Harrison, J. S., Sprague, K. M., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.484402 · Cited by 22
Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
Hosokawa, M., Sato, A., Ishigamori, H., Kohno, H., Tanaka, T., & Takahashi, K. (2001) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.2001.tb01146.x · Cited by 22
Identification of fatty acid biomarkers for quantification of neutral lipids in marine microalgae Isochrysis zhangjiangensis
Wang, H., Yao, C., Liu, Y., Meng, Y., Wang, W., Cao, X., & Xue, S. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0300-z · Cited by 22
Proportion of higher trophic-level prey in the diet of Pacific walruses (Odobenus rosmarus divergens)
Seymour, J., Horstmann-Dehn, L., & Wooller, M. J. (2014) · Polar Biology · doi:10.1007/s00300-014-1492-z · Cited by 22
Lipid and protein changes in chilled sea salmon (Pseudopercis semifasciata): effect of previous rosemary extract (Rossmarinus officinalis L.) application
Tironi, V. A., Tomás, M. C., & Añón, M. C. (2009) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2009.01955.x · Cited by 22
Studies on the production of useful chemicals, especially fatty acids in the marine diatom Nitzschia conspicua Grunow
Chu, W.-L., Phang, S., & Goh, S. (1994) · Springer eBooks · doi:10.1007/978-94-011-0958-1_4 · Cited by 22
Effects of dietary substitution of fishmeal with the combined dry microalgae,Nannochloropsis oceanica(NO) biomass residue and casein on growth and body composition of juvenile abalone (Haliotis discus)
Myung, S. H., Jung, W.-G., Kim, H. S., Kim, Y. E., Cho, S. H., Jwa, M. S., Kim, P. Y., Kim, M. K., Park, M.-W., & Kim, B.-H. (2014) · Aquaculture Research · doi:10.1111/are.12562 · Cited by 22
The effect of breed on the chemical composition and fatty acid profile of the Longissimus dorsi muscle of Brazilian beef cattle
Prado, J. M., Prado, I., Prado, I., et al. (2009) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/66387/2009 · Cited by 22
Regioisomer separation and identification of triacylglycerols containing vaccenic and oleic acids, and α- and γ-linolenic acids, in thermophilic cyanobacteria Mastigocladus laminosus and Tolypothrix sp.
Řezanka, T., Lukavský, J., Kyselová, L., & Sigler, K. (2012) · Phytochemistry · doi:10.1016/j.phytochem.2012.02.028 · Cited by 22
Effect of alternative C2 carbon sources on the growth, lipid, and γ-linolenic acid production of spirulina (Arthrospira platensis)
Golmakani, M., Rezaei, K., Mazidi, S., & Razavi, S. H. (2012) · Food Science and Biotechnology · doi:10.1007/s10068-012-0047-8 · Cited by 23
Lipidome analysis reveals antifungal polyphenol curcumin affects membrane lipid homeostasis
Sharma, M. (2011) · Frontiers in Bioscience-Elite · doi:10.2741/451 · Cited by 22
The UDP-diacylglucosamine Pyrophosphohydrolase LpxH in Lipid A Biosynthesis Utilizes Mn2+ Cluster for Catalysis
Young, H. E., Donohue, M. P., Smirnova, T. I., Smirnov, A. I., & Zhou, P. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.497636 · Cited by 22
Influence of a lipid phase on steam jet agglomeration of maltodextrin powders
Martins, P. C., & Kieckbusch, T. G. (2007) · Powder Technology · doi:10.1016/j.powtec.2007.10.022 · Cited by 22
Comparative Quality Loss in Wild and Cultured Rainbow Trout (Oncorhynchus mykiss) during Chilling Storage
Özoğul, F., Yavuzer, E., Özoğul, Y., et al. (2013) · Food Science and Technology Research · doi:10.3136/fstr.19.445 · Cited by 22
Meat quality of fully or partly outdoor reared pigs in organic production
Oksbjerg, N., Strudsholm, K., Lindahl, G., et al. (2005) · Acta Agriculturae Scandinavica Section A – Animal Science · doi:10.1080/09064700510009324 · Cited by 22
Three differently generated salmon protein hydrolysates reveal opposite effects on hepatic lipid metabolism in mice fed a high-fat diet
Vik, R., Tillander, V., Skorve, J., et al. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.03.011 · Cited by 22
