Imaging Hypoxia in Orthotopic Rat Liver Tumors with Iodine 124–labeled Iodoazomycin Galactopyranoside PET
Open accessAbstract
Purpose To evaluate iodine 124 (124I)-labeled iodoazomycin galactopyranoside (IAZGP) positron emission tomography (PET) in the detection of hypoxia in an orthotopic rat liver tumor model by comparing regions of high (124)I-IAZGP uptake with independent measures of hypoxia and to determine the optimal time after injection to depict hypoxia. Materials and methods The institutional animal care and use committee approved this study. Morris hepatoma tumors were established in the livers of 15 rats. Tumor oxygenation was measured in two rats with a fluorescence fiberoptic oxygen probe. (124)I-IAZGP was coadministered with the established hypoxia markers pimonidazole and EF5 in nine rats; 12-hour PET data acquisition was performed 24 hours later. Tumor cryosections were analyzed with immunofluorescence and autoradiography. In the four remaining rats, serial 20- and 60-minute PET data acquisition was peformed up to 48 hours after tracer administration. Results Oxygen probe measurements showed severe hypoxia ( Conclusion Regions of high (124)I-IAZGP uptake in orthotopic rat liver tumors are consistent with independent measures of hypoxia; visualization of hypoxia with (124)I-IAZGP PET is optimal 6 hours after injection.
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In orthotopic rat HCC tumors, median PO2 values ranged from 0.2 to 0.8 mmHg compared with 45 mmHg in normal rat liver.
“Analysis of orthotopic rat HCC tumors revealed that median PO2 values ranged from 0.2 to 0.8 mmHg, as compared with 45 mmHg in normal rat liver (21).”
From HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy (2022) · cites this paper as [21]
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Koukourakis, M. I., Giatromanolaki, A., Sivridis, E., Simopoulos, C., Turley, H., Talks, K., Gatter, K. C., & Harris, A. L. (2002) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/s0360-3016(02)02848-1 · Cited by 361 · see citing works
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Salman, S., Meyers, D. J., Wicks, E. E., Lee, S. N., Datan, E., Thomas, A. M., Anders, N. M., Hwang, Y., Lyu, Y., Yang, Y., et al. (2022) · Journal of Clinical Investigation · doi:10.1172/jci156774 · Cited by 111 · see citing works
- Authors
- Riedl, C. C., Brader, P., Zanzonico, P. B., Chun, Y. S., Woo, Y., Singh, P., Carlin, S., Wen, B., Ling, C. C., Hricak, H., & Fong, Y.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- ORCID
- Venue
- Radiology
- Published
- 2008-08
- Volume
- 248
- Issue
- 2
- Pages
- 561-570
- ISSN
- 0033-8419 (ISSN-L)
- 1527-1315
- PMC
- PMC2797648
- OpenAlex
- W2027823530
- Cited by
- 41
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 2 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 1 · 2013: 4 · 2012: 1
- FWCI
- 1.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Radiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Pennsylvania
- Memorial Sloan-Kettering Cancer Center
- National Institutes of Health (R25-CA096945)
- National Cancer Institute (R01 CA 75416, R25CA096945, R25-CA096945-3, R01CA075416)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Radiopharmaceutical Chemistry and Applications
- Keywords
- tumor hypoxia, iodine-124, positron emission tomography, liver tumors, pimonidazole, EF5, tumor oxygenation, hypoxia imaging
- MeSH terms
- Animals, Hypoxia, Carcinoma, Hepatocellular, Image Processing, Computer-Assisted, Iodine Radioisotopes, Liver Neoplasms, Monosaccharides, Nitroimidazoles, Rats, Nude, Positron-Emission Tomography, Rats
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