Increased susceptibility to Pseudomonas aeruginosa infection under hindlimb-unloading conditions
Abstract
It has been reported that spaceflight conditions alter the immune system and resistance to infection [Belay T, Aviles H, Vance M, Fountain K, and Sonnenfeld G. J Allergy Clin Immunol 170: 262-268, 2002; Hankins WR and Ziegelschmid JF. In: Biomedical Results of Apollo. Washington, DC: NASA, 1975, p. 43-81. (NASA Spec. Rep. SP-368)]. Ground-based models, including the hindlimb-unloading model, have become important tools for increasing understanding of how spaceflight conditions can influence physiology. The objective of the present study was to determine the effect of hindlimb unloading on the susceptibility of mice to Pseudomonas aeruginosa infection. Hindlimb-unloaded and control mice were subcutaneously infected with 1 LD50 of P. aeruginosa. Survival, bacterial organ load, and antibody and corticosterone levels were compared among the groups. Hindlimb unloading had detrimental effects for infected mice. Animals in the hindlimb-unloaded group, compared with controls, 1). showed significantly increased mortality and reduced time to death, 2). had increased levels of corticosterone, and 3). were much less able to clear bacteria from the organs. These results suggest that hindlimb unloading may induce the production of corticosterone, which may play a critical role in the modulation of the immune system leading to increased susceptibility to P. aeruginosa infection.
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Animal models of space flight have demonstrated a reduced ability to clear infections by Klebsiella pneumoniae and Pseudomonas aeruginosa.
“Animal models of space flight have demonstrated a reduced ability to clear infections by Klebsiella pneumoniae [24] and Pseudomonas aeruginosa [25].”
From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [25]
Studies have indicated a reduction in the ability of hindlimb unloaded mice to control bacterial infections.
“Studies have indicated a reduction in the ability of hindlimb unloaded mice to control bacterial infections.”
From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [25]
The studies of systemic Klebsiella pneumoniae and Pseudomonas aeruginosa gave mice an LD50 of bacteria, which allowed small changes in immune function to result in significant increases in mortality above the expected 50%.
“The studies of systemic Klebsiella pneumoniae and Pseudomonas aeruginosa gave mice an LD50 of bacteria [24], [25], which allowed small changes in immune function to result in significant increases in mortality above the expected 50%.”
From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [25]
Suspended mice given subcutaneous Pseudomonas aeruginosa had increased mortality and organ bacterial load and an increase in corticosterone.
“Suspended mice given subcutaneous Pseudomonas aeruginosa had increased mortality and organ bacterial load and an increase in corticosterone [25].”
From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [25]
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References 37 · Cited by 53
- Authors
- Aviles, H., Belay, T., Fountain, K., Vance, M., & Sonnenfeld, G.
- Affiliations
- Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30310-1495
- Morehouse School of Medicine (US)
- ORCID
- Belay, Tesfaye
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2003-07
- Volume
- 95
- Issue
- 1
- Pages
- 73-80
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2137856862
- Cited by
- 53
- References
- 37
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 5 · 2023: 3 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Center for Research Resources (5G12 RR/AI 03034)
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Surgery › Cardiovascular Syncope and Autonomic Disorders
- Keywords
- Pseudomonas aeruginosa, hindlimb unloading, spaceflight, infection susceptibility, corticosterone, immunomodulation, bacterial clearance, survival, mice, microgravity
- MeSH terms
- Animals, Antibodies, Bacterial, Corticosterone, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulin G, Immunoglobulin M, Kinetics, Pseudomonas Infections, Time Factors, Survival Analysis, Hindlimb Suspension, Mice
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