Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs
Open accessAbstract
Centella asiatica herbs have been prescribed as a traditional medicine for wound healing in China and Southeast Asia for a long time. They contain many kinds of triterpenoid compounds, mainly including glycosides (asiaticoside and madecassoside) and corresponding aglycones (asiatic acid and madecassic acid). To identify which is the major active constituent, a comprehensive and comparative study of these compounds was performed.In vitro, primary human skin fibroblasts, originating from healthy human foreskin samples, were treated with various concentrations of asiaticoside, madecassoside, asiatic acid, and madecassic acid, respectively. Cell proliferation, collagen synthesis, MMP-1/TIMP-1 balance, and TGF-β/Smad signaling pathway were investigated.In vivo, mice were orally administered with the four compounds mentioned above for two weeks after burn injury. The speed and quality of wound healing, as well as TGF-β1 levels in skin tissues, were examined. Interestingly, in contrast to prevalent postulations, asiaticoside and madecassoside themselves, rather than their corresponding metabolites asiatic acid and madecassic acid, are recognized as the main active constituents of C. asiatica herbs responsible for burn wound healing. Furthermore, madecassoside is more effective than asiaticoside (P=0.0446for procollagen type III synthesis in vitro,P=0.0057for wound healing speed, andP=0.0491for wound healing pattern in vivo, correspondingly).
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Asiaticoside and madecassoside have collagen-stimulating, hydrating, scar and burn-healing properties.
“Furthermore, these compounds have collagen‐stimulating, hydrating, scar and burn‐healing properties, 35 , 36”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [35]
Results §§3.2 and 3.5 (page not provided) report increased collagen synthesis in human skin fibroblasts and faster burn-wound healing in mice with asiaticoside and madecassoside. The paper does not establish hydrating properties or specifically test scar healing; its mention of scars in the Introduction concerns abnormal wound repair generally. (LLM, conf=0.88)
Experimental — accuracy not yet measuredAsiaticoside and madecassoside induce collagen production and thereby prevent skin-ageing caused by low Type 1 collagen secretion in dermis.
“Instead, asiaticoside and madecassoside prove cosmetically useful by inducing the production of collagen and thus preventing skin‐ageing, which is caused as a result of low Type 1 collagen secretion in the dermis.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [35]
Section 3.2 reports that asiaticoside and madecassoside increased type I and III collagen markers in human skin fibroblasts. The paper studies burn-wound healing, not cosmetic use or prevention of skin ageing; it also does not establish that low type I collagen secretion in the dermis causes ageing. Page numbers are not available in the supplied passages. (LLM, conf=0.90)
Experimental — accuracy not yet measuredBoth asiaticoside and madecassoside stimulate collagen production in context of burn wound healing.
“Both asiaticoside and madecassoside have been observed to stimulate collagen production in the context of burn wound healing.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [35]
Orally administered madecassoside shows significantly higher efficiency in synthesis of procollagen, wound-healing speed and wound-healing pattern in vitro in primary skin fibroblasts and in vivo in mice with burn injuries.
“orally administrated madecassoside shows significantly higher efficiency in the synthesis of procollagen, wound‐healing speed and wound‐healing pattern, as observed in vitro in primary skin fibroblasts and in vivo in mice that have sustained burn injuries.”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [35]
Asiaticoside and madecassoside are main active constituents involved in healing burns.
“Asiaticoside and madecassoside are recognized as the main active constituents involved in healing burns”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [35]
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References 30 · Cited by 105
Research works printed but not linked: 23
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- Authors
- Wu, F., Bian, D., Xia, Y., Gong, Z., Tan, Q., Chen, J., & Dai, Y.
- Affiliations
- China Pharmaceutical University (CN)
- Nanjing Normal University (CN)
- Nanjing Drum Tower Hospital (CN)
- Corresponding author
- Dai, Yue
- Venue
- Evidence-Based Complementary and Alternative Medicine
- Publisher
- Wiley
- Published
- 2012
- Volume
- 2012
- Pages
- 1-13
- ISSN
- 1741-427X (ISSN-L)
- 1741-4288
- PMC
- PMC3546525
- OpenAlex
- W2074498845
- Cited by
- 105
- References
- 30
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 9 · 2023: 9 · 2022: 7 · 2021: 15 · 2020: 10 · 2019: 5 · 2018: 11 · 2017: 5 · 2016: 1 · 2015: 6 · 2014: 8 · 2013: 6
- FWCI
- 15.58
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Evidence-based Complementary and Alternative Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (30973915)
- Jiangsu Higher Education Institutions (30973915)
- Innovative Research Plan for Undergraduate Students of China Pharmaceutical University (30973915)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- Centella asiatica, burn wound healing, asiaticoside, madecassoside, asiatic acid, madecassic acid, triterpenoid saponins, dermal fibroblasts, collagen synthesis, TGF-β/Smad signaling, active components, traditional medicine
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