Identification of a basolateral Cl−/HCO 3 − exchanger specific to gastric parietal cells
Abstract
The basolateral Cl(-)/HCO(3)(-) exchanger in parietal cells plays an essential role in gastric acid secretion mediated via the apical gastric H(+)-K(+)-ATPase. Here, we report the identification of a new Cl(-)/HCO(3)(-) exchanger, which shows exclusive expression in mouse stomach and kidney, with expression in the stomach limited to the basolateral membrane of gastric parietal cells. Tissue distribution studies by RT-PCR and Northern hybridizations demonstrated the exclusive expression of this transporter, also known as SLC26A7, to stomach and kidney, with the stomach expression significantly more abundant. No expression was detected in the intestine. Cellular distribution studies by RT-PCR and Northern hybridizations demonstrated predominant localization of SLC26A7 in gastric parietal cells. Immunofluorescence labeling localized this exchanger exclusively to the basolateral membrane of gastric parietal cells, and functional studies in oocytes indicated that SLC26A7 is a DIDS-sensitive Cl(-)/HCO(3)(-) exchanger that is active in both acidic and alkaline pH(i). On the basis of its unique expression pattern and function, we propose that SLC26A7 is a basolateral Cl(-)/HCO(3)(-) exchanger in gastric parietal cells and plays a major role in gastric acid secretion.
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Bicarbonate ions are transported into the blood through the stomach.
“Bicarbonate ions are known to be transported into the blood through the stomach [38,39].”
From Effects of Enteric-Coated Formulation of Sodium Bicarbonate on Bicarbonate Absorption and Gastrointestinal Discomfort (2024) · cites this paper as [38]
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Homozygous loss-of-function mutations in SLC26A7 cause goitrous congenital hypothyroidism
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Self-citationJiang, F.-L., Jeong, D.-H., Eom, S.-H., Lee, H.-M., Cha, B.-J., Park, J.-S., Kwon, R., Nam, J.-Y., Yu, H.-S., Heo, S.-H., et al. (2024) · Nutrients · doi:10.3390/nu16050744 · Cited by 4 · see citing works
- Authors
- Petrovic, S., Ju, X., Barone, S., Seidler, U., Alper, S. L., Lohi, H., Kere, J., & Soleimani, M.
- Affiliations
- Department of Medicine, University of Cincinnati, Cincinnati 45267;
- University of Cincinnati (US)
- University of Tübingen (DE)
- Harvard University (US)
- University of Helsinki (FI)
- Karolinska Institutet (SE)
- Veterans Health Administration (US)
- Venue
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Publisher
- American Physiological Society
- Published
- 2003-06-01
- Volume
- 284
- Issue
- 6
- Pages
- G1093-G1103
- ISSN
- 0193-1857 (ISSN-L)
- 1522-1547
- OpenAlex
- W2070295373
- Cited by
- 91
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 4 · 2021: 5 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 5 · 2012: 2
- FWCI
- 1.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk054430-04, 5r01dk052820-04)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK52820, DK 54430)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- SLC26A7, Cl-/HCO3- exchanger, basolateral membrane, parietal cells, gastric acid secretion, H+/K+-ATPase, intracellular pH, kidney, stomach, ion transporters
- MeSH terms
- Sulfate Transporters, Animals, Fluorescent Antibody Technique, Gastric Acid, Humans, Hydrogen-Ion Concentration, Kidney, Organ Specificity, Parietal Cells, Gastric, RNA, Messenger, Blotting, Western, Gene Expression, Antiporters, Chloride-Bicarbonate Antiporters, Mice
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