Seasonal variations of fatty acid profile in different tissues of farmed bighead carp (Aristichthys nobilis)
Hong, H., Fan, H., Wang, H., et al. (2013) · Journal of Food Science and Technology · doi:10.1007/s13197-013-1129-1 · Cited by 22
Trophic structure in a pilot system for the integrated multi-trophic aquaculture off the east coast of Korean peninsula as determined by stable isotopes
Park, H. J., Han, E., Lee, W. C., et al. (2015) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2015.04.019 · Cited by 22
Effect of eight benthic diatoms as feed on the growth of red abalone (Haliotis rufescens) postlarvae
Correa-Reyes, J. G., Sánchez‐Saavedra, M. D. P., Viana, M. T., et al. (2008) · Journal of Applied Phycology · doi:10.1007/s10811-008-9381-x · Cited by 22
Hydrocarbon chain distribution of ether phospholipids of the ascidianHalocynthia roretzi and the sea urchinStrongylocentrotus intermedius
Jeong, B. Y., Ohshima, T., & Koizumi, C. (1996) · Lipids · doi:10.1007/bf02522404 · Cited by 22
Peroxisome proliferator-activated receptor α (PPARα) activation advances locomotor activity and feeding daily rhythms in mice
Gutman, R., Barnea, M., Haviv, L., Chapnik, N., & Froy, O. (2011) · International Journal of Obesity · doi:10.1038/ijo.2011.215 · Cited by 22
Serum Cytokeratin 18 M30 Antigen Level and Its Correlation with Nutritional Parameters in Middle-Aged Japanese Males with Nonalcoholic Fatty Liver Disease (NAFLD)
Tabuchi, M., Tomioka, K., Kawakami, T., MURAKAMI, Y., Hiramatsu, M., Itoshima, T., SUGAWARA, S., Kawashima, A., Okita, M., & Tsukamoto, I. (2010) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.56.271 · Cited by 22
Pair feeding-mediated changes in metabolism: stress response and pathophysiology in insulin-resistant, atherosclerosis-prone JCR:LA-cp rats
Russell, J. C., Proctor, S. D., Kelly, S. E., & Brindley, D. N. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90257.2008 · Cited by 22
Biochemical studies on the interactive effects of dietary choline and inositol in juvenile Kuruma shrimp, Marsupenaeus japonicus Bate
Michael, F. R., & Koshio, S. (2008) · Aquaculture · doi:10.1016/j.aquaculture.2008.08.006 · Cited by 22
Variation in fatty acid composition among nine forage species from a southeastern US estuarine and nearshore coastal ecosystem
Recks, M. A., & Seaborn, G. T. (2007) · Fish Physiology and Biochemistry · doi:10.1007/s10695-007-9186-x · Cited by 22
Moisture effects on microbial communities in boreal forest floors are stand-dependent
Swallow, M. J. B., & Quideau, S. A. (2012) · Applied Soil Ecology · doi:10.1016/j.apsoil.2012.09.006 · Cited by 22
Translocating southern rock lobsters (Jasus edwardsii) from deep-water to shallow inshore water enhances nutritional condition through omega-3 long-chain polyunsaturated fatty acid content
Chandrapavan, A., Guest, M. A., Nichols, P. D., & Gardner, C. (2009) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2009.04.021 · Cited by 22
Naturally occurring human plasminogen, like genetically related apolipoprotein(a), contains oxidized phosphatidylcholine adducts
Edelstein, C., Pfaffinger, D., Yang, M., Hill, J. S., & Scanu, A. M. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.03.009 · Cited by 22
Use of sugar apple, atemoya and soursop for technological development of jams: chemical and sensorial composition
Orsi, D. C., Carvalho, V. S., Nishi, A. C. F., et al. (2012) · Ciência e Agrotecnologia · doi:10.1590/s1413-70542012000500009 · Cited by 22
Biochemical composition and trophic strategies of the amphipodEurythenes gryllusat hadal depths (Atacama Trench, South Pacific)
Perrone, F. M., Croce, N. D., & Dell’Anno, A. (2003) · Chemistry and Ecology · doi:10.1080/0275754031000095723 · Cited by 22
Congelation of cryoprotective solutions and cryopreservation of fish sperm
Андреев, А. А., Садикова, Д. Г., Gakhova, E. N., et al. (2009) · BIOPHYSICS · doi:10.1134/s0006350909050108 · Cited by 22
