Measurement of interstitial insulin in human adipose and muscle tissue under moderate hyperinsulinemia by means of direct interstitial access
Abstract
Insulin's action to stimulate glucose utilization is determined by the insulin concentration in interstitial fluid (ISF) of insulin-sensitive tissues. The concentration of interstitial insulin has been measured in human subcutaneous adipose tissue and skeletal muscle, however, never in parallel. The aim of this study was to compare interstitial insulin levels between both tissue beds by simultaneous measurements and to verify and quantify low peripheral ISF insulin fractions as found during moderate hyperinsulinemia. Nine healthy subjects (27.2 +/- 0.8 yr) were investigated. A euglycemic-hyperinsulinemic clamp was started with a primed-constant intravenous insulin infusion of 1 mU x kg(-1) x min(-1). For direct access to ISF, macroscopically perforated open-flow microperfusion catheters were inserted in both tissues. During steady-state conditions (9.5 h), interstitial effluents were collected in 30-min fractions using five different insulin concentrations in the inflowing perfusates ("no net flux" protocol). Regression analysis of insulin concentrations in perfusates and effluents yielded the relative recovery and the perfusate insulin concentration, which was in equilibrium with the surrounding tissue. Thus, in subcutaneous adipose tissue and skeletal muscle, the mean ISF-to-serum insulin level was calculated as 21.0% [95% confidence interval (CI) 17.5-24.5] and 26.0% (95% CI 19.1-32.8; P = 0.14), respectively. Recoveries for insulin averaged 51 and 64%, respectively. The d…
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The action of insulin necessary to stimulate peripheral glucose utilization is determined by its concentration in interstitial fluid that bathes insulin-sensitive cells.
“The action of insulin necessary to stimulate peripheral glucose utilization is also determined by its concentration in interstitial fluid that bathes insulin-sensitive cells [27].”
From A mathematical model of brain glucose homeostasis (2009) · cites this paper as [27]
The uptake of glucose peripherally depends not only on local glucose concentration but also on local insulin concentration.
“The uptake of glucose peripherally (primarily the muscle, gut, lungs, liver, pancreas, kidneys and the other mass) depends not only on local glucose concentration but also on local insulin concentration.”
From A mathematical model of brain glucose homeostasis (2009) · cites this paper as [27]
Insulin concentrations in various extracranial mass compartments are assumed on account of mass-to-blood insulin level of about 25%.
“Insulin concentrations in various extracranial mass compartments are assumed on account of mass-to-blood insulin level of about 25% [27].”
From A mathematical model of brain glucose homeostasis (2009) · cites this paper as [27]
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References 24 · Cited by 57
- Authors
- Bodenlenz, M., Schaupp, L. A., Druml, T., Sommer, R., Wutte, A., Schaller, H. C., Sinner, F., Wach, P., & Pieber, T. R.
- Affiliations
- Medical University of Graz (AT)
- Graz University of Technology (AT)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2005-08
- Volume
- 289
- Issue
- 2
- Pages
- E296-E300
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2107098584
- Cited by
- 57
- References
- 24
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 7 · 2013: 5 · 2012: 6
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Innovációs és Technológiai Minisztérium
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- interstitial fluid, hyperinsulinemia, subcutaneous adipose tissue, skeletal muscle, hyperinsulinemic-euglycemic clamp, insulin-sensitive tissues
- MeSH terms
- Adipose Tissue, Adult, Humans, Insulin, Reference Values, Glucose Clamp Technique, Muscle, Skeletal, Extracellular Fluid
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