Effect of consecutive repeated sprint and resistance exercise bouts on acute adaptive responses in human skeletal muscle
Open accessAbstract
We examined acute molecular responses in skeletal muscle to repeated sprint and resistance exercise bouts. Six men [age, 24.7 ± 6.3 yr; body mass, 81.6 ± 7.3 kg; peak oxygen uptake, 47 ± 9.9 ml·kg−1·min−1; one repetition maximum (1-RM) leg extension 92.2 ± 12.5 kg; means ± SD] were randomly assigned to trials consisting of either resistance exercise (8 × 5 leg extension, 80% 1-RM) followed by repeated sprints (10 × 6 s, 0.75 N·m torque·kg−1) or vice-versa. Muscle biopsies from vastus lateralis were obtained at rest, 15 min after each exercise bout, and following 3-h recovery to determine early signaling and mRNA responses. There was divergent exercise order-dependent phosphorylation of p70 S6K (S6K). Specifically, initial resistance exercise increased S6K phosphorylation (∼75% P < 0.05), but there was no effect when resistance exercise was undertaken after sprints. Exercise decreased IGF-I mRNA following 3-h recovery (∼50%, P = 0.06) independent of order, while muscle RING finger mRNA was elevated with a moderate exercise order effect ( P < 0.01). When resistance exercise was followed by repeated sprints PGC-1α mRNA was increased (REX1-SPR2; P = 0.02) with a modest distinction between exercise orders. Repeated sprints may promote acute interference on resistance exercise responses by attenuating translation initiation signaling and exacerbating ubiquitin ligase expression. Indeed, repeated sprints appear to generate the overriding acute exercise-induced response when undertaking concurrent repeated sprint and resistance exercise. Accordingly, we suggest that sprint-activities are isolated from resistance training and that adequate recovery time is considered within periodized training plans that incorporate these divergent exercise modes.
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In the sprint interval study by Coffey and colleagues [50], activation of AMPK in both training groups was the same.
“the activation of AMPK in both of the training groups was the same in the sprint interval study by Coffey and colleagues”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [50]
AMPK could not explain the inhibition of mTOR activity in the sprint interval study by Coffey and colleagues [50].
“suggesting that AMPK could not explain the inhibition of mTOR activity [50].”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [50]
In Coffey et al. [50], activation of mTORC1 following eight sets of five repetitions at 80% 1RM was completely lost if subjects performed ten 6-second maximal sprint efforts on a bicycle 15 min before strength training.
“the activation of the mTORC1 following eight sets of five repetitions at 80 % of their one repetition maximum (1RM) was completely lost if the subjects had performed ten 6-second maximal sprint efforts on a bicycle 15 min before strength training”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [50]
In Coffey et al. [50], mTOR activity rapidly returned to baseline if the sprint session was performed after strength training.
“mTOR activity rapidly returned to baseline if the sprint session was performed after strength training [50].”
From Using Molecular Biology to Maximize Concurrent Training (2014) · cites this paper as [50]
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References 66 · Cited by 120
- Authors
- Coffey, V. G., Jemiolo, B., Edge, J., Garnham, A. P., Trappe, S. W., & Hawley, J. A.
- Affiliations
- RMIT University (AU)
- Human Performance Laboratory, Ball State University, Indiana;
- University of Auckland (NZ)
- Deakin University (AU)
- Ball State University (US)
- Corresponding author
- Coffey, Vernon G.
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 2009-11
- Volume
- 297
- Issue
- 5
- Pages
- R1441-R1451
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2118121521
- Cited by
- 120
- References
- 66
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 8 · 2021: 7 · 2020: 6 · 2019: 11 · 2018: 8 · 2017: 10 · 2016: 9 · 2015: 9 · 2014: 13 · 2013: 5 · 2012: 6
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- cc-by
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Victoria University Research Repository (Victoria University) (submittedVersion)
- Research Bank (Australian Catholic University) (submittedVersion)
- RMIT Research Repository (RMIT University Library) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- Australian Sports Commission
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Keywords
- repeated sprint exercise, resistance training, exercise order, skeletal muscle, IGF-I mRNA, PGC-1α, ubiquitin ligase, post-exercise recovery, training periodization, leg extension
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Adaptation, Physiological, Adolescent, Adult, Bicycling, Biopsy, Citrate (si)-Synthase, DNA-Binding Proteins, Heat-Shock Proteins, Hexokinase, Humans, Hydrogen-Ion Concentration, Insulin-Like Growth Factor I, Lactates, Male, Transcription Factors, Exercise, MyoD Protein, Muscle, Skeletal, Cross-Over Studies, Mitochondrial Proteins, Resistance Training, Young Adult
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