Direct Cell-Cell Contact between Mesenchymal Stem Cells and Endothelial Progenitor Cells Induces a Pericyte-Like Phenotype In Vitro
Open accessAbstract
Tissue engineering techniques for the regeneration of large bone defects require sufficient vascularisation of the applied constructs to ensure a sufficient supply of oxygen and nutrients. In our previous work, prevascularised 3D scaffolds have been successfully established by coculture of bone marrow derived stem cells (MSCs) and endothelial progenitor cells (EPCs). We identified stabilising pericytes (PCs) as part of newly formed capillary-like structures. In the present study, we report preliminary data on the interactions between MSCs and EPCs, leading to the differentiation of pericyte-like cells. MSCs and EPCs were seeded in transwell cultures, direct cocultures, and single cultures. Cells were cultured for 10 days in IMDM 10% FCS or IMDM 5% FCS 5% platelet lysate medium. Gene expression of PC markers, CD146, NG2, αSMA, and PDGFR-β, was analysed using RT-PCR at days 0, 3, 7, and 10. The upregulation of CD146, NG2, and αSMA in MSCs in direct coculture with EPCs advocates the MSCs' differentiation towards a pericyte-like phenotype in vitro. These results suggest that pericyte-like cells derive from MSCs and that cell-cell contact with EPCs is an important factor for this differentiation process. These findings emphasise the concept of coculture strategies to promote angiogenesis for cell-based tissue engineered bone grafts.
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Co-culturing MSCs with EPCs induced differentiation into pericyte-like cells that stabilize newly formed vasculature.
“Furthermore, by co-culturing with EPCs, MSCs were induced to differentiate into pericyte-like cells, which act to stabilize the newly formed vasculature [69, 375].”
From Mechanisms, efficacy, and future perspectives of cellular-based therapies for liver fibrosis/cirrhosis: focusing on mesenchymal stromal cells (2025) · cites this paper as [375]
The Conclusion reports that coculture with EPCs induced MSCs toward a pericyte-like phenotype in vitro, based on increased pericyte-marker expression. The Introduction describes pericytes as contributing to microvessel stability, but this study does not show that the induced cells stabilize newly formed vasculature. (LLM, conf=0.85)
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References 34 · Cited by 94
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- Authors
- Loibl, M., Binder, A., Herrmann, M., Duttenhoefer, F., Richards, R. G., Nerlich, M., Alini, M., & Verrier, S.
- Affiliations
- AO Foundation (CH)
- University of Freiburg (DE)
- University Hospital Regensburg (DE)
- Corresponding author
- Verrier, Sophie
- ORCID
- Venue
- BioMed Research International
- Publisher
- Wiley
- Published
- 2014
- Volume
- 2014
- Pages
- 1-10
- ISSN
- 1110-7243 (ISSN-L)
- 2314-6133
- 2314-6141
- PMC
- PMC3915932
- OpenAlex
- W1999010820
- Cited by
- 94
- References
- 34
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1 · 2023: 3 · 2022: 11 · 2021: 14 · 2020: 7 · 2019: 12 · 2018: 2 · 2017: 10 · 2016: 10 · 2015: 10 · 2014: 7
- FWCI
- 6.19
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- BioMed Research International (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- University of Regensburg Publication Server (University of Regensburg) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- University of Regensburg Publication Server (University of Regensburg)
- Funders
- Deutsche Forschungsgemeinschaft
- AO Foundation
- Innovationsstiftung Graubünden
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- mesenchymal stem cells, endothelial progenitor cells, pericyte-like cells, cell-cell contact, co-culture, pericyte differentiation, CD146, NG2, alpha-smooth muscle actin, PDGFRβ, angiogenesis, bone tissue engineering, bone regeneration, platelet lysate
- MeSH terms
- Chondroitin Sulfate Proteoglycan 4, Actins, Adult, Aged, Antigens, Cell Communication, Cells, Cultured, Culture Media, Female, Gene Expression Regulation, Humans, Male, Middle Aged, Phenotype, Proteoglycans, Biomarkers, Coculture Techniques, Pericytes, CD146 Antigen, Young Adult, Mesenchymal Stem Cells, Endothelial Progenitor Cells
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