Inhibition of Inflammatory Responses by Centella asiatica via Suppression of IRAK1-TAK1 in Mouse Macrophages
Abstract
Centella asiatica (L.) Urb. (C. asiatica) has been widely treated for inflammation-related diseases in China for thousands of years. While C. asiatica showed relevant effects as traditional medicine, the mechanism of C. asiatica suppressing inflammation has not been thoroughly investigated. Therefore, this study was conducted to reveal the anti-inflammatory mechanism of methanol fraction from C. asiatica (MCA) at the molecular level in murine macrophages. Levels of inflammation-related mediators were observed with treatment of MCA. MCA significantly suppressed nitric oxide production and iNOS expression in RAW 264.7 macrophages. Prostaglandin E2production was alleviated by MCA via the downregulation of cyclooxygenase-2. MCA treatment also reduced pro-inflammatory tumor necrosis factor-[Formula: see text] and interleukin (IL)-6 levels. LPS/D-GalN-induced acute hepatitis in mouse was alleviated by MCA treatment. In addition, MCA decreased the phosphorylation of inhibitory [Formula: see text]B[Formula: see text] (I[Formula: see text]B[Formula: see text]) at Ser32/36 and thereby blocked I[Formula: see text]B[Formula: see text] degradation. TXY motif phosphorylation in the activation loops of mitogen-activated protein kinases (MAPKs) was also suppressed by MCA treatment. Further investigation revealed that MCA inhibited transforming growth factor-[Formula: see text]-activated kinase 1 (TAK1) phosphorylation and IL-1 receptor-associated kinase (IRAK1) degradation, the upstream kinases activating nuclear factor [Formula: see text]B and MAPKs. Taken together, MCA exhibited anti-inflammatory properties via the downregulation of IRAK1-TAK1 signaling pathways.
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Cho et al. reported that C. asiatica extract inhibited inflammatory responses in LPS-stimulated macrophages by downregulating the IRAK1-TAK1 signaling axis.
“Cho et al. [14] reported that C. asiatica extract inhibited inflammatory responses in LPS-stimulated macrophages by downregulating the IRAK1-TAK1 signaling axis.”
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References 41 · Cited by 15
- Authors
- Cho, Y.-C., Vuong, H. L., Ha, J., Lee, S., Park, J., Wibow, A. E., & Cho, S.
- Affiliations
- Chonnam National University (KR)
- Chung-Ang University (KR)
- Badan Pengkajian dan Penerapan Teknologi (ID)
- Venue
- The American Journal of Chinese Medicine
- Publisher
- World Scientific Pub Co Pte Ltd
- Published
- 2020-01
- Volume
- 48
- Issue
- 05
- Pages
- 1103-1120
- ISSN
- 0090-2942 (ISSN-L)
- 0192-415X
- 1793-6853
- OpenAlex
- W3042275146
- Cited by
- 15
- References
- 41
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 1
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The American Journal of Chinese Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (NRF) grant funded by the Ministry of Education (NRF-2016R1A6A3A11931134)
- Ministry of Science and ICT (NRF-2018R1A2B6005084, 2015R1A5A1008958)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Keywords
- Centella asiatica, methanol fraction, anti-inflammatory mechanism, IRAK1, TAK1, NF-κB, MAPK activation, macrophages, nitric oxide, cyclooxygenase-2, prostaglandin E2, TNF-α, interleukin-6, acute hepatitis, traditional Chinese medicine
- MeSH terms
- RAW 264.7 Cells, MAP Kinase Kinase Kinase 7, Animals, Anti-Inflammatory Agents, Macrophages, Male, Mice, Inbred C57BL, Phosphorylation, Down-Regulation, Interleukin-6, Gene Expression, Inflammation Mediators, Mitogen-Activated Protein Kinases, MAP Kinase Kinase Kinases, Centella, Mice, Interleukin-1 Receptor-Associated Kinases
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