Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides
Abstract
Detailed studies on the enzyme machinery responsible for the biosynthesis of protein-bound oligosaccharides of the Asn-GlcNAc and Ser(Thr)-GalNAc linkage types have allowed the formulation of some general rules which explain, at least in part, the branching patterns and microheterogeneity of these structures. These rules are discussed under the following headings: competition of two or more enzymes for a common substrate; controls at the level of enzyme substrate specificity (e.g., critical sugar residues which turn enzyme activity on or off, branch specificity, and the role of the polypeptide in the glycoprotein substrate); substrate availability.
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The non-templated biosynthesis of glycans produces heterogeneous glycomic profiles due to enzymatic competition for a common substrate, substrate availability and enzyme-substrate specificity.
“the non-templated biosynthesis of glycans is a major hurdle that produces heterogeneous glycomic profiles as a result of enzymatic competition for a common substrate, substrate availability and enzyme-substrate specificity [6,7].”
From Glycoengineering Chinese hamster ovary cells: a short history (2021) · cites this paper as [6]
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References 59 · Cited by 526
- Authors
- Schachter, H.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Schachter, Harry
- Venue
- Biochemistry and Cell Biology
- Publisher
- Canadian Science Publishing
- Published
- 1986-03-01
- Volume
- 64
- Issue
- 3
- Pages
- 163-181
- ISSN
- 0829-8211 (ISSN-L)
- 1208-6002
- OpenAlex
- W2013796022
- Cited by
- 526
- References
- 59
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 5 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 8 · 2019: 7 · 2018: 13 · 2017: 15 · 2016: 7 · 2015: 16 · 2014: 12 · 2013: 2 · 2012: 15
- FWCI
- 6.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry and Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- glycosylation, N-glycosylation, O-glycosylation, branching pattern, microheterogeneity, enzyme substrate specificity, substrate availability, enzyme competition, glycoproteins
- MeSH terms
- Animals, Blood Group Antigens, Carbohydrate Conformation, Carbohydrate Sequence, Glycoproteins, Humans, Models, Molecular, Oligosaccharides, Organ Specificity, Polymorphism, Genetic, Species Specificity
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