The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A practical guide to evaluating colocalization in biological microscopy
Open accessAbstract
Fluorescence microscopy is one of the most powerful tools for elucidating the cellular functions of proteins and other molecules. In many cases, the function of a molecule can be inferred from its association with specific intracellular compartments or molecular complexes, which is typically determined by comparing the distribution of a fluorescently labeled version of the molecule with that of a second, complementarily labeled probe. Although arguably the most common application of fluorescence microscopy in biomedical research, studies evaluating the “colocalization” of two probes are seldom quantified, despite a diversity of image analysis tools that have been specifically developed for that purpose. Here we provide a guide to analyzing colocalization in cell biology studies, emphasizing practical application of quantitative tools that are now widely available in commercial and free image analysis software.
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Burette, A. C., Strehler, E. E., & Weinberg, R. J. (2009) · Journal of Comparative Neurology · doi:10.1002/cne.21909 · Cited by 40
Cited by
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Chung, K., Wallace, J., Kim, S.-Y., Kalyanasundaram, S., Andalman, A. S., Davidson, T. J., Mirzabekov, J. J., Zalocusky, K. A., Mattis, J., Denisin, A. K., et al. (2013) · Nature · doi:10.1038/nature12107 · Cited by 2,008
Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons
Cheng, X.-T., Xie, Y.-X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z.-H. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201711083 · Cited by 294
Mitochondria are secreted in extracellular vesicles when lysosomal function is impaired
Liang, W., Sagar, S., Ravindran, R., Najor, R. H., Quiles, J. M., Chi, L., Diao, R. Y., Woodall, B. P., Leon, L. J., Zumaya, E., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-40680-5 · Cited by 273
Meilandt, W. J., Ngu, H., Gogineni, A., Lalehzadeh, G., Lee, S.-H., Srinivasan, K., Imperio, J., Wu, T., Weber, M., Kruse, A. J., et al. (2020) · The Journal of Neuroscience · doi:10.1523/jneurosci.1871-19.2019 · Cited by 210
“To determine colocalization of ApoE and Iba1 staining, or methoxy-X04 and Iba1 staining, we calculated the Mander’s colocalization coefficients using the ImageJ plugin JACoP, as described previously (Dunn et al., 2011).”
MethodLysosomal recruitment of TSC2 is a universal response to cellular stress
Demetriades, C., Plescher, M., & Teleman, A. A. (2016) · Nature Communications · doi:10.1038/ncomms10662 · Cited by 160
Metadata
- Authors
- Dunn, K. W., Kamocka, M. M., & McDonald, J. H.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2011-04
- Volume
- 300
- Issue
- 4
- Pages
- C723-C742
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2150737519
- Cited by
- 2,038
- References
- 1
- FWCI
- 17.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
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