Temporal Regulation of the Expression Locus of Homeostatic Plasticity
Abstract
Homeostatic plasticity of excitatory synapses plays an important role in stabilizing neuronal activity, but the mechanism of this form of plasticity is incompletely understood. In particular, whether the locus of expression is presynaptic or postsynaptic has been controversial. Here we show that the expression locus depends on the time neurons have spent in vitro. In visual cortical cultures or =18 DIV, the same TTX treatment induced a large increase in mEPSC frequency, whereas the amplitude effect was reduced. The increased mEPSC frequency was associated with an increased density of excitatory synapses and increased presynaptic vesicle release in response to electrical stimulation. This indicates a shift from a predominantly postsynaptic response to TTX in or =18 DIV cultures. This shift was not specific for cortical cultures because a similar shift was observed in cultured hippocampal neurons. Culturing neurons from older animals showed that the timing of the switch depends on the time the neurons have spent in vitro, rather than their postnatal age. This temporal switch in expression locus can largely reconcile the contradictory literature on the expression locus of homeostatic excitatory synaptic plasticity in central neurons. Furthermore, our results raise the intriguing possibility that the expression mechanism of homeostatic plasticity can be tailored to the needs of the network during different stages of development or in response to different challenges to network fu…
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The same manipulation (activity blockade using TTX or AMPAR antagonists) that only affects mini amplitude in young cultures can also induce changes in mini frequency and presynaptic release probability in older neuronal cultures, through a process involving postsynaptic hyperpolarization and a drop in calcium influx.
“Interestingly, the same manipulation (activity blockade using TTX or AMPAR antagonists) that only affects mini amplitude in young cultures (Turrigiano et al. 1998;Wierenga et al. 2005) can also induce changes in mini frequency and in presynaptic release probability in older neuronal cultures (Wierenga et al. 2006), through a process that involves postsynaptic hyperpolarization and a drop in”
From Homeostatic Synaptic Plasticity: Local and Global Mechanisms for Stabilizing Neuronal Function (2012) · cites this paper as Wierenga et al. 2006
Given that postsynaptic and presynaptic responses to activity deprivation can occur independently, they likely represent distinct forms of plasticity induced by distinct signaling pathways.
“Given that the postsynaptic and presynaptic responses to activity deprivation can occur independently (Wierenga et al. 2005(Wierenga et al. , 2006)), it is likely that they represent distinct forms of plasticity induced by distinct signaling pathways.”
From Homeostatic Synaptic Plasticity: Local and Global Mechanisms for Stabilizing Neuronal Function (2012) · cites this paper as Wierenga et al. 2006
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References 34 · Cited by 175
- Authors
- Wierenga, C. J., Walsh, M. F., & Turrigiano, G. G.
- Affiliations
- Brandeis University (US)
- Venue
- Journal of Neurophysiology
- Publisher
- American Physiological Society
- Published
- 2006-10
- Volume
- 96
- Issue
- 4
- Pages
- 2127-2133
- ISSN
- 0022-3077 (ISSN-L)
- 1522-1598
- OpenAlex
- W1971402245
- Cited by
- 175
- References
- 34
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 8 · 2021: 7 · 2020: 6 · 2019: 9 · 2018: 5 · 2017: 7 · 2016: 6 · 2015: 10 · 2014: 10 · 2013: 14 · 2012: 14
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurophysiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS 36853)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- homeostatic plasticity, excitatory synapses, presynaptic plasticity, postsynaptic plasticity, miniature excitatory postsynaptic currents, tetrodotoxin, hippocampal neurons, synaptic density, neuronal development, network stability
- MeSH terms
- Anesthetics, Local, Animals, Cells, Cultured, Electric Stimulation, Homeostasis, Synaptic Transmission, Neuronal Plasticity, Neurons, Rats, Inbred Strains, Tetrodotoxin, Visual Cortex, Cellular Senescence, Gene Expression Regulation, Developmental, Excitatory Postsynaptic Potentials, Rats
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