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Cited by 46Sperm and Testicular FunctionMicrotubule and mitosis dynamicsReproductive Biology and Fertility

Dynein 1 supports spermatid transport and spermiation during spermatogenesis in the rat testis

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Wen, Qing, Tang, Elizabeth I., Lui, Wing-yee, Lee, Will M., Wong, Chris K. C., Silvestrini, Bruno, Cheng, C. Yan·2018·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00114.2018PMC PMC6293164
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Abstract

In the mammalian testis, spermatogenesis is dependent on the microtubule (MT)-specific motor proteins, such as dynein 1, that serve as the engine to support germ cell and organelle transport across the seminiferous epithelium at different stages of the epithelial cycle. Yet the underlying molecular mechanism(s) that support this series of cellular events remain unknown. Herein, we used RNAi to knockdown cytoplasmic dynein 1 heavy chain (Dync1h1) and an inhibitor ciliobrevin D to inactivate dynein in Sertoli cells in vitro and the testis in vivo, thereby probing the role of dynein 1 in spermatogenesis. Both treatments were shown to extensively induce disruption of MT organization across Sertoli cells in vitro and the testis in vivo. These changes also perturbed the transport of spermatids and other organelles (such as phagosomes) across the epithelium. These changes thus led to disruption of spermatogenesis. Interestingly, the knockdown of dynein 1 or its inactivation by ciliobrevin D also perturbed gross disruption of F-actin across the Sertoli cells in vitro and the seminiferous epithelium in vivo, illustrating there are cross talks between the two cytoskeletons in the testis. In summary, these findings confirm the role of cytoplasmic dynein 1 to support the transport of spermatids and organelles across the seminiferous epithelium during the epithelial cycle of spermatogenesis.

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  • The shRNA for rat cytoplasmic DHC1 has been reported to reduce DHC1 in cultured rat Sertoli cells.

    “We used a shRNA for rat cytoplasmic DHC1, which has been reported to reduce it in cultured rat Sertoli cells (29) (Fig. 6C).”

    From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [29]

    The Materials and Methods say the authors transfected cultured rat Sertoli cells with Dync1h1-specific siRNA duplexes, not shRNA. The Results report that this RNAi reduced Dync1h1 expression by about 70% in those cells. The reduction is supported, but the claim identifies the wrong reagent. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Wen, Q., Tang, E. I., Lui, W.-Y., Lee, W. M., Wong, C. K. C., Silvestrini, B., & Cheng, C. Y.
Affiliations
  • Population Council (US)
  • University of Hong Kong (HK)
  • Hong Kong Baptist University (HK)
  • SBM Srl Pharmaceuticals, Rome, Italy
Corresponding author
Cheng, C. Yan
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2018-11-01
Volume
315
Issue
5
Pages
E924-E948
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00114.2018
PMC
PMC6293164
OpenAlex
W2884472498
Cited by
46
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 7 · 2019: 7 · 2018: 1
FWCI
5.90
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
HHS | NIH | National Institute of Child Health and Human Development (NICHD) (R01 HD56034, U54 HD029990 Project 5)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
cytoplasmic dynein, DYNC1H1, spermatogenesis, spermiation, Sertoli cells, microtubule motor proteins, microtubule organization, F-actin, organelle transport, seminiferous epithelium, testis
MeSH terms
Animals, Biological Transport, Dyneins, Male, Sertoli Cells, Spermatids, Spermatogenesis, Testis, RNA Interference, Rats, Quinazolinones

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