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Isolation from urine of pyrophosphate, a calcification inhibitor
Abstract
The minimum value of the product, Ca X P, necessary for calcium phosphate precipitation to occur, was determined in vitro. The addition of 2.2% urine to the solutions raised the mean product from 53 to 105 (mg/100 ml)2, which shows that the urine contains inhibitors to hydroxyapatite precipitation. One of these was isolated and purified. Its characteristics are those of inorganic pyrophosphate. The mean level of urinary pyrophosphate was 2.24 mg P/liter, sufficient to inhibit precipitation of hydroxyapatite. Pyrophosphate is one of the substances permitting urine to be supersaturated with calcium and phosphate; other urinary inhibitors are still to be identified. It is suggested that the phosphate compound present in the plasma which inhibits hydroxyapatite precipitation is pyrophosphate. In bone, pyrophosphatase would act by locally destroying this calcification inhibitor, thus allowing the apatite crystals to form.
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Cited by
Bisphosphonates: Mechanisms of Action
Fleisch, H. (1998) · Endocrine Reviews · doi:10.1210/edrv.19.1.0325 · Cited by 1,019
Self-citationEffect of Pyrophosphate on Hydroxyapatite and Its Implications in Calcium Homeostasis
FLEISCH, H., RUSSELL, R. G. G., & STRAUMANN, F. (1966) · Nature · doi:10.1038/212901a0 · Cited by 517
Self-citationPyrophosphate: a key inhibitor of mineralisation
Orriss, I. R., Arnett, T. R., & Russell, R. G. G. (2016) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2016.03.003 · Cited by 181
A Review of Phosphate Mineral Nucleation in Biology and Geobiology
Omelon, S., Ariganello, M., Bonucci, E., Grynpas, M., & Nanci, A. (2013) · Calcified Tissue International · doi:10.1007/s00223-013-9784-9 · Cited by 85
Pradeep, A. R., Agarwal, E., Arjun Raju, P., Rao, M. S. N., & Faizuddin, M. (2011) · Journal of Periodontology · doi:10.1902/jop.2010.100355 · Cited by 12
Metadata
- Authors
- Fleisch, H., & Bisaz, S.
- Venue
- American Journal of Physiology-Legacy Content
- Publisher
- American Physiological Society
- Published
- 1962-10-01
- Volume
- 203
- Issue
- 4
- Pages
- 671-675
- ISSN
- 0002-9513 (ISSN-L)
- OpenAlex
- W2325284371
- Cited by
- 349
- References
- 0
- FWCI
- 15.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fleisch, H., & Bisaz, S. (1962). Isolation from urine of pyrophosphate, a calcification inhibitor. American Journal of Physiology-Legacy Content. https://doi.org/10.1152/ajplegacy.1962.203.4.671
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