Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota
Open accessAbstract
The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1 β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion,L. kefiri is a good candidate to be used in gut inflammatory disorders.
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Supplementation of L. kefiri CIDCA 8348 results in increased gene expression of IL-10 in the ileum and MLNs in mice.
“Notably, supplementation of L. kefiri CIDCA 8348 results in increased gene expression of IL-10 in the ileum and MLNs in mice [15].”
From Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse (2020) · cites this paper as [15]
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References 75 · Cited by 145
- Authors
- Carasi, P., Racedo, S. M., Jacquot, C., Romanin, D. E., Serradell, M. A., & Urdaci, M. C.
- Affiliations
- Université de Bordeaux (FR)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Cátedra de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115 s/n, 1900 La Plata, Argentina
- Corresponding author
- Urdaci, M. C.
- Venue
- Journal of Immunology Research
- Publisher
- Wiley
- Published
- 2015
- Volume
- 2015
- Pages
- 1-12
- ISSN
- 2314-7156 (ISSN-L)
- 2314-8861
- PMC
- PMC4355334
- OpenAlex
- W2068418325
- Cited by
- 145
- References
- 75
- Citations by year
- 2026: 6 · 2025: 18 · 2024: 8 · 2023: 15 · 2022: 20 · 2021: 21 · 2020: 13 · 2019: 11 · 2018: 8 · 2017: 10 · 2016: 12 · 2015: 3
- FWCI
- 14.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Immunology Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
- Greater South Information System
- Funders
- Agencia Nacional de Promoción Científica y Tecnológica (PICT 00479/06, 11X/548)
- Universidad Nacional de La Plata (PICT 00479/06, 11X/548)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus kefiri, kefir, probiotics, mucosal immunity, gut microbiota, IgA, IL-10, inflammatory cytokines, Peyer's patches, mesenteric lymph nodes, intestinal inflammation, lipopolysaccharide-induced inflammation, mucin gene expression, fecal microbiota, anti-inflammatory activity
- MeSH terms
- Gastrointestinal Microbiome, Animals, Cell Line, Feces, Gene Expression Regulation, Humans, Immunoglobulin A, Intestinal Mucosa, Lactobacillus, Leukocytes, Mononuclear, Male, Gene Expression, Lymphocyte Subsets, Cytokines, Genes, Reporter, Immunity, Mucosal, Cultured Milk Products, Mice
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