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Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in Human and Other Primate Cell Lines

Uphoff, Cord C., Denkmann, Sabine A., Steube, Klaus G., Drexler, Hans G.·2010·Journal of Biomedicine and Biotechnology
DOI 10.1155/2010/904767PMC PMC2861168
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Abstract

The high prevalence of contaminated cell cultures suggests that viral contaminations might be distributed among cultures. We investigated more than 460 primate cell lines for Epstein-Barr (EBV), hepatitis B (HBV), hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), human T-cell leukemia/lymphoma virus I and II (HTLV-I/-II), and squirrel monkey retrovirus (SMRV) infections for risk assessment. None of the cell lines were infected with HCV, HIV-1, or HTLV-I/-II. However, one cell line displayed reverse transcriptase activity. Thirty-nine cell lines harbored EBV DNA sequences. Studies on the lytic phase of EBV revealed that five cell lines produce EBV particles and six further cell lines produced EBV upon stimulation. One cell line contained an integrated HBV genome fragment but showed no virus production. Six cell lines were SMRV-infected. Newly established cell lines should be tested for EBV infections to detect B-lymphoblastoid cell lines (B-LCL). B-LCLs established with EBV from cell line B95-8 should be tested for SMRV infections.

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  • Contamination with microorganisms and viruses can result in the catastrophic loss of cultures, and contaminations may potentially spread and infect other cultures, lab personnel and operators in a laboratory.

    “Contamination with microorganisms and viruses can result in the catastrophic loss of cultures, and contaminations may potentially spread and infect other cultures, lab personnel and operators in a laboratory (Uphoff et al., 2010).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Uphoff et al., 2010

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Authors
Uphoff, C. C., Denkmann, S. A., Steube, K. G., & Drexler, H. G.
Affiliations
Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures DSMZ, Inhoffenstraße 7b; 38124 Braunschweig, Germany
Venue
Journal of Biomedicine and Biotechnology
Publisher
Hindawi Limited
Published
2010
Volume
2010
Pages
1-23
ISSN
  • 1110-7243
  • 1110-7251
DOI
10.1155/2010/904767
PMC
PMC2861168
Cited by
41
References
0
Language
en
Licence
creativecommons.org/licenses/by/3.0/ (unspecified)

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