The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Fat feeding causes widespread in vivo insulin resistance, decreased energy expenditure, and obesity in rats
Abstract
High levels of dietary fat may contribute to both insulin resistance and obesity in humans but evidence is limited. The euglycemic clamp technique combined with tracer administration was used to study insulin action in vivo in liver and individual peripheral tissues after fat feeding. Basal and nutrient-stimulated metabolic rate was assessed by open-circuit respirometry. Adult male rats were pair-fed isocaloric diets high in either carbohydrate (69% of calories; HiCHO) or fat (59% of calories; HiFAT) for 24 +/- 1 days. Feeding of the HiFAT diet resulted in a greater than 50% reduction in net whole-body glucose utilization at midphysiological insulin levels (90-100 mU/l) due to both reduced glucose disposal and, to a lesser extent, failure to suppress liver glucose output. Major suppressive effects of the HiFAT diet on glucose uptake were found in oxidative skeletal muscles (29-61%) and in brown adipose tissue (BAT; 78-90%), the latter accounting for over 20% of the whole-body effect. There was no difference in basal metabolic rate but thermogenesis in response to glucose ingestion was higher in the HiCHO group. In contrast to their reduced BAT weight, the HiFAT group accumulated more white adipose tissue, consistent with reduced energy expenditure. HiFAT feeding also resulted in major decreases in basal and insulin-stimulated conversion of glucose to lipid in liver (26-60%) and brown adipose tissue (88-90%) with relatively less effect in white adipose (0-43%). We conclude that high-fat feeding results in insulin resistance due mainly to effects in oxidative skeletal muscle and BAT.(ABSTRACT TRUNCATED AT 250 WORDS)
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Cites
New methods for calculating metabolic rate with special reference to protein metabolism
Weir, J. B. D. V. (1949) · The Journal of Physiology · doi:10.1113/jphysiol.1949.sp004363 · Cited by 5,603
HINDLIMB MUSCLE FIBER POPULATIONS OF FIVE MAMMALS
ARIANO, M. A., EDGERTON, V. R., & ARMSTRONG, R. B. (1973) · Journal of Histochemistry & Cytochemistry · doi:10.1177/21.1.51 · Cited by 1,129
Heterogeneity of insulin action in individual muscles in vivo: euglycemic clamp studies in rats
James, D. E., Jenkins, A. B., & Kraegen, E. W. (1985) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1985.248.5.e567 · Cited by 321
Self-citationNutritional influences on lipogenesis and thermogenesis after a carbohydrate meal
Acheson, K. J., Schutz, Y., Bessard, T., Ravussin, E., Jequier, E., & Flatt, J. P. (1984) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1984.246.1.e62 · Cited by 250
Fatty Acid and 3-β-Hydroxysterol Synthesis in the Perfused Rat Liver
Brunengraber, H., Boutry, M., & Lowenstein, J. M. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44057-x · Cited by 213
Danforth, E. (1985) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/41.5.1132 · Cited by 197
Grundleger, M. L., & Thenen, S. W. (1982) · Diabetes · doi:10.2337/diab.31.3.232 · Cited by 161
In vivo insulin sensitivity in the rat determined by euglycemic clamp
Kraegen, E. W., James, D. E., Bennett, S. P., & Chisholm, D. J. (1983) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1983.245.1.e1 · Cited by 153
Self-citationEffects of exercise training on in vivo insulin action in individual tissues of the rat.
James, D. E., Kraegen, E. W., & Chisholm, D. J. (1985) · Journal of Clinical Investigation · doi:10.1172/jci112019 · Cited by 146
Self-citationRole of palatability on meal-induced thermogenesis in human subjects
LEBLANC, J., & Brondel, L. (1985) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1985.248.3.e333 · Cited by 117
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Brown Adipose Tissue: Function and Physiological Significance
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Srinivasan, K., Viswanad, B., Asrat, L., Kaul, C. L., & Ramarao, P. (2005) · Pharmacological Research · doi:10.1016/j.phrs.2005.05.004 · Cited by 1,874
Liu, S., Willett, W. C., Manson, J. E., Hu, F. B., Rosner, B., & Colditz, G. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.5.920 · Cited by 787
High-fat diets cause insulin resistance despite an increase in muscle mitochondria
Hancock, C. R., Han, D.-H., Chen, M., Terada, S., Yasuda, T., Wright, D. C., & Holloszy, J. O. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0802057105 · Cited by 538
Adaptation and Maladaptation of the Heart in Diabetes: Part I: General Concepts
Taegtmeyer, H., McNulty, P., & Young, M. E. (2002) · Circulation · doi:10.1161/01.cir.0000012466.50373.e8 · Cited by 521
Metadata
- Authors
- Storlien, L. H., James, D. E., Burleigh, K. M., Chisholm, D. J., & Kraegen, E. W.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 1986-11-01
- Volume
- 251
- Issue
- 5
- Pages
- E576-E583
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W1959312622
- Cited by
- 458
- References
- 23
- FWCI
- 3.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- Storlien, L. H., James, D. E., Burleigh, K. M., Chisholm, D. J., & Kraegen, E. W. (1986). Fat feeding causes widespread in vivo insulin resistance, decreased energy expenditure, and obesity in rats. American Journal of Physiology-Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.1986.251.5.e576
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