The origin of particulate organic matter and the diet of tilapia from an estuarine ecosystem subjected to domestic wastewater discharge: fatty acid analysis approach
Sakdullah, A., & Tsuchiya, M. (2008) · Aquatic Ecology · doi:10.1007/s10452-008-9195-6 · Cited by 22
Sphingopyxis indica sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH)-contaminated dumpsite
Jindal, S., Dua, A., & Lal, R. (2012) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.040840-0 · Cited by 22
The role of progeny quality and male size in the nesting success of smallmouth bass: integrating field and laboratory studies
Gingerich, A. J., & Suski, C. D. (2011) · Aquatic Ecology · doi:10.1007/s10452-011-9371-y · Cited by 22
Light-dependent utilization of two radiolabelled carbon sources, sodium bicarbonate and sodium acetate, and relationships with long chain polyunsaturated fatty acid synthesis in the microalgaPavlova lutheri(Haptophyta)
Guihéneuf, F., Ulmann, L., Tremblin, G., & Mimouni, V. (2011) · European Journal of Phycology · doi:10.1080/09670262.2011.577912 · Cited by 22
Comparison of Methods to Reduce Dioxin and Polychlorinated Biphenyls Contents in Fishmeal: Extraction and Enzymatic Treatments
Baron, C. P., Børresen, T., & Jacobsen, C. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf061888z · Cited by 22
Shifting niches of marine predators due to human exploitation: the diet of the South American sea lion ( Otaria flavescens ) since the late Holocene as a case study
Zenteno-Devaud, L., Borella, F., Otero, J. G., et al. (2015) · Paleobiology · doi:10.1017/pab.2015.9 · Cited by 22
Isolation, identification and characterization of oleaginous fungi from the soil of Qinghai Plateau that utilize D-xylose
Li, S. (2011) · African Journal of Microbiology Research · doi:10.5897/ajmr11.171 · Cited by 24
Terriglobus albidus sp. nov., a member of the family Acidobacteriaceae isolated from Namibian semiarid savannah soil
Pascual, J., Wüst, P. K., Geppert, A. M., Foesel, B. U., Huber, K. J., & Overmann, J. (2015) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.000411 · Cited by 22
BIOCHEMICAL COMPOSITION AND ITS RELATIONSHIP WITH THE GONADAL INDEX OF THE BLACK OYSTER HYOTISSA HYOTIS (LINNAEUS, 1758) AT ESPIRITU SANTO GULF OF CALIFORNIA
Rodríguez-Astudillo, S., Villalejo-Fuerte, M. T., García-Domínguez, F., et al. (2005) · Journal of Shellfish Research · doi:10.2983/0730-8000(2005)24[975:bcairw]2.0.co;2 · Cited by 22
IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride
Berfield, A. K., Chait, A., Oram, J. F., Zager, R. A., Johnson, A. C. M., & Abrass, C. K. (2005) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00054.2005 · Cited by 21
The Effects of Varieties Sources of Omega-3 Fatty Acids on Diabetes in Rats
Soltan, S. S. A. (2012) · Food and Nutrition Sciences · doi:10.4236/fns.2012.310184 · Cited by 22
Treatment with a novel oleic-acid–dihydroxyamphetamine conjugation ameliorates non-alcoholic fatty liver disease in obese Zucker rats
Decara, J. M., Pavón, F. J., Suárez, J., et al. (2015) · Disease Models & Mechanisms · doi:10.1242/dmm.019919 · Cited by 22
Antiobese function of platelet‐activating factor: increased adiposity in platelet‐activating factor receptor‐deficient mice with age
Sugatani, J., Sadamitsu, S., Yamaguchi, M., Yamazaki, Y., Higa, R., Hattori, Y., Uchida, T., Ikari, A., Sugiyama, W., Watanabe, T., et al. (2013) · The FASEB Journal · doi:10.1096/fj.13-233262 · Cited by 22
Simultaneous determination of natural‐abundance δ 15 N values and quantities of individual amino acids in proteins from milk of lactating women and from infant hair using gas chromatography/isotope ratio mass spectrometry
Tea, I., Guennec, A. L., Frasquet-Darrieux, M., et al. (2013) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.6582 · Cited by 22

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks