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miR-221 suppresses ICAM-1 translation and regulates interferon-γ-induced ICAM-1 expression in human cholangiocytes

Hu, Guoku, Gong, Ai-Yu, Liu, Jun, Zhou, Rui, Deng, Caishu, Chen, Xian-Ming·2010·American Journal of Physiology-Gastrointestinal and Liver Physiology
DOI 10.1152/ajpgi.00490.2009
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Abstract

Aberrant cholangiocyte reactions in response to inflammatory stimuli are important pathogenic factors for the persistent biliary inflammation in patients with cholangiopathies. Overexpression of intercellular cell adhesion molecule-1 (ICAM-1) in cholangiocytes is a common pathological feature in inflammatory cholangiopathies and can promote cholangiocyte interactions with effector lymphocytes in the portal region. In this study, we tested the involvement of miRNA-mediated posttranscriptional regulation in IFN-γ-induced ICAM-1 expression in cholangiocytes. Using both immortalized and nonimmortalized human cholangiocyte cell lines, we found that IFN-γ activated ICAM-1 transcription and increased ICAM-1 protein expression. Inhibition of ICAM-1 transcription could only partially block IFN-γ-induced ICAM-1 expression at the protein level. In silico target prediction analysis revealed complementarity of miR-221 to the 3′-untranslated region of ICAM-1 mRNA. Targeting of ICAM-1 3′-untranslated region by miR-221 resulted in translational repression in cholangiocytes but not ICAM-1 mRNA degradation. Functional inhibition of miR-221 with anti-miR-221 induced ICAM-1 protein expression. Moreover, IFN-γ stimulation decreased miR-221 expression in cholangiocytes in a signal transducer and activator of transcription 1-dependent manner. Transfection of miR-221 precursor abolished IFN-γ-stimulated ICAM-1 protein expression. In addition, miR-221-mediated expression of ICAM-1 on cholangiocytes showed a significant influence on the adherence of cocultured T cells. These findings indicate that both transcriptional and miRNA-mediated posttranscriptional mechanisms are involved in IFN-γ-induced ICAM-1 expression in human cholangiocytes, suggesting an important role for miRNAs in the regulation of cholangiocyte inflammatory responses.

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Cited by

Sorted by citations

Authors
Hu, G., Gong, A.-Y., Liu, J., Zhou, R., Deng, C., & Chen, X.-M.
Affiliations
  • Departments of 1Medical Microbiology and Immunology and
  • Pathology, Creighton University Medical Center, Omaha, Nebraska
Venue
American Journal of Physiology-Gastrointestinal and Liver Physiology
Publisher
American Physiological Society
Published
2010-04
Volume
298
Issue
4
Pages
G542-G550
ISSN
  • 0193-1857
  • 1522-1547
DOI
10.1152/ajpgi.00490.2009
Cited by
53
References
2
Language
en

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