The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Imaging Neuronal Activity During Zebrafish Behavior With a Genetically Encoded Calcium Indicator
Abstract
Genetically encoded calcium indicators, such as cameleon, have offered the promise of noninvasively monitoring activity of neurons, but no one has demonstrated whether these indicators can report calcium transients in neurons of behaving vertebrates. We show that cameleon can be expressed at high levels in sensory and spinal cord neurons in zebrafish by using neural-specific promoters in both transient expression experiments and in a stable transgenic line. Using standard confocal microscopy, calcium transients in identified motoneurons and spinal interneurons could be detected during escape behaviors produced by a touch on the head of the fish. Small movements of the restrained fish during the behavior did not represent a major problem for analyzing the calcium responses because of the ratiometric nature of cameleon. We conclude that cameleon can be used to noninvasively study the activity of neurons in an intact, behaving vertebrate. The ability to introduce an indicator genetically allows for studies of the functional roles of local interneurons that cannot easily be monitored with other approaches. Transgenic lines such as the one we generated can also be crossed into mutant lines of fish to study both structural and functional consequences of the mutations.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Miyawaki, A., Llopis, J., Heim, R., McCaffery, J. M., Adams, J. A., Ikura, M., & Tsien, R. Y. (1997) · Nature · doi:10.1038/42264 · Cited by 3,248
A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein
Nakai, J., Ohkura, M., & Imoto, K. (2001) · Nature Biotechnology · doi:10.1038/84397 · Cited by 1,616
Circularly permuted green fluorescent proteins engineered to sense Ca 2+
Nagai, T., Sawano, A., Park, E. S., & Miyawaki, A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.051636098 · Cited by 966
Dynamic and quantitative Ca 2+ measurements using improved cameleons
Miyawaki, A., Griesbeck, O., Heim, R., & Tsien, R. Y. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.5.2135 · Cited by 823
Polymodal Motion Processing in Posterior Parietal and Premotor Cortex
Bremmer, F., Schlack, A., Shah, N. J., Zafiris, O., Kubischik, M., Hoffmann, K.-P., Zilles, K., & Fink, G. R. (2001) · Neuron · doi:10.1016/s0896-6273(01)00198-2 · Cited by 803
Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain
Wang, J. W., Wong, A. M., Flores, J., Vosshall, L. B., & Axel, R. (2003) · Cell · doi:10.1016/s0092-8674(03)00004-7 · Cited by 801
Genes controlling and mediating locomotion behavior of the zebrafish embryo and larva
Granato, M., Eeden, F. J. M. V., Schach, U., Trowe, T., Brand, M., Furutani-Seiki, M., Haffter, P., Hammerschmidt, M., Heisenberg, C.-P., Jiang, Y.-J., et al. (1996) · Development · doi:10.1242/dev.123.1.399 · Cited by 644
Higashijima, S.-I., Hotta, Y., & Okamoto, H. (2000) · The Journal of Neuroscience · doi:10.1523/jneurosci.20-01-00206.2000 · Cited by 559
Self-citationLocomotor repertoire of the larval zebrafish: swimming, turning and prey capture
Budick, S. A., & O’Malley, D. M. (2000) · Journal of Experimental Biology · doi:10.1242/jeb.203.17.2565 · Cited by 499
Park, H.-C., Kim, C.-H., Bae, Y.-K., Yeo, S.-Y., Kim, S.-H., Hong, S.-K., Shin, J., Yoo, K.-W., Hibi, M., Hirano, T., et al. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9898 · Cited by 458
Cited by
Guidelines on nicotine dose selection for in vivo research
Matta, S. G., Balfour, D. J., Benowitz, N. L., Boyd, R. T., Buccafusco, J. J., Caggiula, A. R., Craig, C. R., Collins, A. C., Damaj, M. I., Donny, E. C., et al. (2007) · Psychopharmacology · doi:10.1007/s00213-006-0441-0 · Cited by 824
Brain-wide neuronal dynamics during motor adaptation in zebrafish
Ahrens, M. B., Li, J. M., Orger, M. B., Robson, D. N., Schier, A. F., Engert, F., & Portugues, R. (2012) · Nature · doi:10.1038/nature11057 · Cited by 729
Hypocretin/orexin overexpression induces an insomnia-like phenotype in zebrafish
Prober, D. A., Rihel, J., Onah, A. A., Sung, R.-J., & Schier, A. F. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.4332-06.2006 · Cited by 508
Ca2+ Indicators Based on Computationally Redesigned Calmodulin-Peptide Pairs
Palmer, A. E., Giacomello, M., Kortemme, T., Hires, S. A., Lev-Ram, V., Baker, D., & Tsien, R. Y. (2006) · Chemistry & Biology · doi:10.1016/j.chembiol.2006.03.007 · Cited by 508
Bozza, T., McGann, J. P., Mombaerts, P., & Wachowiak, M. (2004) · Neuron · doi:10.1016/s0896-6273(04)00144-8 · Cited by 356
Metadata
- Authors
- Higashijima, S.-I., Masino, M. A., Mandel, G., & Fetcho, J. R.
- Venue
- Journal of Neurophysiology
- Publisher
- American Physiological Society
- Published
- 2003-12
- Volume
- 90
- Issue
- 6
- Pages
- 3986-3997
- ISSN
- 0022-3077 (ISSN-L)
- 1522-1598
- OpenAlex
- W2126672460
- Cited by
- 264
- References
- 33
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Imaging Neuronal Activity During Zebrafish Behavior With a Genetically Encoded Calcium Indicator. https://app.cochat.ai/papers/10.1152/jn.00576.2003
- The paper
- Higashijima, S.-I., Masino, M. A., Mandel, G., & Fetcho, J. R. (2003). Imaging Neuronal Activity During Zebrafish Behavior With a Genetically Encoded Calcium Indicator. Journal of Neurophysiology. https://doi.org/10.1152/jn.00576.2003
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
