Effects of individual fatty acids on glucose uptake and glycogen synthesis in soleus muscle in vitro
Open accessAbstract
Soleus muscle strips from Wistar rats were preincubated with palmitate in vitro before the determination of insulin-mediated glucose metabolism in fatty acid-free medium. Palmitate decreased insulin-stimulated glycogen synthesis to 51% of control in a time- (0–6 h) and concentration-dependent (0–2 mM) manner. Basal and insulin-stimulated glucose transport/phosphorylation also decreased with time, but the decrease occurred after the effect on glycogen synthesis. Preincubation with 1 mM palmitate, oleate, linoleate, or linolenate for 4 h impaired glycogen synthesis stimulated with a submaximal physiological insulin concentration (300 μU/ml) to 50–60% of the control response, and this reduction was associated with impaired insulin-stimulated phosphorylation of protein kinase B (PKB). Preincubation with different fatty acids (all 1 mM for 4 h) had varying effects on insulin-stimulated glucose transport/phosphorylation, which was decreased by oleate and linoleate, whereas palmitate and linolenate had little effect. Across groups, the rates of glucose transport/phosphorylation correlated with the intramuscular long-chain acyl-CoA content. The similar effects of individual fatty acids on glycogen synthesis but different effects on insulin-stimulated glucose transport/phosphorylation provide evidence that lipids may interact with these two pathways via different mechanisms.
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Exposure to NEFA in vitro decreased insulin-mediated glucose uptake and glycogen synthesis and reduced beta-cell function, including reduced insulin release and impaired proinsulin to insulin processing.
“Exposure to NEFA in vitro decreased insulin-mediated glucose uptake and glycogen synthesis [146] as well as reduced beta-cell function [147, 148], encompassing both reduced insulin release and impaired proinsulin to insulin processing.”
From The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of type 2 diabetes (2003) · cites this paper as [146]
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References 44 · Cited by 107
- Authors
- Thompson, A. L., Lim-Fraser, M. Y.-C., Kraegen, E. W., & Cooney, G. J.
- Affiliations
- Garvan Institute of Medical Research (AU)
- ORCID
- Cooney, Gregory J.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2000-09-01
- Volume
- 279
- Issue
- 3
- Pages
- E577-E584
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W1778141833
- Cited by
- 107
- References
- 44
- Citations by year
- 2025: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 4
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Royal Society
- University of New South Wales
- Medical Research Council
- National Health and Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- fatty acids, palmitate, oleate, linoleate, linolenic acid, insulin-stimulated glucose uptake, glucose transport, glucose phosphorylation, glycogen synthesis, Akt, soleus muscle, long-chain acyl-CoA, skeletal muscle glucose metabolism
- MeSH terms
- Acyl Coenzyme A, Adenosine Triphosphate, Animals, Dietary Fats, Fatty Acids, Glucose, Glycogen, Insulin, Insulin Resistance, Male, Monosaccharide Transport Proteins, Proto-Oncogene Proteins, Rats, Wistar, Protein Serine-Threonine Kinases, Muscle, Skeletal, Glucose-6-Phosphate, Proto-Oncogene Proteins c-akt, Rats, In Vitro Techniques
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