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Cited by 16Curcumin's Biomedical ApplicationsDupuytren's Contracture and TreatmentsNatural Compounds in Disease Treatment

Curcumin Inhibits Joint Contracture through PTEN Demethylation and Targeting PI3K/Akt/mTOR Pathway in Myofibroblasts from Human Joint Capsule

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Zhuang, Ze, Yu, Dongjie, Chen, Zheng, Liu, Dezhao, Yuan, Guohui, Yirong, Ni, Sun, Linlin, Liu, Yuangao +2 more·2019·Evidence-Based Complementary and Alternative Medicine
DOI 10.1155/2019/4301238PMC PMC6712967
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Abstract

Joint contracture is increasingly regarded as a clinical problem that leads to irreversible dysfunction of the joint. It is a pathophysiological process following joint injury, which is marked by the activation of myofibroblasts. There is currently no effective treatment for the prevention of joint contracture. Curcumin is a polyphenol pigment extracted from turmeric, which possesses anti-inflammatory, antioxidative, and antitumor properties. In the present study, we demonstrated that curcumin exerts a protective effect against joint contracture via the inhibition of myofibroblast proliferation and migration in a time- and concentration-dependent manner. Moreover, we indicated that phosphatase and tension homolog (PTEN) was downregulated in myofibroblasts in vitro and in the contracture capsule tissues of patients in vivo. Additionally, western blot analysis revealed a negative correlation between the expression levels of PTEN and the fibrosis marker protein alpha smooth muscle cell actin. Methylation-specific PCR results suggested that curcumin was able to demethylate PTEN in a similar manner to the demethylation agent 5-azacytidine, increasing PTEN expression and further inhibiting phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling. In conclusion, our data illustrate part of the mechanism of curcumin inhibition in joint contracture. These results support the hypothesis that curcumin may potentially be used as a novel candidate for the treatment of joint contracture.

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Authors
Zhuang, Z., Yu, D., Chen, Z., Liu, D., Yuan, G., Yirong, N., Sun, L., Liu, Y., He, R., & Wang, K.
Affiliations
  • Third Affiliated Hospital of Sun Yat-sen University (CN)
  • First Affiliated Hospital of Jinan University (CN)
  • MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, Guangdong, China
Corresponding author
  • He, Ronghan
  • Wang, Kun
ORCID
Venue
Evidence-Based Complementary and Alternative Medicine
Publisher
Wiley
Published
2019-08-14
Volume
2019
Pages
1-12
ISSN
  • 1741-427X (ISSN-L)
  • 1741-4288
DOI
10.1155/2019/4301238
PMC
PMC6712967
OpenAlex
W2968304812
Cited by
16
References
0
Citations by year
2025: 3 · 2024: 1 · 2023: 1 · 2022: 7 · 2021: 3 · 2020: 1
FWCI
0.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Evidence-based Complementary and Alternative Medicine (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Guangdong Medical Science and Technology Research (A2016066, 20181061, 81772368, 81802184, 2017A030310226, A2017003)
  • Traditional Chinese Medicine Bureau of Guangdong Province (A2016066, 20181061, 81772368, 81802184, 2017A030310226, A2017003)
  • National Natural Science Foundation of China (A2016066, 20181061, 81772368, 81802184, 2017A030310226, A2017003)
  • Natural Science Foundation of Guangdong Province (A2016066, 20181061, 81772368, 81802184, 2017A030310226, A2017003)
  • Guangdong Medical Research Foundation (A2016066, 20181061, 81772368, 81802184, 2017A030310226, A2017003)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Curcumin's Biomedical Applications
  • Health Sciences › Medicine › Rheumatology › Dupuytren's Contracture and Treatments
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
Keywords
curcumin, joint contractures, myofibroblasts, joint capsule, PI3K/AKT/mTOR pathway, myofibroblast proliferation, myofibroblast migration, alpha-smooth muscle actin, joint fibrosis, azacitidine

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