Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise
Open accessAbstract
Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7+cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 ± 0.04, mean ± SE) compared with the prevalue (0.07 ± 0.02, P < 0.05), whereas the number of Pax7+cells was unchanged in the leg muscles exposed to the NSAID (0.07 ± 0.01). The number of inflammatory cells (CD68+or CD16+cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.
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A number of studies have found no effect of NSAIDs on analgesia or inflammation during exercise.
“By contrast, a number of studies have found no effect of NSAIDs on analgesia or inflammation during exercise [184–188].”
From International Society of Sports Nutrition Position Stand: nutritional considerations for single-stage ultra-marathon training and racing (2019) · cites this paper as [186]
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References 48 · Cited by 185
Research works printed but not linked: 1
- [41]41. Warner DC, Schnepf G, Barrett MS, Dian D, Swigonski NL. Prevalence, attitudes, and behaviors related to the use of nonsteroidal anti-inflammatory drugs (NSAIDs) in student athletes. J Adolesc Health 30: 150–153, 2002. [DOI] [PubMed] [Google Scholar]
- Authors
- Mikkelsen, U. R., Langberg, H., Helmark, I. C., Skovgaard, D., Andersen, L. L., Kjær, M., & Mackey, A. L.
- Affiliations
- University of Copenhagen (DK)
- National Research Centre for the Working Environment (DK)
- Corresponding author
- Mikkelsen, U. R.
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2009-11
- Volume
- 107
- Issue
- 5
- Pages
- 1600-1611
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- PMC
- PMC3774508
- OpenAlex
- W2068146091
- Cited by
- 185
- References
- 48
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 6 · 2023: 7 · 2022: 5 · 2021: 8 · 2020: 10 · 2019: 9 · 2018: 18 · 2017: 20 · 2016: 13 · 2015: 15 · 2014: 14 · 2013: 16 · 2012: 16
- FWCI
- 10.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Applied Physiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- nonsteroidal anti-inflammatory drugs, satellite cells, skeletal muscle, eccentric exercise, Pax7, CD56, muscle regeneration, microdialysis, vastus lateralis, inflammatory cells, exercise-induced muscle damage
- MeSH terms
- Adult, Anti-Inflammatory Agents, Non-Steroidal, Physical Exertion, Humans, Indomethacin, Infusions, Parenteral, Injections, Intramuscular, Male, Physical Endurance, Cytokines, Muscle, Skeletal, Satellite Cells, Skeletal Muscle, Cell Proliferation, Young Adult
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