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miRNA Transcriptome of Hypertrophic Skeletal Muscle with Overexpressed Myostatin Propeptide

Javed, Ruheena, Jing, Lu, Yang, Jinzeng, Li, Xinyun, Cao, Jianhua, Zhao, Shuhong·2014·BioMed Research International
DOI 10.1155/2014/328935PMC PMC4131533
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Abstract

MicroRNAs (miRNAs) play an imperative role in cell proliferation, differentiation, and cell metabolism through regulation of gene expression. Skeletal muscle hypertrophy that results from myostatin depression by its propeptide provides an interesting model to understand how miRNA transcriptome is involved in myostatin-based fiber hypertrophy. This study employed Solexa deep sequencing followed by Q-PCR methods to analyze miRNA transcriptome of skeletal muscle of myostatin propeptide transgenic mice in comparison with their littermate controls. A total of 461 mature known and 69 novel miRNAs were reported from this study. Fifty-seven miRNAs were expressed differentially between transgenic and littermate controls, of which most abundant miRNAs, miR-133a and 378a, were significantly differentially expressed. Expression profiling was validated on 8 known and 2 novel miRNAs. The miRNA targets prediction and pathway analysis showed that FST, SMAD3, TGFBR1, and AcvR1a genes play a vital role in skeletal muscle hypertrophy in the myostatin propeptide transgenic mice. It is predicted that miR-101 targeted to TGFBR1 and SMAD3, miR-425 to TGFBR2 and FST, and miR-199a to AcvR2a and TGF-β genes. In conclusion, the study offers initial miRNA profiling and methodology of miRNA targets prediction for myostatin-based hypertrophy. These differentially expressed miRNAs are proposed as candidate miRNAs for skeletal muscle hypertrophy.

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  • 87 out of 473 novel miRNA were previously reported within other studies.

    “We also found that 87 out of 473 novel miRNA were previously reported within other studies (48–52)”

    From A mouse tissue atlas of small noncoding RNA (2020) · cites this paper as [50]

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Cited by

Sorted by citations

Authors
Javed, R., Jing, L., Yang, J., Li, X., Cao, J., & Zhao, S.
Affiliations
  • Department of Animal Genetics & Breeding, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China
  • Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
ORCID
Venue
BioMed Research International
Publisher
Wiley
Published
2014
Volume
2014
Pages
1-19
ISSN
  • 2314-6133
  • 2314-6141
DOI
10.1155/2014/328935
PMC
PMC4131533
Cited by
11
References
2
Language
en
Licence
creativecommons.org/licenses/by/3.0/ (unspecified)
Funders
  • Chinese National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2013ZX-08006005, 31025026, 13P-60310)
  • Fundamental Research Funds for the Central Universities of China (2013ZX-08006005, 31025026, 13P-60310)
  • National Natural Science Foundation of China (2013ZX-08006005, 31025026, 13P-60310)
  • Hawaii Community Foundation (2013ZX-08006005, 31025026, 13P-60310)

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