Is it time to reinvent basic cell culture medium?
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The electrolyte composition and carbohydrate content of these types of medium are usually not ideal physiological environments.
“The fact that the electrolyte composition and carbohydrate content of these types of medium are usually not ideal physiological environments is often overlooked.”
From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as McKee and Komarova, 2017
Approximately 90% of all reported in vitro experiments are currently conducted with one of four different types of medium: DMEM, RPMI, M199, MEM.
“Approximately 90% of all reported in vitro experiments are currently conducted with one of four different types of medium: DMEM, RPMI, M199; MEM (McKee and Komarova, 2017).”
From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as McKee and Komarova, 2017
A typical scenario resulting from the use of these media is an oversupply of phosphate and/or a limitation of calcium and magnesium.
“A typical scenario resulting from the use of these media is an oversupply of phosphate and/or a limitation of calcium and magnesium (McKee and Komarova, 2017).”
From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as McKee and Komarova, 2017
Cites
Raise standards for preclinical cancer research
Begley, C. G., & Ellis, L. M. (2012) · Nature · doi:10.1038/483531a · Cited by 2,979 · cited 2× · see citing works
“In conclusion, we suggest that the nonphysiological electrolyte and carbohydrate microenvironment of the most commonly used cell culture media may result in unintended and uncontrolled changes in cell behavior, thereby contributing to the difficulties in reproducibility observed in modern publications (4).”
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In Vitro research Reproducibility: Keeping up High Standards
Hirsch, C., & Schildknecht, S. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.01484 · Cited by 161 · see citing works
- Authors
- McKee, T. J., & Komarova, S. V.
- Affiliations
- Shriners Hospitals for Children - Canada (CA)
- Corresponding author
- Komarova, Svetlana V.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2017-05-01
- Volume
- 312
- Issue
- 5
- Pages
- C624-C626
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2590600856
- Cited by
- 85
- References
- 1
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 13 · 2023: 10 · 2022: 13 · 2021: 10 · 2020: 12 · 2019: 8 · 2018: 3
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Canadian Institutes for Health Research (77643)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- cell culture media, culture medium composition, cell growth
- MeSH terms
- Animals, Culture Media, Glucose, Humans, Metals, Cell Culture Techniques
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